FastQCFastQC Report
Sat 14 May 2022
HT5MLBGXL_n02_New_P1_SynRNA_2.5_rep2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHT5MLBGXL_n02_New_P1_SynRNA_2.5_rep2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1837507
Sequences flagged as poor quality0
Sequence length151
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCTTATACAATCCAGCCCTGTTAGTTCTGGATGCTGGACAAAACTCCCG132720.7222829627315706No Hit
TTTTTAAACTATTCGACAATTAATTGATGGCCGCCCGAATTCTTTTGGTC83140.4524608613735893No Hit
TATGTATCCTATCTGATATTCCAGATTCTGGTTGAAAGACACCCACGGTC73090.39776719217940393No Hit
ACTCAGCACCGTCCGGCCAAGACCAACCCACAGTGTCACTGTTTACACCA71370.38840668362079706No Hit
ATTCTACACTGTAGAGACCAATTAGAGCACATCCAGAAACTGATTGCCCC66710.363046236014339No Hit
GGATACATATGTAGTGGGATTTTCGGAGACAATCCACGCCCTAATGATAA65520.35657007020925635No Hit
CTACATATGTATCCTATCTGATATTCCAGATTCTGGTTGAAAGACACCCA57800.3145566248183No Hit
CATATGTAGTGGGATTTTCGGAGACAATCCACGCCCTAATGATAAGACAG56110.3053593809438549No Hit
GTAGATGGATGGGCTATATACAGTAAAGACAACAGTGTAAGAATCGGTTC54440.29627098019218434No Hit
CTATCAAGAGTTGGAGAAACAATATATTGAGAACACAAGAGTCTGAATGT49930.2717268560065349No Hit
ATGTATAGGTTATCATGCGAACAATTCAACAGACACTGTAGACACAGTAC48750.26530511176283955No Hit
GTGATGGACAGGCCTCATACAAGATCTTCAGAATAGAAAAGGGAAAGATA48390.2633459355528986No Hit
GAGCAAAAGCAGGTCAATTATATTCAATATGGAGAGAATAAAAGAACTGA47070.2561622894497817No Hit
GATGTATATTATGAAATGGGAGGCTGGTGTTTATAGCACCCTTGGGTGTC46190.25137319204770375No Hit
GTTATTGTCTGTCCCAGTCCATCCGTTCGGATCCCAAATCATCTCAAAAC45660.24848884929417955No Hit
CAATATGGATTAGCCACTCAATTCAACTTGGGAATCAAAATCAGATTGAA44150.24027119352470497No Hit
ATCCTGACCATTCATTTATTCCTACGATATCTTGCTTTATTGAGAAGTTA42370.2305841555977746No Hit
GAATAATAACAGACACTATCAAGAGTTGGAGAAACAATATATTGAGAACA41910.22808076377396114No Hit
GCTATATCCTGACCATTCATTTATTCCTACGATATCTTGCTTTATTGAGA41140.22389030354714295No Hit
GCTATATACAGTAAAGACAACAGTGTAAGAATCGGTTCCAAGGGGGATGT40860.22236649982829998No Hit
GTCCATCACTTGGTCCATCGGTCATTACAGTAAAGCAAGAACCATTTACA40840.22225765670552547No Hit
ATACTGGACCACAACTGCCTGTCTTATCATTAGGGCGTGGATTGTCTCCG40410.21991752956587376No Hit
ACACTGTAGAGACCAATTAGAGCACATCCAGAAACTGATTGCCCCCAGGG39290.21382231469050184No Hit
GTCCCAGTCCATCCGTTCGGATCCCAAATCATCTCAAAACCGTTCCTTGA39050.21251619721720788No Hit
GAGCAAAAGCAGGGGAAAATAAAAACAACCAAAATGGAGGCAATACTAGT38340.20865226635871317No Hit
CTGTAGACACAGTACTAGAAAAGAATGTAACAGTAACACACTCTGTTAAC37530.20424411988634603No Hit
GAATAAAAGAACTGAGAGATCTAATGTCGCAGTCCCGCACTCGCGAGATA37260.20277473772889026No Hit
GTAGAGACCAATTAGAGCACATCCAGAAACTGATTGCCCCCAGGGAGACT36200.19700605222184187No Hit
