FastQCFastQC Report
Sat 14 May 2022
HT5MLBGXL_n02_New_Sp19_2254_d12_rep2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHT5MLBGXL_n02_New_Sp19_2254_d12_rep2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2912996
Sequences flagged as poor quality0
Sequence length151
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATTAATTGATATGCAAAAAACCCCGGCCTTATTGACCGGGGTTTTTCT338011.1603517478225167No Hit
GAGCAAAAGCAGGACGGATAAGCAAAAGTAGGCTGGATAAGACGCGCCAG286700.9842100710059334No Hit
GGGTATGGCTGTTCGCCATTTAAAGTGGTACGCGAGCTGGGTTTAGAACG252110.8654663446156465No Hit
GGTCATAGTGATCCGGTGGTTCTGAATGGAAGGGCCATCGCTCAACGGAT252060.8652947000270512No Hit
GGATAAGACGCGCCAGCGTCGCATCCGGTATTAATTGATATGCAAAAAAC205480.7053906012915911No Hit
GGCTGATACCGCCCAAGAGTTCATATCGACGGCGGTGTTTGGCACCTCGA194030.6660839905032482No Hit
GTTCATTAGTACCGGTTAGCTCAACGCATCGCTGCGCTTACACACCCGGC178800.6138010488170942No Hit
GAGCAAAAGCAGGTCATAGTGATCCGGTGGTTCTGAATGGAAGGGCCATC172160.5910066474516271No Hit
GTATCAGGCTGAAAATCTTCTCTCATCCGCCAAAACATCTTCGGCGTTGT169830.5830080096230822No Hit
TCGTGAGACAGTTCGGTCCCTATCTGCCGTGGGCGCTGGAGAACTGAGGG154220.5294205690636032No Hit
GATAAAAGGTACTCCGGGGATAACAGGCTGATACCGCCCAAGAGTTCATA150790.5176457502859599No Hit
CATTAGTACCGGTTAGCTCAACGCATCGCTGCGCTTACACACCCGGCCTA150750.5175084346150836No Hit
CGCATGGGGAGACCCCACACTACCATCGGCGCTACGGCGTTTCACTTCTG144920.4974946755848618No Hit
GTTTAGAACGTCGTGAGACAGTTCGGTCCCTATCTGCCGTGGGCGCTGGA129610.4449371025569551No Hit
AATCTAATCTGTATCAGGCTGAAAATCTTCTCTCATCCGCCAAAACATCT129030.44294602532924865No Hit
GTCCCAAGGGTATGGCTGTTCGCCATTTAAAGTGGTACGCGAGCTGGGTT128760.4420191445508336No Hit
GCAAAAGCAGGACGGATAAGCAAAAGTAGGCTGGATAAGACGCGCCAGCG126580.4345354404880748No Hit
GCCTTATTGACCGGGGTTTTTCTAATTTGATGCCTGGCAGTTCCCTACTC123590.4242710940900708No Hit
ATTTAAAGTGGTACGCGAGCTGGGTTTAGAACGTCGTGAGACAGTTCGGT121990.41877846725501855No Hit
TCATTAACCCGCTTGTCGCCGGTTAATCTAATCTGTATCAGGCTGAAAAT120910.41507094414135826No Hit
AATCTGTATCAGGCTGAAAATCTTCTCTCATCCGCCAAAACATCTTCGGC120050.4121186572175176No Hit
GGTGGGACCACCGCGCTAAGGCCGCCAGGCAAATTCTGTTTCATTAACCC107980.37068365353059185No Hit
TCCCTACTCTCGCATGGGGAGACCCCACACTACCATCGGCGCTACGGCGT107030.3674224063472796No Hit
