FastQCFastQC Report
Sat 14 May 2022
HT5MLBGXL_n02_New_Sm18_1986_d10_rep2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHT5MLBGXL_n02_New_Sm18_1986_d10_rep2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2105594
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTGTAAGGCTTGCATAAAGGTTATTTGCTCAAAGCTATTCTCTGTAATT141630.6726367951276456No Hit
TTCTTAACCTATGTCGCATTTCTTCAATTAACCATCTTATCTCTTCAAAC97840.4646669775844726No Hit
GTTCTTATCTCTTGCTCCACTTCAAGCAATAGTTGTAAGGCTTGCATAAA73030.34683799440917856No Hit
ACCTAACTGAAATGACTCTTGAGGAAATGTCAAGGGATTGGTTAATGCTC71920.34156632285236377No Hit
GGTTAATGCTCATTCCCAAGCAGAAAGTGACAGGGCCCCTTTGCATTAGA67590.3210020545271311No Hit
TTCCAACACTGTGTCAAGCTTTCAGGTAGACTGCTTTCTTTGGCATGTCC67360.3199097261865298No Hit
GCATAAAGGTTATTTGCTCAAAGCTATTCTCTGTAATTCTTAACCTATGT65970.31330826360637426No Hit
AGAATGGACCAGGCAGTAATGGGTAAAACCATCATATTGAAAGCAAACTT62630.29744575639938187No Hit
GCATATAGTGGAGCGGATTCTGGAGGAAGAATCTGACGAGGCACTTAAAA61280.2910342639654178No Hit
AAGCTATTCTCTGTAATTCTTAACCTATGTCGCATTTCTTCAATTAACCA61020.28979945801517293No Hit
CTGTAATTCTTAACCTATGTCGCATTTCTTCAATTAACCATCTTATCTCT59410.282153159630964No Hit
GTAATGGGTAAAACCATCATATTGAAAGCAAACTTTAGTGTGATTTTTAA59410.282153159630964No Hit
GTGTTATCATTCCATTCAAGTCCTCCGATGAGGACCCCAATTGCATTTTT56160.2667180852529025No Hit
TTTTTGGACAGTATGGATAGCAAATAGTAGCATTGCCACAACTATTTTAG54820.26035408535548643No Hit
ACTTAGAGCGTTTACAGATGAAGGAGCAATAGTGGGCGAAATCTCACCAT49700.23603790664297103No Hit
AGCAGGTACTGATCCAAAATGGAAGACTTTGTGCGACAGTGCTTCAATCC49700.23603790664297103No Hit
CTCTTGCTCCACTTCAAGCAATAGTTGTAAGGCTTGCATAAAGGTTATTT48150.22867656347804943No Hit
AAACAAGGGTGTTTTTTATCATTAAATAAGCTGAAACGAGAAAGTTCTTA46480.2207453098745532No Hit
GGGTAAAACCATCATATTGAAAGCAAACTTTAGTGTGATTTTTAATCGAC45870.21784826514513245No Hit
GTATGGATAGCAAATAGTAGCATTGCCACAACTATTTTAGTGCATGTGTG44800.2127665637345091No Hit
ATATAGTGGAGCGGATTCTGGAGGAAGAATCTGACGAGGCACTTAAAATG44090.20939459363960955No Hit
ATTTTGGAGGGAGTGGAGATCTCCCATTCTCATCACTGCTTCTCCAAGTG44010.2090146533472265No Hit
CTTAAAATGACTATCGCTTCAGTGCCTGCTTCACGCTACCTAACTGAAAT41240.1958592207234633No Hit
GCATTAGAATGGACCAGGCAGTAATGGGTAAAACCATCATATTGAAAGCA40410.19191734018998913No Hit
GAGCAAAAGCAGGGTGACAAAAACATAATGGATTCCAACACTGTGTCAAG40050.1902076088742654No Hit
