FastQCFastQC Report
Thu 19 Sep 2019
HT5VLAFXY_n01_13YCol_14YC24_lib_k-b.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHT5VLAFXY_n01_13YCol_14YC24_lib_k-b.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9035
Sequences flagged as poor quality0
Sequence length76
%GC40

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGAGATTCCATCTCGTA1481.6380741560597676TruSeq Adapter, Index 7 (97% over 38bp)
CGCGGAAGAGCACACGTCTGAACTCCAGTCACGAGATTCCATCTCGTATG630.6972883231876037TruSeq Adapter, Index 7 (97% over 35bp)
ACTCTATAGAGTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGAGA490.5423353624792473TruSeq Adapter, Index 7 (97% over 37bp)
CGTCGGAAGAGCACACGTCTGAACTCCAGTCACGAGATTCCATCTCGTAT390.4316546762589928TruSeq Adapter, Index 7 (97% over 36bp)
CGGGAAGAGCACACGTCTGAACTCCAGTCACGAGATTCCATCTCGTATGC350.387382401770891TruSeq Adapter, Index 7 (97% over 34bp)
CGGAAGAGCACACGTCTGAACTCCAGTCACGAGATTCCATCTCGTATGCC270.29883785279468733TruSeq Adapter, Index 7 (97% over 35bp)
ACTCTATTGAGTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGAGA240.26563364692861097TruSeq Adapter, Index 7 (97% over 37bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGAGATTCCATCTCGTAT220.24349750968456005TruSeq Adapter, Index 7 (97% over 38bp)
CGTGGAAGAGCACACGTCTGAACTCCAGTCACGAGATTCCATCTCGTATG210.2324294410625346TruSeq Adapter, Index 9 (96% over 32bp)
GGAAGGGGTTCTTCAATTCTCATCCTTGAAAGATGGTCAGCTGCACCATT180.19922523519645824No Hit
ACTTCATAAAATAAATTTCAGGGTAAAGACGGTAGTGATGGGGAAGAGTT150.16602102933038185No Hit
CAAGCGGAAGAGCACACGTCTGAACTCCAGTCACGAGATTCCATCTCGTA150.16602102933038185TruSeq Adapter, Index 7 (97% over 35bp)
GTACGAAAGATTAAAATTGGATCAGTGAATCTTTTTTTAGTCGTTTATTG150.16602102933038185No Hit
GGATATAATATCTTCCACCTCCTTAAACATAAAAATCTAGATACTCTAAT140.1549529607083564No Hit
CTTTATAATCCCAGATTGATATCCTGAACAGCCCTTCTCAGATTGTCAGC140.1549529607083564No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGAGATTCCAGCTCGTA140.1549529607083564TruSeq Adapter, Index 7 (97% over 38bp)
CGACTTAATATATGAGTCTTTGTCTTTGTATCTTCGAACAAGGAAACATT140.1549529607083564No Hit
GCGAATAATCAAACCACATTTATCTCAAGATTATATATTAGGGGTGAAAC140.1549529607083564No Hit
ATATATAAGACCTGATTTTTTTCTATATATATAAGACCTGTTATTTTCTT140.1549529607083564No Hit
TTTCTCCATCTGATCAAACCTTCCGACCCTACCGTACCCACTTAAAACTA130.14388489208633093No Hit
AGAATTAATTATACTTTTTTTTTTCTGGTTTTGTTTTTGTTATTTCTGTT130.14388489208633093No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG130.14388489208633093No Hit