GTTCTGAATGGAAGGGCCATCGCTCAACGGATAAAAGGTACTCCGGGGAT104990.3604193071325879No Hit
CATCACTGGTGTTCGGGTTGTCATGCCAATGGCACTGCCCGGTAGCTAAA104670.35932078176557747No Hit
GGGTCAGGTGGGACCACCGCGCTAAGGCCGCCAGGCAAATTCTGTTTCAT104100.3573640334555901No Hit
ACCATCGGCGCTACGGCGTTTCACTTCTGAGTTCGGCATGGGGTCAGGTG100670.3455892146779467No Hit
TCACTGGTGTTCGGGTTGTCATGCCAATGGCACTGCCCGGTAGCTAAATG90410.31036774509817383No Hit
TCCTGGGGCTGAAGTAGGTCCCAAGGGTATGGCTGTTCGCCATTTAAAGT90110.3093378775666015No Hit
GACTTAGAGTCTCCTGAAGGAACGTTGAAGACGACGACGTTGATAGGCCG89540.30738112925661415No Hit
CGCTAAGGCCGCCAGGCAAATTCTGTTTCATTAACCCGCTTGTCGCCGGT89190.3061796171364465No Hit
GGTTAAGCCTCACGGTTCATTAGTACCGGTTAGCTCAACGCATCGCTGCG86200.2959152707384425No Hit
AGCGAAAGCAGGTCATAGTGATCCGGTGGTTCTGAATGGAAGGGCCATCG85000.29179580061215327No Hit
GAATGGAAGGGCCATCGCTCAACGGATAAAAGGTACTCCGGGGATAACAG84850.2912808668463671No Hit
GTCCCTATCTGCCGTGGGCGCTGGAGAACTGAGGGGGGCTGCTCCTAGTA82630.28365984711273207No Hit
ATCTAATCTGTATCAGGCTGAAAATCTTCTCTCATCCGCCAAAACATCTT82470.2831105844292268No Hit
AGCGAAAGCAGGACGGATAAGCAAAAGTAGGCTGGATAAGACGCGCCAGC79250.2720566729236841No Hit
CTCTAAGTCTCAGGGAGAACTCATCTCGGGGCAAGTTTCGTGCTTAGATG78210.26848646548090005No Hit
GTTTGGCACCTCGATGTCGGCTCATCACATCCTGGGGCTGAAGTAGGTCC77580.26632374366459827No Hit
TTGTAAGGTTAAGCCTCACGGTTCATTAGTACCGGTTAGCTCAACGCATC77210.2650535737089924No Hit
AAGCAGGACGGATAAGCAAAAGTAGGCTGGATAAGACGCGCCAGCGTCGC77070.26457296886092535No Hit
TAGTACGAGAGGACCGGAGTGGACGCATCACTGGTGTTCGGGTTGTCATG74900.25712359371588567No Hit
AGCAAAAGTAGGCTGGATAAGACGCGCCAGCGTCGCATCCGGTATTAATT73940.2538280176148543No Hit
GTGCTGAAAGCATCTAAGCACGAAACTTGCCCCGAGATGAGTTCTCCCTG70890.24335769771053584No Hit
GCAAAAGCAGGTCATAGTGATCCGGTGGTTCTGAATGGAAGGGCCATCGC69980.24023376619809983No Hit
CATCACATCCTGGGGCTGAAGTAGGTCCCAAGGGTATGGCTGTTCGCCAT69370.23813970221723615No Hit
GAAGTAGGTCCCAAGGGTATGGCTGTTCGCCATTTAAAGTGGTACGCGAG68820.23625161174268694No Hit
CCTAGTACGAGAGGACCGGAGTGGACGCATCACTGGTGTTCGGGTTGTCA67750.23257841754674569No Hit
GCAAAAAACCCCGGCCTTATTGACCGGGGTTTTTCTAATTTGATGCCTGG67260.2308963005785109No Hit
