FastQCFastQC Report
Thu 19 Sep 2019
HT5VLAFXY_n01_13YCol_14YCol_15YCol_16YCol_lib_k-b.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHT5VLAFXY_n01_13YCol_14YCol_15YCol_16YCol_lib_k-b.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences482558
Sequences flagged as poor quality0
Sequence length76
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ACTCTATAGAGTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGAGA195934.060237318622839TruSeq Adapter, Index 7 (97% over 37bp)
ACTCTATTGAGTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGAGA113872.3597163449782204TruSeq Adapter, Index 7 (97% over 37bp)
ACCGCATCGGAAGAGCACACGTCTGAACTCCAGTCACGAGATTCCATCTC79761.6528583092602338TruSeq Adapter, Index 9 (97% over 35bp)
ACTCTATCGGAAGAGCACACGTCTGAACTCCAGTCACGAGATTCCATCTC18890.3914555348787089TruSeq Adapter, Index 9 (97% over 35bp)
ACTCTATTAGAGTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGAG16330.3384049171291327TruSeq Adapter, Index 7 (97% over 36bp)
GTGAGGTCGGAAGAGCACACGTCTGAACTCCAGTCACGAGATTCCATCTC16050.3326025058127728TruSeq Adapter, Index 9 (97% over 35bp)
ACCGCATGGAAGAGCACACGTCTGAACTCCAGTCACGAGATTCCATCTCG12130.2513687473837342TruSeq Adapter, Index 9 (96% over 32bp)
ACTCTATAGAGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGAGAT12080.25033260250581274TruSeq Adapter, Index 7 (97% over 38bp)
ACTCTATAGATAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGAGAT6670.13822172671471616TruSeq Adapter, Index 7 (97% over 38bp)
ACCGCATCGGTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGAGAT5420.11231810476668089TruSeq Adapter, Index 7 (97% over 38bp)
ACCGCATAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGAGATTCCA4980.10320002984097248TruSeq Adapter, Index 7 (97% over 38bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGATCGT98300.069.601171
GTGAGGT100850.069.507821
CCGCATT24500.069.28532
ACCGCAT126050.069.2230451
CCGCATA43050.069.1865842
ACTCTAT141100.069.0361
CCGCATG23100.068.6359562
CGCATAC6150.068.292283
GATCGTA37650.068.233322
CTCTATA63200.067.673652
GATCGTC22050.067.618652
GCATCGG12450.067.469484
TGAGGTC23100.067.423842
GATCGTT21200.067.3580862
CGCATCG15500.067.2899253
TGAGGTA43650.067.273372
TGAGGTG20200.067.2273252
CCGCATC39600.066.817792
CGCATAG10000.066.49963
CGCATAT15050.066.278683
Produced by FastQC (version 0.11.5)