FastQCFastQC Report
Sat 14 May 2022
HT5MLBGXL_n02_New_FCC_PCR_Neg_rep2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHT5MLBGXL_n02_New_FCC_PCR_Neg_rep2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences166535
Sequences flagged as poor quality0
Sequence length151
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TATTAAAACTGGGAGTGACAGAAGAAAGAAAAGCAAGAATAGAGACCCTA51583.0972468249917435No Hit
CATTATAAGGACCCCCAGGAAATCTCCCGTCCAGTATCAGCACCCAGAAA42572.556219413336536No Hit
CATAAAATAGAACATCATAGCATATTAAAACTGGGAGTGACAGAAGAAAG35322.1208754916383943No Hit
AAATAGAACATCATAGCATATTAAAACTGGGAGTGACAGAAGAAAGAAAA32861.9731587954484042No Hit
CCCACATAGTGTCCTCATTATAAGGACCCCCAGGAAATCTCCCGTCCAGT29001.741375686792566No Hit
TCATAGCATATTAAAACTGGGAGTGACAGAAGAAAGAAAAGCAAGAATAG27421.646500735581109No Hit
GGTGAGGAAGGCCCCCACATAGTGTCCTCATTATAAGGACCCCCAGGAAA26121.5684390668628214No Hit
CCCCCACATAGTGTCCTCATTATAAGGACCCCCAGGAAATCTCCCGTCCA22231.3348545350827155No Hit
ACATAAAATAGAACATCATAGCATATTAAAACTGGGAGTGACAGAAGAAA20551.2339748401236976No Hit
CATCATAGCATATTAAAACTGGGAGTGACAGAAGAAAGAAAAGCAAGAAT16550.9937850902212749No Hit
AAGCAGGACACATAAAATAGAACATCATAGCATATTAAAACTGGGAGTGA15770.9469480889903024No Hit
CACATAGTGTCCTCATTATAAGGACCCCCAGGAAATCTCCCGTCCAGTAT15750.9457471402407902No Hit
AGCGAAAGCAGGACACATAAAATAGAACATCATAGCATATTAAAACTGGG12580.75539676344312No Hit
TTATAAGGACCCCCAGGAAATCTCCCGTCCAGTATCAGCACCCAGAAAGG12030.7223706728315369No Hit
AGCGAAAGCAGGTGAGGAAGGCCCCCACATAGTGTCCTCATTATAAGGAC9270.5566397453988651No Hit
GTATCAGCACCCAGAAAGGAAGACCTCCCCAGGAGCTAACTGGGGACTTT9190.5518359504008167No Hit
ATAGAACATCATAGCATATTAAAACTGGGAGTGACAGAAGAAAGAAAAGC8410.5049989491698442No Hit
ACCCTAAGCAGAGGCAGGAATGAGGCTAAAATTGCAGACCAGAACGACCC8390.5037980004203321No Hit
CATAGTGTCCTCATTATAAGGACCCCCAGGAAATCTCCCGTCCAGTATCA8250.4953913591737472No Hit
ATAGTGTCCTCATTATAAGGACCCCCAGGAAATCTCCCGTCCAGTATCAG8090.48578376917765037No Hit
GTCCAGTATCAGCACCCAGAAAGGAAGACCTCCCCAGGAGCTAACTGGGG7960.47797760230582165No Hit
GGCCCCCACATAGTGTCCTCATTATAAGGACCCCCAGGAAATCTCCCGTC7790.4677695379349686No Hit
ATTATAAGGACCCCCAGGAAATCTCCCGTCCAGTATCAGCACCCAGAAAG7780.4671690635602126No Hit
CCCCAGGAGCTAACTGGGGACTTTTCACAATATGGACAGAAGGTGCTGGT7740.4647671660611884No Hit
CCCTAAGCAGAGGCAGGAATGAGGCTAAAATTGCAGACCAGAACGACCCT7510.45095625544179907No Hit
GCCCCCACATAGTGTCCTCATTATAAGGACCCCCAGGAAATCTCCCGTCC7240.4347434473233855No Hit
GAACATCATAGCATATTAAAACTGGGAGTGACAGAAGAAAGAAAAGCAAG6980.41913111357972804No Hit
ACATCATAGCATATTAAAACTGGGAGTGACAGAAGAAAGAAAAGCAAGAA6860.41192542108265534No Hit
GTCCTCATTATAAGGACCCCCAGGAAATCTCCCGTCCAGTATCAGCACCC6490.38970786921668116No Hit
GTGTCCTCATTATAAGGACCCCCAGGAAATCTCCCGTCCAGTATCAGCAC6460.38790644609241304No Hit