GTTCCCTACTCTCGCATGGGGAGACCCCACACTACCATCGGCGCTACGGC67160.23055301140132017No Hit
GGGTTTAGAACGTCGTGAGACAGTTCGGTCCCTATCTGCCGTGGGCGCTG66680.22890522335080446No Hit
GTTCGGTCCCTATCTGCCGTGGGCGCTGGAGAACTGAGGGGGGCTGCTCC66540.22842461850273738No Hit
CCCTGAGACTTAGAGTCTCCTGAAGGAACGTTGAAGACGACGACGTTGAT66440.22808132932554664No Hit
CCACACTACCATCGGCGCTACGGCGTTTCACTTCTGAGTTCGGCATGGGG66390.22790968473695122No Hit
ATAGTGATCCGGTGGTTCTGAATGGAAGGGCCATCGCTCAACGGATAAAA66310.22763505339519863No Hit
ACCCCACACTACCATCGGCGCTACGGCGTTTCACTTCTGAGTTCGGCATG66190.22722310638256968No Hit
AAAGTAGGCTGGATAAGACGCGCCAGCGTCGCATCCGGTATTAATTGATA66090.22687981720537895No Hit
TATCTGCCGTGGGCGCTGGAGAACTGAGGGGGGCTGCTCCTAGTACGAGA66060.22677683045222172No Hit
GATAAGTGCTGAAAGCATCTAAGCACGAAACTTGCCCCGAGATGAGTTCT64830.22255437357277524No Hit
GCCCGGTAGCTAAATGCGGAAGAGATAAGTGCTGAAAGCATCTAAGCACG63540.21812594318701434No Hit
GGGGATAACAGGCTGATACCGCCCAAGAGTTCATATCGACGGCGGTGTTT63300.21730204916175647No Hit
GTGTTCGGGTTGTCATGCCAATGGCACTGCCCGGTAGCTAAATGCGGAAG62860.2157915767821171No Hit
GCCCAAGAGTTCATATCGACGGCGGTGTTTGGCACCTCGATGTCGGCTCA62160.2133885525417817No Hit
GTACTAATGAACCGTGAGGCTTAACCTTACAACGCCGAAGATGTTTTGGC62000.2128392898582765No Hit
CAATGGCACTGCCCGGTAGCTAAATGCGGAAGAGATAAGTGCTGAAAGCA61700.21180942232670422No Hit
TCGCTCAACGGATAAAAGGTACTCCGGGGATAACAGGCTGATACCGCCCA61650.2116377777381088No Hit
GGTTAATCTAATCTGTATCAGGCTGAAAATCTTCTCTCATCCGCCAAAAC61520.21119150180776083No Hit
CTAATGAACCGTGAGGCTTAACCTTACAACGCCGAAGATGTTTTGGCGGA61450.21095119938372725No Hit
AAGGTACTCCGGGGATAACAGGCTGATACCGCCCAAGAGTTCATATCGAC61270.21033327886478387No Hit
GTACGAGAGGACCGGAGTGGACGCATCACTGGTGTTCGGGTTGTCATGCC60960.20926908241549252No Hit
GAGTGGACGCATCACTGGTGTTCGGGTTGTCATGCCAATGGCACTGCCCG60290.20696904492831436No Hit
CTGGAGAACTGAGGGGGGCTGCTCCTAGTACGAGAGGACCGGAGTGGACG58410.20051520839712791No Hit
GGTTAGCTCAACGCATCGCTGCGCTTACACACCCGGCCTATCAACGTCGT58220.19986295896046546No Hit
GTTGTAAGGTTAAGCCTCACGGTTCATTAGTACCGGTTAGCTCAACGCAT56890.19529721290382823No Hit
CTTACACACCCGGCCTATCAACGTCGTCGTCTTCAACGTTCCTTCAGGAG56320.19334046459384083No Hit
GTCATGCCAATGGCACTGCCCGGTAGCTAAATGCGGAAGAGATAAGTGCT54280.18633736537914916No Hit