TATCTAGTTACTTTTTCAAAAAAATCCGTGATTTTATCTGATTTTTCAAT130.14388489208633093No Hit
AGTATAACAACTTAAACCGCAACCCAATCTTAAAAGCCTAAGTAGTGTTT130.14388489208633093No Hit
CTACACCAAACCAGCAACTTGTTATATCTCCTCCAGTTGATAATGAGCTA120.13281682346430548No Hit
TTATGACTCCATAACTTTTAGACCGTGAGGCCAAACTTGGCATGTGATAC120.13281682346430548No Hit
AAATAAATAAAGGTGAAACACCCATTTCAAAGAAATGGTAACGTGTCTTT120.13281682346430548No Hit
GTAATGCGAGTCTGGCCATTGGCGAAGTTGTGGAGAAGGAAGATGCAGAG120.13281682346430548No Hit
TTTGTGATGTTAACAAGGGAGTTGGATTTTTTATGGCAGGTCGTGGAGGA120.13281682346430548No Hit
AAATGATTTCTGTAATTATCTAAAATAATTAAAAGCTATAGTTTATTAAA120.13281682346430548No Hit
ATAACGTCGGTGAACTAGACCACCGGAAACAAACTTTATAACTGATTCAT120.13281682346430548No Hit
TTGGATAATATAAGTTACATTTTCCCGATTGAATGTCATTTCAAAACAAA120.13281682346430548No Hit
ATGTTTCCGATAGTAAAAATCAGGAATCACGTGCTTTGGGTAAAGAAATG120.13281682346430548No Hit
ATAAGTTGAGTAATATAATACAGTTTTAATACTTACAATGAAATTTTTTT120.13281682346430548No Hit
CTTAGTAATACATCTCCTAAGGTTAAATCAAGTGACATAAGATTCCAAAA120.13281682346430548No Hit
TTTAAGTTTTGAATGAGTAAAACTTTCTTAAATTAGATTTTTTCAAAAAA110.12174875484228002No Hit
TAAACATATTCAAAAATAACCAAAAATATCCGAAAATATCTAAAAATAAT110.12174875484228002No Hit
TGGAATGGAATAATCCATGGAGTCGAATGCAATCATCATCGAATGGAATC110.12174875484228002No Hit
GGAAATAAACATTTTGGAATTGTTTGATCCATTGATTATTGCCTAGAAGA110.12174875484228002No Hit
CGTACGGAAGAGCACACGTCTGAACTCCAGTCACGAGATTCCATCTCGTA110.12174875484228002TruSeq Adapter, Index 7 (97% over 35bp)
AATGGTATTTCATTATATGATATCAAACTTAAAATATCTAAATAAATATG110.12174875484228002No Hit
TATTGTACAAGAACAATCTCTAGATTGAAGAGACGAAATGTAGGAGAGAC110.12174875484228002No Hit
GAATACGTGTTTCTCCTCTCATTGGCCCTAGAATGGCACCAAATGCTCTC110.12174875484228002No Hit
CCCCTCCTGCGGATTAGTCGACACTTTTCACAAATTTTACGAACGGAAGC110.12174875484228002No Hit
AGTTGTAATCTTTGTATATCCACTCACCAACCATAATTAATACAGGTAAC110.12174875484228002No Hit
CTTTGAGAAGCTAGAAGAAGGTTGGTTAGTGTTTTGGAGTCGAGTATGAC110.12174875484228002No Hit
ACCATCGAAGTATTTTGCAATCTTCTGCTATACTTTAGTTTTCCGAGAAC110.12174875484228002No Hit
CGTAGGAAGAGCACACGTCTGAACTCCAGTCACGAGATTCCATCTCGTAT110.12174875484228002TruSeq Adapter, Index 9 (96% over 33bp)
GATGGTAATCTGGAGAAGCATTCTATCGAGAAATGTTGAATCTCCCATCT110.12174875484228002No Hit
CTAGCTTATGCTCTGACCCGAGTAGCATGGGGCACGTGGAATCCCGTGTG110.12174875484228002No Hit
CGAAGAGCACACGTCTGAACTCCAGTCACGAGATTCCATCTCGTATGCCG110.12174875484228002TruSeq Adapter, Index 9 (96% over 31bp)
ATCTAATACCATAGGATATACAATATATGCTGACGTGACGAATAAATAAA110.12174875484228002No Hit
GAATAAGACTTGATGTCATGTGTATGATTGAGTATAAGAACTTAAACCGC110.12174875484228002No Hit