GAGCAAAAGCAGGTACTGATCCAAAATGGAAGACTTTGTGCGACAGTGCT37400.1776220866890768No Hit
CTCTTGGTCTGGACATCAGAACTGCCACTCGTGAAGGAAAGCATATAGTG35820.17011826591451154No Hit
GTAGAAACAAGGGTGTTTTTTATCATTAAATAAGCTGAAACGAGAAAGTT35740.16973832562212848No Hit
ATACTACTTAGAGCGTTTACAGATGAAGGAGCAATAGTGGGCGAAATCTC35450.16836104206223992No Hit
GAGTGGAGATCTCCCATTCTCATCACTGCTTCTCCAAGTGAATCTCTGTA31610.15012390802785341No Hit
CAATAGTTGTAAGGCTTGCATAAAGGTTATTTGCTCAAAGCTATTCTCTG30870.1466094603233102No Hit
CTTCAGTGCCTGCTTCACGCTACCTAACTGAAATGACTCTTGAGGAAATG30220.1435224454476979No Hit
CTCCCATTCTCATCACTGCTTCTCCAAGTGAATCTCTGTAGAGTTTCAGA29590.14053041564518134No Hit
GCTCAAAGCTATTCTCTGTAATTCTTAACCTATGTCGCATTTCTTCAATT27480.13050949043357837No Hit
CTTCAAACTTCTGGCTCAATTGTTCTCTCCACTTTCCGTTTCTATTTTGG26600.1263301472173648No Hit
GGTTATTTGCTCAAAGCTATTCTCTGTAATTCTTAACCTATGTCGCATTT26310.12495286365747622No Hit
GCTTCAATCCAATGATTGTCGAGCTTGCGGAAAAGACAATGAAGGAATAT26070.12381304278032707No Hit
TCTTATCTCTTCAAACTTCTGGCTCAATTGTTCTCTCCACTTTCCGTTTC25600.12158089356257663No Hit
GGATTGGTTAATGCTCATTCCCAAGCAGAAAGTGACAGGGCCCCTTTGCA25590.12153340102602876No Hit
TTCCCAAGCAGAAAGTGACAGGGCCCCTTTGCATTAGAATGGACCAGGCA25330.12029859507578385No Hit
GAAAGTGACAGGGCCCCTTTGCATTAGAATGGACCAGGCAGTAATGGGTA25090.11915877419863467No Hit
ACTTCAAGCAATAGTTGTAAGGCTTGCATAAAGGTTATTTGCTCAAAGCT24640.11702161005398001No Hit
ATTTTAGTGCATGTGTGAGGAAGGAGTTGAACCAAGATGCATTAAGCAAA24610.11687913244433637No Hit
CTATTGCTCCTTCATCTGTAAACGCTCTAAGTAGTATCAGAGCTTCAAGT24430.1160242667864745No Hit
ATCATATTGAAAGCAAACTTTAGTGTGATTTTTAATCGACTTGAAGCTCT24030.11412456532455925No Hit
TCATTAAATAAGCTGAAACGAGAAAGTTCTTATCTCTTGCTCCACTTCAA23800.11303223698395798No Hit
GCAAAAGCAGGGTGACAAAAACATAATGGATTCCAACACTGTGTCAAGCT23460.11141749074133No Hit
CTCTTGAGGAAATGTCAAGGGATTGGTTAATGCTCATTCCCAAGCAGAAA23400.1111325355220427No Hit
CTCTGATACTACTTAGAGCGTTTACAGATGAAGGAGCAATAGTGGGCGAA22240.10562340128248844No Hit
GCTTCACGCTACCTAACTGAAATGACTCTTGAGGAAATGTCAAGGGATTG21960.10429361025914778No Hit
TCTCTGTAGAGTTTCAGAGACTCGAACTGTGTTATCATTCCATTCAAGTC21610.10263137147997192No Hit
CTACCTAACTGAAATGACTCTTGAGGAAATGTCAAGGGATTGGTTAATGC21140.10039922226222149No Hit
GCTCTATGTTGACAAAATGACCATCGTCAACATCCACAGCATTCTGCTGT21120.10030423718912573No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)