GGAGTGGACGCATCACTGGTGTTCGGGTTGTCATGCCAATGGCACTGCCC53660.1842089724805664No Hit
GTGTTTGGCACCTCGATGTCGGCTCATCACATCCTGGGGCTGAAGTAGGT53490.18362538087934208No Hit
GCGCTACGGCGTTTCACTTCTGAGTTCGGCATGGGGTCAGGTGGGACCAC53410.18335074953758948No Hit
CTTCTGAGTTCGGCATGGGGTCAGGTGGGACCACCGCGCTAAGGCCGCCA53370.18321343386671318No Hit
GTACCGGTTAGCTCAACGCATCGCTGCGCTTACACACCCGGCCTATCAAC51410.1764849659937741No Hit
GTTCATATCGACGGCGGTGTTTGGCACCTCGATGTCGGCTCATCACATCC51280.1760386900634261No Hit
GAGGGGGGCTGCTCCTAGTACGAGAGGACCGGAGTGGACGCATCACTGGT51230.17586704547483073No Hit
CTCCTGAAGGAACGTTGAAGACGACGACGTTGATAGGCCGGGTGTGTAAG50660.17391029716484332No Hit
GTCTCAGGGAGAACTCATCTCGGGGCAAGTTTCGTGCTTAGATGCTTTCA50070.1718848910194178No Hit
CCCCACACTACCATCGGCGCTACGGCGTTTCACTTCTGAGTTCGGCATGG50030.17174757534854151No Hit
GGGAGACCCCACACTACCATCGGCGCTACGGCGTTTCACTTCTGAGTTCG49560.17013411621574487No Hit
GGTCAGGTGGGACCACCGCGCTAAGGCCGCCAGGCAAATTCTGTTTCATT48620.16690719795015166No Hit
TAGTGATCCGGTGGTTCTGAATGGAAGGGCCATCGCTCAACGGATAAAAG48530.16659823769067997No Hit
GTGTAAGCGCAGCGATGCGTTGAGCTAACCGGTACTAATGAACCGTGAGG47720.16381759535543475No Hit
CTACCATCGGCGCTACGGCGTTTCACTTCTGAGTTCGGCATGGGGTCAGG47070.16158621570369475No Hit
GCTCAACGCATCGCTGCGCTTACACACCCGGCCTATCAACGTCGTCGTCT46450.15945782280511198No Hit
GGGTTTTTCTAATTTGATGCCTGGCAGTTCCCTACTCTCGCATGGGGAGA45960.15777570583687722No Hit
AGCAAAAGCAGGACGGATAAGCAAAAGTAGGCTGGATAAGACGCGCCAGC45930.15767271908372No Hit
ATCACATCCTGGGGCTGAAGTAGGTCCCAAGGGTATGGCTGTTCGCCATT45320.1555786551028563No Hit
CCCTACTCTCGCATGGGGAGACCCCACACTACCATCGGCGCTACGGCGTT44750.15362190679286894No Hit
GTATGGCTGTTCGCCATTTAAAGTGGTACGCGAGCTGGGTTTAGAACGTC44080.15132186930569078No Hit
GGCCATCGCTCAACGGATAAAAGGTACTCCGGGGATAACAGGCTGATACC43790.15032633069183754No Hit
GCCTATCAACGTCGTCGTCTTCAACGTTCCTTCAGGAGACTCTAAGTCTC42990.14758001727431141No Hit
CACTACCATCGGCGCTACGGCGTTTCACTTCTGAGTTCGGCATGGGGTCA41460.14232769286329264No Hit
TTTCACTTCTGAGTTCGGCATGGGGTCAGGTGGGACCACCGCGCTAAGGC41230.14153812775575386No Hit
TCCCAAGGGTATGGCTGTTCGCCATTTAAAGTGGTACGCGAGCTGGGTTT41220.1415037988380348No Hit
CCTCGATGTCGGCTCATCACATCCTGGGGCTGAAGTAGGTCCCAAGGGTA41010.14078289156593418No Hit