AGACGTAATTTGTTTATTTCTTTCATATCAAAATGGCATATATGTCAATG110.12174875484228002No Hit
GGCAAGAGCCAGGCCTCGATGAGTAGGAGGGCGCGGCGGTCGCTGCAAAA110.12174875484228002No Hit
AGCATGCAGTGAGAAACAGGGGATCTTAATTTACGCATCACGATCTTTTT110.12174875484228002No Hit
GAGATTAATAAAATCTTTTTCTGCAGATATTTTCTTCGCCAGAGCCTGCT110.12174875484228002No Hit
GAAGTTAAGCTCAGCTTATGCTCAACAAAGAGACTAAGTAAATTCCGTGG100.11068068622025456No Hit
TGCTATTGATGACGACACAGATGAGACAATGTTAGTCGCTTCTTTGGTAG100.11068068622025456No Hit
ATTACACAAGAGACTGTGTAATTTAAGTTTGGGGGAGGGTTCAAGACGTA100.11068068622025456No Hit
GTGATTACATCTTTTTCTCATGCAGTTACAAATAATTAATCAATGTGACT100.11068068622025456No Hit
GAAAACATTATCCAAAAAGCGGAGCTTTACAAAGAAGCACCTTGCTCAGG100.11068068622025456No Hit
AAGCTATTGCGAACAATGAAGCTTTCATTACTCTGAGGAAGATTGAAGCT100.11068068622025456No Hit
AATGATACGGCGACCACCGGAAGAGCACACGTCTGAACTCCAGTCACGAG100.11068068622025456TruSeq Adapter, Index 7 (96% over 33bp)
TATTATTTCCTTTTATCCTGAATATGATCTTCATTATATAATATAGTTTT100.11068068622025456No Hit
AATCATAAACTTTATTGAGATAGGTAAACCAAGGTTACTCCCCCTCCAAG100.11068068622025456No Hit
AGCAAGCCACTGCTACATCCATTTCATTAGGAATTGATGATCTTTTAACG100.11068068622025456No Hit
AATATGGAATTCAAAGGGATCTAATAGGAAATGATACTCTGAATCATAGA100.11068068622025456No Hit
TAGTTGTTATTCACGCGCTTTGTTCTTATTAAAATAAAAAACCGTTGGAT100.11068068622025456No Hit
GACCAGTTCGCCATCTCCCCAACCTTCATACCCACCAAACTTATCACCAT100.11068068622025456No Hit
AAGATTGATAGACTTTAAGAAGCTTTAGGGTTAAGCAACCTGGGTTACAA100.11068068622025456No Hit
TAGAGTAATATCCAAAAGAGAAAATATCTAAAACCTTAAGATTGTTTTCC100.11068068622025456No Hit
CATTCCATCTCCACCAGCTTGGCTGCAAAAAGGACTCACAGCAGCAAAAG100.11068068622025456No Hit
GTGCTCAATTTCATATGTGGCAATTCCACCAAGATCTCTTCGTTAGTTGG100.11068068622025456No Hit
CGTATGCGGAAGAGCACACGTCTGAACTCCAGTCACGAGATTCCATCTCG100.11068068622025456TruSeq Adapter, Index 9 (96% over 33bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGACTGT401.10958354E-1070.000017
GGACTAG207.509685E-570.000017
CGGACTG453.6379788E-1270.000016
CGGACTA207.509685E-570.000016
CTCTATT207.509685E-570.000012
ACTAGTC207.509685E-570.000019
CTGTAGA401.10958354E-1070.0000110
GACTAGT207.509685E-570.000018
AGGGGTG150.002153673470.040
CCGGACT252.6185571E-670.05
TCAAGAT150.002153673470.025
ATTAGGG150.002153673470.037
ACCACAT150.002153673470.013
TACGGAC150.002153673470.04
GCGAATA150.002153673470.01
GAAGATC353.1886884E-970.015
ACTCTAT500.070.01
TCGGACT150.002153673470.05
TAGTCCG252.6185571E-670.011
ATAATCA150.002153673470.05
Produced by FastQC (version 0.11.5)