FastQCFastQC Report
Sat 14 May 2022
HT5MLBGXL_n02_New_W17_1411_d12_rep2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHT5MLBGXL_n02_New_W17_1411_d12_rep2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1748137
Sequences flagged as poor quality0
Sequence length151
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGCAAAAGCAGGACGGATAAGCAAGAGTAGGCTGGAGCAAACGCGCCAG60630.3468263642952469No Hit
GGTCATAGTGATCCGGTGGTTCTGAATGGAAGGGCCATCGCTCAACGGAT51110.29236838989163894No Hit
GGGTATGGCTGTTCGCCATTTAAAGTGGTACGCGAGCTGGGTTTAGAACG49350.28230052907752656No Hit
GTCTTCCGACTGGGCCTTTCGCTTAATTTGATGCCTGGCAGTTCCCTACT46920.26840001670349634No Hit
GTTCATTAGTACCGGTTAGCTCAACGCATCGCTGCGCTTACACACCCGGC38260.21886156519769331No Hit
GTATCAGGCTGAAAATCTTCTCTCATCCGCCAAAACATCTTCGGCGTTGT35820.2049038490690375No Hit
GATTTAATCTGTATCAGGCTGAAAATCTTCTCTCATCCGCCAAAACATCT35470.20290171765714013No Hit
GATAAAAGGTACTCCGGGGATAACAGGCTGATACCGCCCAAGAGTTCATA33770.19317707937078157No Hit
GGCTGATACCGCCCAAGAGTTCATATCGACGGCGGTGTTTGGCACCTCGA32630.18665585134345877No Hit
GAGCAAAAGCAGGTCATAGTGATCCGGTGGTTCTGAATGGAAGGGCCATC29600.16932311369189026No Hit
ATTTAAAGTGGTACGCGAGCTGGGTTTAGAACGTCGTGAGACAGTTCGGT29470.16857946488175699No Hit
CGCATGGGGAGACCCCACACTACCATCGGCGCTACGGCGTTTCACTTCTG27590.15782515901213692No Hit
GCAAAAGCAGGACGGATAAGCAAGAGTAGGCTGGAGCAAACGCGCCAGCG27480.1571959177112549No Hit
CATTAGTACCGGTTAGCTCAACGCATCGCTGCGCTTACACACCCGGCCTA27450.157024306447378No Hit
TCGTGAGACAGTTCGGTCCCTATCTGCCGTGGGCGCTGGAGAACTGAGGG26930.15404971120684477No Hit
GTTTAGAACGTCGTGAGACAGTTCGGTCCCTATCTGCCGTGGGCGCTGGA25700.14701364938789124No Hit
GTCCCAAGGGTATGGCTGTTCGCCATTTAAAGTGGTACGCGAGCTGGGTT24890.1423801452632145No Hit
GTTCTGAATGGAAGGGCCATCGCTCAACGGATAAAAGGTACTCCGGGGAT22230.12716394653279464No Hit
CATCACTGGTGTTCGGGTTGTCATGCCAATGGCACTGCCCGGTAGCTAAA20640.11806854954731809No Hit
TCCCTACTCTCGCATGGGGAGACCCCACACTACCATCGGCGCTACGGCGT20570.11766812326493863No Hit
GCATTTATTCCGCACCAACGCAAAAAAGCCCAGTCTTCCGACTGGGCCTT20420.11681006694555403No Hit
GAGTAGGCTGGAGCAAACGCGCCAGCGTCGCATCCGGCATTTATTCCGCA20360.11646684441780021No Hit
ACCATCGGCGCTACGGCGTTTCACTTCTGAGTTCGGCATGGGGTCAGGTG19980.11429310174202595No Hit
AGCAAGAGTAGGCTGGAGCAAACGCGCCAGCGTCGCATCCGGCATTTATT19540.11177613653849784No Hit
GGTGGGACCACCGCGCTAAGGCCGCCAGGCAAATTCTGTTTATCAGACCG19410.11103248772836455No Hit
AGCGAAAGCAGGACGGATAAGCAAGAGTAGGCTGGAGCAAACGCGCCAGC19130.10943078259884666No Hit
GGATAAGCAAGAGTAGGCTGGAGCAAACGCGCCAGCGTCGCATCCGGCAT18690.10691381739531856No Hit
ATTTGATGCCTGGCAGTTCCCTACTCTCGCATGGGGAGACCCCACACTAC18560.10617016858518524No Hit
GAATGGAAGGGCCATCGCTCAACGGATAAAAGGTACTCCGGGGATAACAG17890.10233751702526747No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)