CTTCAGGAGACTCTAAGTCTCAGGGAGAACTCATCTCGGGGCAAGTTTCG40970.14064557589505786No Hit
GGAGAACTGAGGGGGGCTGCTCCTAGTACGAGAGGACCGGAGTGGACGCA40820.14013064212927173No Hit
CAAAAAACCCCGGCCTTATTGACCGGGGTTTTTCTAATTTGATGCCTGGC39750.1364574479333305No Hit
GCCGTGGGCGCTGGAGAACTGAGGGGGGCTGCTCCTAGTACGAGAGGACC39450.1354275804017582No Hit
GGCAAATTCTGTTTCATTAACCCGCTTGTCGCCGGTTAATCTAATCTGTA39450.1354275804017582No Hit
TTATTGACCGGGGTTTTTCTAATTTGATGCCTGGCAGTTCCCTACTCTCG39150.13439771287018587No Hit
ACTCTCGCATGGGGAGACCCCACACTACCATCGGCGCTACGGCGTTTCAC38520.13223499105388406No Hit
GGCTGAAGTAGGTCCCAAGGGTATGGCTGTTCGCCATTTAAAGTGGTACG37510.1287677703642573No Hit
GTGGTACGCGAGCTGGGTTTAGAACGTCGTGAGACAGTTCGGTCCCTATC37380.12832149443390928No Hit
GGGCTGCTCCTAGTACGAGAGGACCGGAGTGGACGCATCACTGGTGTTCG36460.1251632340037542No Hit
GAAGAGATAAGTGCTGAAAGCATCTAAGCACGAAACTTGCCCCGAGATGA36200.12427068214305821No Hit
CTGGTGTTCGGGTTGTCATGCCAATGGCACTGCCCGGTAGCTAAATGCGG35890.12320648569376684No Hit
CTTATTGACCGGGGTTTTTCTAATTTGATGCCTGGCAGTTCCCTACTCTC35560.12207363140903729No Hit
CGCTTACACACCCGGCCTATCAACGTCGTCGTCTTCAACGTTCCTTCAGG35450.12169601331412745No Hit
GATCCGGTGGTTCTGAATGGAAGGGCCATCGCTCAACGGATAAAAGGTAC35430.1216273554786893No Hit
AGACAGTTCGGTCCCTATCTGCCGTGGGCGCTGGAGAACTGAGGGGGGCT35070.12039151444080254No Hit
ATTCTGTTTCATTAACCCGCTTGTCGCCGGTTAATCTAATCTGTATCAGG35010.12018554093448806No Hit
GTAGCTAAATGCGGAAGAGATAAGTGCTGAAAGCATCTAAGCACGAAACT34640.11891537097888222No Hit
ATTAATTGATATGCAAAAAACCCCGGCCTTATTGACCGGGGTTTTTCTAA34640.11891537097888222No Hit
GAGCGAAAGCAGGACGGATAAGCAAAAGTAGGCTGGATAAGACGCGCCAG33790.1159974129727607No Hit
TCCTTCAGGAGACTCTAAGTCTCAGGGAGAACTCATCTCGGGGCAAGTTT33350.11448694059312131No Hit
ATCTGTATCAGGCTGAAAATCTTCTCTCATCCGCCAAAACATCTTCGGCG33150.11380036223873978No Hit
ACTGAGGGGGGCTGCTCCTAGTACGAGAGGACCGGAGTGGACGCATCACT33090.1135943887324253No Hit
CTATCTGCCGTGGGCGCTGGAGAACTGAGGGGGGCTGCTCCTAGTACGAG32900.11294213929576286No Hit
CCATTTAAAGTGGTACGCGAGCTGGGTTTAGAACGTCGTGAGACAGTTCG32810.11263317903629115No Hit
GGTTTAGAACGTCGTGAGACAGTTCGGTCCCTATCTGCCGTGGGCGCTGG32760.11246153444769579No Hit
GACCCCACACTACCATCGGCGCTACGGCGTTTCACTTCTGAGTTCGGCAT32740.11239287661225761No Hit
