FastQCFastQC Report
Sat 14 May 2022
HT5MLBGXL_n02_New_FCC_Seq_Neg_rep2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHT5MLBGXL_n02_New_FCC_Seq_Neg_rep2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences33
Sequences flagged as poor quality0
Sequence length151
%GC48

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGCCCATCCTTCGATCCGAAATAATAGGTGACGGCATTGGTGTCGCCACA26.0606060606060606No Hit
CCCTAAGATCAATGATTCTCATATTAAGAGGAAGTGAAGAGTTGGTTTCA13.0303030303030303No Hit
CTCCCAATGGGATGTGGGGAAAAGAGGCCTCAGAGGGACAACGTAACAAG13.0303030303030303No Hit
GCATATCTCGGTACCACTAGATTTCCAGTTGCTTCGAATGTTATTTTGTC13.0303030303030303No Hit
TATTTCGATAGACCATACGTGATACCAGGACCAAGAGAGGAGAGAAAAAG13.0303030303030303No Hit
GTATACAATTCCTCTGAGACCTGATTTGGCAGCCAGTCTGATGTCTGTGG13.0303030303030303No Hit
GGGCCGGAGTTAGGGTATGCTCCTTGGCCTGGGTATTGGTAGCCTTGTCA13.0303030303030303No Hit
TCAATGAAGCCGGCAATGGCCCCGAATAGGCCTCTAGATTGAATAGACGG13.0303030303030303No Hit
CATTCAGGTTGGAATGCCAAATCATGATATGAGTAAGACCTGCTGTTGCA13.0303030303030303No Hit
GACTGAGAGAGCATAATGACTGGGTCGGCCGATCTCTTTAAAAAGGATTT13.0303030303030303No Hit
GGATGATAGAAATGCGACTGCTAGCATCATTTATGATGGGATGCTTACCG13.0303030303030303No Hit
GAACCGACGAACTCGCGTGACTTCCGGTAAAGGCTATCTGCCAAGGGGTG13.0303030303030303No Hit
TTAGTCTACATTGATGGTTAGTTACTATATGTCTTTGCATGCATCAACTG13.0303030303030303No Hit
CTTCTTTCTTAATCCTTCCAGACTCGAAGTCAATCCGTGCATCAATTCGG13.0303030303030303No Hit
AGTAGAAACAAGGGGAAGGGGAAGGCAGTAAGGTAAAGCACTCAAAGAAA13.0303030303030303No Hit
CCACAGGCTGAGGCAGGAGGATAGCTTAAGCCCAGGAGTTCAAGGCTGCA13.0303030303030303No Hit
TGCCGTTGGCGTGGACGTTGCCGCCGTAGCGGAATGTGCTGGGGGTGGGG13.0303030303030303No Hit
GTTGTCTTCAGCTGAGTGCTTATTTTCTTCCTTCAGTTTCTCTGGGGTTT13.0303030303030303No Hit
GCTTTTTAATTTACTCGTGCCCTGCAGGTCCTCTCAGAACTAATGGAAAC13.0303030303030303No Hit
CTATTTAGTTACATTCAGCTAGTGATTAATGACTTCATTCCCCTCCTACA13.0303030303030303No Hit
CAAATAAGCAACCTAACTTGATACCTCAAGGAAACAGAAAAGAACAAATA13.0303030303030303No Hit
GCTCCAGCTGGAGCAGGGAATGGTTTCCAGCCTGACTTGGCTGCCTGAGC13.0303030303030303No Hit
CATTTGAAGAAAAGACGATCAAGATTCCACAATATCAAGTGCAATATCCC13.0303030303030303No Hit
GCGAAAGCAGGGATACCCAGGAGGAGGTCAGGAGATGGGCAGGAGGACTG13.0303030303030303No Hit
GGTCCGGGATGATCATGGCCTGGTAGGTGTTAATCCAATGCCATGCCGAG13.0303030303030303No Hit
GAATGAGACCCTGCCCCAGGGGGAGCCGTGCAGATGAAACATACTGATCT13.0303030303030303No Hit
GTTGGAATAATAGTTGATCAGTATCAGAGGGATTCCCTACATCTATGCAC13.0303030303030303No Hit
CAGACAGCCAGGTGGGGTGGGTTTACTTGGCTTGCAGTAGGTTACCTGAG13.0303030303030303No Hit
CAGTTACTCAACTGGTGCGCTTGCCAGTTGCATGGGTCTCATATACAACC13.0303030303030303No Hit
GGTAAAGGTCATGGACAGAAGAAAGGCAATTTTACACTTCACAAGTAACT13.0303030303030303No Hit
GGTAGGGGGTCCTTATCTGCCAGTGGTAGAGCTGGTGACTGCTGGCATCT13.0303030303030303No Hit
TGGTTATCCAGTGGCTTGTCTGCAAGGCTCAGGGAGAACATTCACATCCT13.0303030303030303No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)