GTGTTAAAGTACAACGGAATAATAACAGACACTATCAAGAGTTGGAGAAA35650.19401286634554318No Hit
GTGATAATTAGGGGCATTCATTTCGACTGATTTGACTATCTTTCCCTTTT35250.1918360038900532No Hit
GGCTATATACAGTAAAGACAACAGTGTAAGAATCGGTTCCAAGGGGGATG34560.188080916154333No Hit
GTGTAAGAATCGGTTCCAAGGGGGATGTGTTTGTCATAAGGGAACCATTC34420.18731901429491152No Hit
ATCCAAACACCATTGCCGTATTTGAATGAAAATCCTTTTACTCCATTTGC34290.1866115339968773No Hit
GTAGAAACAAGGTCGTTTTTAAACTATTCGACAATTAATTGATGGCCGCC34230.1862850046285538No Hit
TCTCAAAACCGTTCCTTGAACTAATGCTTTTAGTTCTCCCTATCCAAACA33630.18301971094531885No Hit
GGATTAGCCACTCAATTCAACTTGGGAATCAAAATCAGATTGAAACATGC33190.1806251622442799No Hit
GTAATGACCGATGGACCAAGTGATGGACAGGCCTCATACAAGATCTTCAG32990.17953673101653492No Hit
GTCCTATTGGTGAAGTGCCCTCTCCATACAACTCAAGATTTGAGTCAGTC32950.1793190447709859No Hit
GTATGAGGCCTGTCCATCACTTGGTCCATCGGTCATTACAGTAAAGCAAG32930.17921020164821141No Hit
ATCTACTAGTGCTGACCAACAAAGCCTCTATCAGAATGCAGATGCATATG32710.17801292729769194No Hit
AAAGACAACAGTGTAAGAATCGGTTCCAAGGGGGATGTGTTTGTCATAAG32530.17703333919272143No Hit
GTCTTATCATTAGGGCGTGGATTGTCTCCGAAAATCCCACTACATATGTA32480.1767612313857852No Hit
GTCCAGTATCGTCTAATGGAGCAAATGGAGTAAAAGGATTTTCATTCAAA32330.17594490796497647No Hit
AATTATCACTATGAGGAATGCTCCTGTTATCCTGATTCTAGTGAAATCAC32160.17501974142139323No Hit
ATTATATTCAATATGGAGAGAATAAAAGAACTGAGAGATCTAATGTCGCA31910.173659202386712No Hit
GTCCTACATTGTGGAAACACCTAGTTCAGACAATGGAACGTGTTACCCAG31540.17164560461538378No Hit
ATACAATCCAGCCCTGTTAGTTCTGGATGCTGGACAAAACTCCCGCTATA31150.16952316372128107No Hit
TTCCAATTGTGATCGGATGTATATTATGAAATGGGAGGCTGGTGTTTATA31070.16908779123018308No Hit
ACAATATATTGAGAACACAAGAGTCTGAATGTGCATGTGTAAATGGTTCT30860.16794493844105085No Hit
TCATTAGGGCGTGGATTGTCTCCGAAAATCCCACTACATATGTATCCTAT30500.16598576223110986No Hit
ATATACAGTAAAGACAACAGTGTAAGAATCGGTTCCAAGGGGGATGTGTT30330.16506059568752665No Hit
CCCTAATGATAAGACAGGCAGTTGTGGTCCAGTATCGTCTAATGGAGCAA29930.16288373323203667No Hit
GCATACACATGTGATTTCACTAGAATCAGGATAACAGGAGCATTCCTCAT29150.15863885144383122No Hit
TACTAGTGCTGACCAACAAAGCCTCTATCAGAATGCAGATGCATATGTTT28840.1569517830408265No Hit
GCTATATACATTTGCAACCGCAAATGCAGACACATTATGTATAGGTTATC28470.1549381852694983No Hit
CTAATGAGCTGTCCTATTGGTGAAGTGCCCTCTCCATACAACTCAAGATT28130.1530878521823318No Hit
GTGTAAATGGTTCTTGCTTTACTGTAATGACCGATGGACCAAGTGATGGA27640.1504211956743566No Hit
ATCAAGAGTTGGAGAAACAATATATTGAGAACACAAGAGTCTGAATGTGC27310.1486252841485774No Hit
TGATAAGACAGGCAGTTGTGGTCCAGTATCGTCTAATGGAGCAAATGGAG27040.14715590199112166No Hit
GATTACTTGTCAATGGTAAATGGCAACTCAGCACCGTCCGGCCAAGACCA26700.14530556890395518No Hit