GCTCATCACATCCTGGGGCTGAAGTAGGTCCCAAGGGTATGGCTGTTCGC32620.1119809295996287No Hit
AGAGATAAGTGCTGAAAGCATCTAAGCACGAAACTTGCCCCGAGATGAGT32610.11194660068190962No Hit
GGCTGAAAATCTTCTCTCATCCGCCAAAACATCTTCGGCGTTGTAAGGTT32190.1105047861377084No Hit
CTATTGCACCGTTCGTGACAGTAAAGATGTGGTAAACACCACTGAGCAGG32010.109886865618765No Hit
TCTCTCATCCGCCAAAACATCTTCGGCGTTGTAAGGTTAAGCCTCACGGT31680.10875401133403548No Hit
GATAAGCAAAAGTAGGCTGGATAAGACGCGCCAGCGTCGCATCCGGTATT31430.10789578839105855No Hit
TAACAGGCTGATACCGCCCAAGAGTTCATATCGACGGCGGTGTTTGGCAC31400.10779280163790134No Hit
GGGGTCAGGTGGGACCACCGCGCTAAGGCCGCCAGGCAAATTCTGTTTCA31370.10768981488474408No Hit
TCTGAGTTCGGCATGGGGTCAGGTGGGACCACCGCGCTAAGGCCGCCAGG31250.10727786787211517No Hit
TTGATAGGCCGGGTGTGTAAGCGCAGCGATGCGTTGAGCTAACCGGTACT31180.10703756544808163No Hit
GCTCCTAGTACGAGAGGACCGGAGTGGACGCATCACTGGTGTTCGGGTTG31140.10690024977720533No Hit
CATCCTGGGGCTGAAGTAGGTCCCAAGGGTATGGCTGTTCGCCATTTAAA30870.10597336899879026No Hit
AAGCAGGTCATAGTGATCCGGTGGTTCTGAATGGAAGGGCCATCGCTCAA30520.10477185687862256No Hit
GGTATGGCTGTTCGCCATTTAAAGTGGTACGCGAGCTGGGTTTAGAACGT30510.10473752796090348No Hit
CATGGGGAGACCCCACACTACCATCGGCGCTACGGCGTTTCACTTCTGAG30390.10432558094827457No Hit
GCCAATGGCACTGCCCGGTAGCTAAATGCGGAAGAGATAAGTGCTGAAAG30350.10418826527739825No Hit
TACACACCCGGCCTATCAACGTCGTCGTCTTCAACGTTCCTTCAGGAGAC30340.10415393635967918No Hit
GCATGGGGTCAGGTGGGACCACCGCGCTAAGGCCGCCAGGCAAATTCTGT30280.1039479628533647No Hit
CTCTCGCATGGGGAGACCCCACACTACCATCGGCGCTACGGCGTTTCACT30230.10377631826476934No Hit
GCGCTTACACACCCGGCCTATCAACGTCGTCGTCTTCAACGTTCCTTCAG30140.10346735800529765No Hit
ACGCATCGCTGCGCTTACACACCCGGCCTATCAACGTCGTCGTCTTCAAC30090.10329571341670225No Hit
GGACGGATAAGCAAAAGTAGGCTGGATAAGACGCGCCAGCGTCGCATCCG30070.1032270555812641No Hit
GATAACAGGCTGATACCGCCCAAGAGTTCATATCGACGGCGGTGTTTGGC29880.10257480614460164No Hit
CTCCTAGTACGAGAGGACCGGAGTGGACGCATCACTGGTGTTCGGGTTGT29740.10209420129653456No Hit
CGGTTCATTAGTACCGGTTAGCTCAACGCATCGCTGCGCTTACACACCCG29650.10178524103706288No Hit
GAGTAATACAGTGCTATCAAGCTGTTCAAGTGCCATACTGACATACCCTG29300.1005837289168952No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)