FastQCFastQC Report
Sat 14 May 2022
HT5MLBGXL_n02_New_Sp19_2243_d08_rep2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHT5MLBGXL_n02_New_Sp19_2243_d08_rep2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1703585
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTCCAACACTGTGTCAAGCTTTCAGGTAGACTGCTTTCTTTGGCATGTCC129580.7606312570256253No Hit
GAGCAAAAGCAGGGTGACAAAAACATAATGGATTCCAACACTGTGTCAAG69720.4092546013260272No Hit
GTTGTAAGGCTTGCATAAAGGTTATTTGCTCAAAGCTATTCTCTGTAATT69120.4057326168051491No Hit
GTGTTATCATTCCATTCAAGTCCTCCGATGAGGACCCCAATTGCATTTTT44710.26244654654742794No Hit
TTCTTAACCTATGTCGCATTTCTTCAATTAACCATCTTATCTCTTCAAAC44070.25868976305849134No Hit
AAACAAGGGTGTTTTTTATCATTAAATAAGCTGAAACGAGAAAGTTCTTA40260.23632516135091586No Hit
CTATTGCTCCTTCATCTGTAAACGCTCTAAGTAGTATCAGAGCTTCAAGT39780.23350757373421344No Hit
GTGTCAAGCTTTCAGGTAGACTGCTTTCTTTGGCATGTCCGCAAACGATT37230.21853913952048182No Hit
AAGCTATTCTCTGTAATTCTTAACCTATGTCGCATTTCTTCAATTAACCA36810.2160737503558672No Hit
GTTCTTATCTCTTGCTCCACTTCAAGCAATAGTTGTAAGGCTTGCATAAA35720.2096754784762721No Hit
GCATATAGTGGAGCGGATTCTGGAGGAAGAATCTGACGAGGCACTTAAAA35410.20785578647381847No Hit
GTAATGGGTAAAACCATCATATTGAAAGCAAACTTTAGTGTGATTTTTAA35100.20603609447136478No Hit
GCAAAAGCAGGGTGACAAAAACATAATGGATTCCAACACTGTGTCAAGCT34830.20445120143696968No Hit
GGTTAATGCTCATTCCCAAGCAGAAAGTGACAGGGCCCCTTTGCATTAGA34750.20398160350085262No Hit
GCATAAAGGTTATTTGCTCAAAGCTATTCTCTGTAATTCTTAACCTATGT32650.1916546576777795No Hit
CTCTTGGTCTGGACATCAGAACTGCCACTCGTGAAGGAAAGCATATAGTG31580.18537378528221368No Hit
ACCTAACTGAAATGACTCTTGAGGAAATGTCAAGGGATTGGTTAATGCTC31320.1838475919898332No Hit
GTAGAAACAAGGGTGTTTTTTATCATTAAATAAGCTGAAACGAGAAAGTT31270.18355409327976002No Hit
CTGTAATTCTTAACCTATGTCGCATTTCTTCAATTAACCATCTTATCTCT29680.17422083429943325No Hit
GTATAGGTGGGTGGGTGATGTATGCCCCACACGAAAAGAATGCTCTTTCC29070.1706401500365406No Hit
AAGTAGTATCAGAGCTTCAAGTCGATTAAAAATCACACTAAAGTTTGCTT29020.17034665132646742No Hit
AGAATGGACCAGGCAGTAATGGGTAAAACCATCATATTGAAAGCAAACTT29010.17028795158445278No Hit
ACTTAGAGCGTTTACAGATGAAGGAGCAATAGTGGGCGAAATCTCACCAT28560.16764646319379425No Hit
GTCCTGGAAGGGAAGGTAATGGTGAGATTTCGCCCACTATTGCTCCTTCA28280.1660028704173845No Hit
CCCTAAGAGGAAGAGGCAGCACTCTTGGTCTGGACATCAGAACTGCCACT26910.15796100576137967No Hit
GAGCAAAAGCAGGTAGATGTTGAAAGATGAGTCTTCTAACCGAGGTCGAA26150.1534998253682675No Hit
GCTCTATGTTGACAAAATGACCATCGTCAACATCCACAGCATTCTGCTGT23560.13829659218647733No Hit
GTTTTAGTACCGACCAATAATAATCAATTCTTCCCTGCAGACCATTGACA23330.13694649812014076No Hit
CTCTTGCTCCACTTCAAGCAATAGTTGTAAGGCTTGCATAAAGGTTATTT23230.13635950069999442No Hit
ACCCATTACTGCCTGGTCCATTCTAATGCAAAGGGGCCCTGTCACTTTCT22640.13289621592113104No Hit
ATATAGTGGAGCGGATTCTGGAGGAAGAATCTGACGAGGCACTTAAAATG22250.1306069259825603No Hit
GTCCTACATCGTCGAAAGATCATCAGCTGTAAATGGAACGTGTTACCCTG22210.13037212701450177No Hit
GGGTAAAACCATCATATTGAAAGCAAACTTTAGTGTGATTTTTAATCGAC21730.12755453939779934No Hit
ACATAATGGATTCCAACACTGTGTCAAGCTTTCAGGTAGACTGCTTTCTT21580.12667404326757983No Hit
GTATCAGAGCTTCAAGTCGATTAAAAATCACACTAAAGTTTGCTTTCAAT21160.12420865410296522No Hit
GAGTGGAGATCTCCCATTCTCATCACTGCTTCTCCAAGTGAATCTCTGTA20880.12256506132655547No Hit
TTCCTTGACCGGCTTCGCCGAGATCAGAAGTCCCTAAGAGGAAGAGGCAG20110.11804518119142865No Hit
AGCGAAAGCAGGGTGACAAAAACATAATGGATTCCAACACTGTGTCAAGC19990.11734078428725307No Hit
CTTAAAATGACTATCGCTTCAGTGCCTGCTTCACGCTACCTAACTGAAAT19960.11716468506120915No Hit
GAACTGGGTGATGCCCCATTCCTTGACCGGCTTCGCCGAGATCAGAAGTC19500.11446449692853601No Hit
GTTTTTTATCATTAAATAAGCTGAAACGAGAAAGTTCTTATCTCTTGCTC19500.11446449692853601No Hit
CTCATAGACTCAGGCACTCCTTCCGTAGAAGGCCCTCTTTTCAAACCGTA18910.1110012121496726No Hit
GCATTAGAATGGACCAGGCAGTAATGGGTAAAACCATCATATTGAAAGCA18810.11041421472952626No Hit
CTTCAAACTTCTGGCTCAATTGTTCTCTCCACTTTCCGTTTCTGTTTTGG18610.10924021988923359No Hit
CTCCCATTCTCATCACTGCTTCTCCAAGTGAATCTCTGTAGAGTTTCAGA18330.10759662711282383No Hit
ATACTACTTAGAGCGTTTACAGATGAAGGAGCAATAGTGGGCGAAATCTC18280.10730312840275066No Hit
ATATTATTCACCTTGGATGTTATTTTATCAATTGCCTTTTGAGTTGAATC18090.10618783330447262No Hit
AGCAGGTACTGATCCAAAATGGAAGACTTTGTGCGACAGTGCTTCAATCC17770.10430944156000434No Hit
CTGTAGAGTTTCAGAGACTCGAACTGTGTTATCATTCCATTCAAGTCCTC17520.1028419480096385No Hit
CTTCAGTGCCTGCTTCACGCTACCTAACTGAAATGACTCTTGAGGAAATG17180.10084615678114095No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)