ATCCAGCCCTGTTAGTTCTGGATGCTGGACAAAACTCCCGCTATATCCTG26500.14421713767621022No Hit
ATTAAAGACAGGAGCCCATATCGAACCCTAATGAGCTGTCCTATTGGTGA26420.1437817651851122No Hit
AAACAAGGTCGTTTTTAAACTATTCGACAATTAATTGATGGCCGCCCGAA26140.14225796146626923No Hit
GTTCACAGCAGTAGGTAAAGAGTTCAACCACCTGGAAAAAAGAATAGAGA26080.14193143209794576No Hit
CATTTATTCCTACGATATCTTGCTTTATTGAGAAGTTATTGTCTGTCCCA25970.14133279492268602No Hit
GAGCAAAAGCAGGGGTTTAAAATGAATCCAAACCAAAAGATAATAACCAT25730.140026677449392No Hit
CTCTTGATAGTGTCTGTTATTATTCCGTTGTACTTTAACACAGCCACTGC25540.1389926677830343No Hit
GTTAAAGTACAACGGAATAATAACAGACACTATCAAGAGTTGGAGAAACA25530.13893824622164705No Hit
CTCCATACAACTCAAGATTTGAGTCAGTCGCTTGGTCAGCAAGTGCTTGT25110.1366525406433826No Hit
GTCTGAATGTGCATGTGTAAATGGTTCTTGCTTTACTGTAATGACCGATG24940.13572737409979935No Hit
CCTCAATCCTGTGGCCAGTCTCAATTTTGTGCTTTTTACATATTTTGGAC24840.13518315848592685No Hit
CCATTAGACGATACTGGACCACAACTGCCTGTCTTATCATTAGGGCGTGG24830.1351287369245396No Hit
GTGTCACTGTTTACACCACAAAAAGATATGCTGCTCCCGCTAGTCCAGAT24140.1313736491888194No Hit
TCTCAATATGGATTAGCCACTCAATTCAACTTGGGAATCAAAATCAGATT24050.1308838551363342No Hit
TTATTATTCCGTTGTACTTTAACACAGCCACTGCCCCATTGTCTGGGCCA23860.12984984546997644No Hit
AATTAGGGGCATTCATTTCGACTGATTTGACTATCTTTCCCTTTTCTATT23780.12941447297887845No Hit
GATTAGTTCAACCCAGAAGCAAGGTCTTATACAATCCAGCCCTGTTAGTT23740.12919678673332946No Hit
GTCTAATGGAGCAAATGGAGTAAAAGGATTTTCATTCAAATACGGCAATG23660.12876141424223148No Hit
TCTCCATACAACTCAAGATTTGAGTCAGTCGCTTGGTCAGCAAGTGCTTG23600.12843488487390797No Hit
CATTATGTATAGGTTATCATGCGAACAATTCAACAGACACTGTAGACACA23590.1283804633125207No Hit
ATAACAGACACTATCAAGAGTTGGAGAAACAATATATTGAGAACACAAGA23540.12810835550558447No Hit
CAATATATTGAGAACACAAGAGTCTGAATGTGCATGTGTAAATGGTTCTT23320.126911081155065No Hit
GGATAACAGGAGCATTCCTCATAGTGATAATTAGGGGCATTCATTTCGAC22900.12462537557680052No Hit
CACTAAGACCACTGTGGACCATATGGCCATAATCAAAAAGTACACATCAG22860.12440768933125153No Hit
TCCTCATAGTGATAATTAGGGGCATTCATTTCGACTGATTTGACTATCTT22130.12043491534998235No Hit
GTCTTTAATGGTTCCATTGGAATGTTTGTCATTTAGCAAGGCCCCTTGAG22080.12016280754304609No Hit
AAGATATGCTGCTCCCGCTAGTCCAGATTGTGTTCTCTTTGGGTCGCCCT22040.1199451212974971No Hit
CCCTTGGAATGCAGAACCTTCTTCTTGACTCAAGGGGCCTTGCTAAATGA21870.11901995475391387No Hit
ATCTATTTCTTCTCTGTTTAATTTTGCTTCCTCTGAGTATTTTGGGTAGT21460.11678867073703664No Hit
TTCCTGGACATTTGGACTTACAATGCCGAACTGTTGGTTCTATTGGAAAA21350.1161900335617769No Hit
GAATGTGCATGTGTAAATGGTTCTTGCTTTACTGTAATGACCGATGGACC21350.1161900335617769No Hit
GTTTACACCACAAAAAGATATGCTGCTCCCGCTAGTCCAGATTGTGTTCT21270.11575466107067892No Hit
ATCTAATGTCGCAGTCCCGCACTCGCGAGATACTCACTAAGACCACTGTG21010.11433970047461044No Hit
