FastQCFastQC Report
Thu 6 Feb 2025
HT5N7DRX5_n02_wy_flu1_10X.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHT5N7DRX5_n02_wy_flu1_10X.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences820509729
Sequences flagged as poor quality0
Sequence length90
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACATGGGCAGTTAAAAAGCTCGTAGTT53545420.6525872650560612Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGTTGACCTCAAATCAGGTAGG29468080.35914357817443987Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGGTTGAACTTTGGGCCCGGTT15071290.1836820389487423Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGGTTAAAAAGCTCGTAGTTGA14449950.17610942916680516Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGGAGTGAAGCGGCAAAAGCTC13072230.15931840340189313Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGGTTGTTGCAGTTAAAAAGCT10657530.1298891362688522Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGAAAAAAAAAAAAAAAAAAAA9634650.11742273929819547Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GGGCAACTTTGGGGCCGTTCCTTGTCTATGTTCCTTGGAACAGGACGTCA9580160.11675863992101428No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGTTTGACCTCAAATCAGGTA9543360.11631013823115802Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GGCAACTTTGGGGCCGTTCCTTGTCTATGTTCCTTGGAACAGGACGTCAT8452090.10301023499503198No Hit
GCAGTGGTATCAACGCAGAGTACATGGGCAGTTAAAAAGCTCGTAGTTGA8322080.10142573214997186Clontech SMARTer II A Oligonucleotide (100% over 23bp)
CAAATTTGAAATCTGGTACCTTCGGTGCCCGAGTTGTAATTTGGAGAGGG8240980.10043732217585928No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)