FastQCFastQC Report
Thu 19 Sep 2019
HT5VLAFXY_n01_13YCol_14YC24_15YCol_16YC24_lib_k-b.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHT5VLAFXY_n01_13YCol_14YC24_15YCol_16YC24_lib_k-b.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1153860
Sequences flagged as poor quality0
Sequence length76
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ACTCTATAGAGTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGAGA951328.244674397240566TruSeq Adapter, Index 7 (97% over 37bp)
ACTCTATTGAGTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGAGA544444.718423378919453TruSeq Adapter, Index 7 (97% over 37bp)
ACCGCATCGGAAGAGCACACGTCTGAACTCCAGTCACGAGATTCCATCTC104680.9072157800773059TruSeq Adapter, Index 9 (97% over 35bp)
ACTCTATCGGAAGAGCACACGTCTGAACTCCAGTCACGAGATTCCATCTC79420.6882984070857816TruSeq Adapter, Index 9 (97% over 35bp)
ACTCTATTAGAGTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGAG77010.6674119910561074TruSeq Adapter, Index 7 (97% over 36bp)
ACTCTATAGAGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGAGAT54120.46903437158754097TruSeq Adapter, Index 7 (97% over 38bp)
GTGAGGTCGGAAGAGCACACGTCTGAACTCCAGTCACGAGATTCCATCTC40870.35420241623767185TruSeq Adapter, Index 9 (97% over 35bp)
GGATCGATCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGAGATT40170.3481358223701316TruSeq Adapter, Index 7 (97% over 38bp)
ACTCTATAGATAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGAGAT27850.24136377030142303TruSeq Adapter, Index 7 (97% over 38bp)
GGATCGGAAGAGCACACGTCTGAACTCCAGTCACGAGATTCCATCTCGTA17180.14889154663477372TruSeq Adapter, Index 7 (97% over 38bp)
ACTCTAATAGAGTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGAG15480.13415839009931882TruSeq Adapter, Index 7 (97% over 36bp)
GTGAGGTGACTGGAGTTCAGACCTCACAGATCGGAAGAGCACACGTCTGA14300.12393184615117953Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
ACTCTATATAGAGTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGA13740.11907857105714732TruSeq Adapter, Index 7 (97% over 35bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACTCTAT580850.069.622281
GGATCGT165400.069.5029451
CCGCATA73400.069.2835852
ACCGCAT205050.068.956371
GTGAGGT159750.068.870491
CTCTATA257900.068.858872
CCGCATT40650.068.1046452
CTCTATT166200.067.956152
GATCGTA65650.067.706412
TCTATAG151900.067.6486363
CCGCATG37950.067.508752
CTCTATC97550.067.200312
GCATCGG17400.067.1827854
GATCGTC36750.067.04652
CGCATAT24100.066.949093
CGCATAA23750.066.9041443
TGAGGTA65350.066.892532
CGCATAG17850.066.8616263
CTATAGA134700.066.828884
CTCTATG76200.066.737732
Produced by FastQC (version 0.11.5)