GTATGGAGAGGGCACTTCACCAATAGGACAGCTCATTAGGGTTCGATATG20920.11384990642212518No Hit
CCCCTAATTATCACTATGAGGAATGCTCCTGTTATCCTGATTCTAGTGAA20780.1130880045627037No Hit
ATATTCTACACTGTAGAGACCAATTAGAGCACATCCAGAAACTGATTGCC20760.11297916143992921No Hit
GTCATAAGGGAACCATTCATATCATGCTCCCCCTTGGAATGCAGAACCTT20680.11254378894883121No Hit
TTACTGTAATGACCGATGGACCAAGTGATGGACAGGCCTCATACAAGATC20670.11248936738744396No Hit
CTATGAGGAATGCTCCTGTTATCCTGATTCTAGTGAAATCACATGTGTAT20580.11199957333495872No Hit
GCAAAAGCAGGTCAATTATATTCAATATGGAGAGAATAAAAGAACTGAGA20550.11183630865079697No Hit
CTGTTAACCTTCTAGAAGACAAGCATAACGGGAAACTATGCAAACTAAGA20510.11161862240524799No Hit
CCATTTACACATGCACATTCAGACTCTTGTGTTCTCAATATATTGTTTCT20500.11156420084386072No Hit
ATGTTAACATCAGCAACACCAACTTTGCTGCTGGACAGTCAGTGGTTTCC20370.1108567205458265No Hit
CTTTAACACAGCCACTGCCCCATTGTCTGGGCCAGAAATTCCAATTGTTA20170.10976828931808151No Hit
CAATATGGAGAGAATAAAAGAACTGAGAGATCTAATGTCGCAGTCCCGCA20060.10916965214282177No Hit
CAGTAAAGACAACAGTGTAAGAATCGGTTCCAAGGGGGATGTGTTTGTCA20020.10895196589727277No Hit
TTTTTGAACAGATTACTTGTCAATGGTAAATGGCAACTCAGCACCGTCCG19800.1077546915467533No Hit
CATAATCTCAATATGGATTAGCCACTCAATTCAACTTGGGAATCAAAATC19630.10682952500317006No Hit
TACATATTCTACACTGTAGAGACCAATTAGAGCACATCCAGAAACTGATT19600.1066662603190083No Hit
ACATATGTATCCTATCTGATATTCCAGATTCTGGTTGAAAGACACCCACG19450.10584993689819956No Hit
GCCTCATACAAGATCTTCAGAATAGAAAAGGGAAAGATAGTCAAATCAGT19390.10552340752987607No Hit
GTACTAGAAAAGAATGTAACAGTAACACACTCTGTTAACCTTCTAGAAGA19370.10541456440710158No Hit
CCTCATAGTGATAATTAGGGGCATTCATTTCGACTGATTTGACTATCTTT19200.10448939786351834No Hit
CAATGGGGCAGTGGCTGTGTTAAAGTACAACGGAATAATAACAGACACTA19190.10443497630213111No Hit
GAACACAAGAGTCTGAATGTGCATGTGTAAATGGTTCTTGCTTTACTGTA19020.10350980975854787No Hit
TTTCTATTCTGAAGATCTTGTATGAGGCCTGTCCATCACTTGGTCCATCG18950.10312885882883711No Hit
AGTAGAAACAAGGTCGTTTTTAAACTATTCGACAATTAATTGATGGCCGC18890.10280232946051361No Hit
ATATATTGTTTCTCCAACTCTTGATAGTGTCTGTTATTATTCCGTTGTAC18770.10214927072386662No Hit
TGCACATTCAGACTCTTGTGTTCTCAATATATTGTTTCTCCAACTCTTGA18490.10062546700502364No Hit
AGTTATCCCTGCATACACATGTGATTTCACTAGAATCAGGATAACAGGAG18430.10029893763670016No Hit
GTTCCAAGGGGGATGTGTTTGTCATAAGGGAACCATTCATATCATGCTCC18410.10019009451392566No Hit
ATTGAGAAGTTATTGTCTGTCCCAGTCCATCCGTTCGGATCCCAAATCAT18410.10019009451392566No Hit
CATGTGATTTCACTAGAATCAGGATAACAGGAGCATTCCTCATAGTGATA18400.1001356729525384No Hit
GGTGTATCTGAAATGATAATACCAGATCCAGCGTTTCTTTCCATTGCGAA18400.1001356729525384No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)