CAGGTGAGGAAGGCCCCCACATAGTGTCCTCATTATAAGGACCCCCAGGA6120.36749031735070703No Hit
ACATAGTGTCCTCATTATAAGGACCCCCAGGAAATCTCCCGTCCAGTATC5800.34827513735851323No Hit
GAATAGAGACCCTAAGCAGAGGCAGGAATGAGGCTAAAATTGCAGACCAG5560.3338637523643678No Hit
CTCCTTGCCATTGCCAACATTCTTTGCCCCACTTGGAAAAGTCTCTCTCA5440.3266580598672952No Hit
GAAGAAAGAAAAGCAAGAATAGAGACCCTAAGCAGAGGCAGGAATGAGGC5350.32125379049449065No Hit
CCCCCAGGAAATCTCCCGTCCAGTATCAGCACCCAGAAAGGAAGACCTCC5220.31344762362266193No Hit
GAAAGCAGGTGAGGAAGGCCCCCACATAGTGTCCTCATTATAAGGACCCC5220.31344762362266193No Hit
CCTCATTATAAGGACCCCCAGGAAATCTCCCGTCCAGTATCAGCACCCAG5190.31164620049839376No Hit
AACTGGGAGTGACAGAAGAAAGAAAAGCAAGAATAGAGACCCTAAGCAGA5190.31164620049839376No Hit
GGAAATCTCCCGTCCAGTATCAGCACCCAGAAAGGAAGACCTCCCCAGGA5170.3104452517488816No Hit
ATATTAAAACTGGGAGTGACAGAAGAAAGAAAAGCAAGAATAGAGACCCT5060.303840033626565No Hit
GAGATGGAGATGGCCATGAGAATGCAAGTTGTGCATTTGAAAGTCACAAA4940.2966343411294923No Hit
GGACACATAAAATAGAACATCATAGCATATTAAAACTGGGAGTGACAGAA4840.2906295973819317No Hit
CTTCCACTCTCACCCCTTACCTCCTTTTGTCCCCTTACTTTCACACTCCT4830.29002912300717565No Hit
AAATAATACAGAAGTGTTTAGCATAAAGAGTACTAGTCCCTGGCCCTGCT4700.2822229561353469No Hit
CAAGAATAGAGACCCTAAGCAGAGGCAGGAATGAGGCTAAAATTGCAGAC4460.2678115711412016No Hit
ATAAGGACCCCCAGGAAATCTCCCGTCCAGTATCAGCACCCAGAAAGGAA4450.2672110967664455No Hit
CTCTCACCCCTTACCTCCTTTTGTCCCCTTACTTTCACACTCCTTGCCAT4450.2672110967664455No Hit
GTACTAGTCCCTGGCCCTGCTCTCACTAACTGGAAGAAATTGCCCTTGTA4360.261806827393641No Hit
GCATAAAGAGTACTAGTCCCTGGCCCTGCTCTCACTAACTGGAAGAAATT4350.26120635301888495No Hit
GGCAGAGAGAGAGGAGGAAGCAGGCTCCCCGCTGAGCAGAGAGCCCGATG4340.2606058786441289No Hit
GAAAGGAAGACCTCCCCAGGAGCTAACTGGGGACTTTTCACAATATGGAC4330.2600054042693728No Hit
AAAGCAGGCAGAGAGGCAGGCAGAGAGAGAGGAGGAAGCAGGCTCCCCGC4320.2594049298946167No Hit
GAAATCTCCCGTCCAGTATCAGCACCCAGAAAGGAAGACCTCCCCAGGAG4230.2540006605218122No Hit
GGCCATGAGAATGCAAGTTGTGCATTTGAAAGTCACAAAGGTCCACAAAG4180.2509982886480319No Hit
GAGGAAGGCCCCCACATAGTGTCCTCATTATAAGGACCCCCAGGAAATCT4170.2503978142732759No Hit
CACATAAAATAGAACATCATAGCATATTAAAACTGGGAGTGACAGAAGAA4160.24979733989851982No Hit
GCAGGAAGGAGCTCAGAGATGGAGATGGCCATGAGAATGCAAGTTGTGCA4020.24139069865193502No Hit
CTCCCGTCCAGTATCAGCACCCAGAAAGGAAGACCTCCCCAGGAGCTAAC3830.22998168553156995No Hit
ATAGAGACCCTAAGCAGAGGCAGGAATGAGGCTAAAATTGCAGACCAGAA3790.2275797880325457No Hit
ATACTAAGGAGGTCACTTTCTGTGATGAGCACTGGGTATTACATGTTAGT3730.22397694178400937No Hit
AAGCAGGTGAGGAAGGCCCCCACATAGTGTCCTCATTATAAGGACCCCCA3620.21737172366169275No Hit
CAATAAACTTAGCGCTGGTGGAGGGGTGGTGGGTGGAGGGATGAGCTAAA3470.20836460804035187No Hit
CAATATGGACAGAAGGTGCTGGTAGCTGACCAGGCTTCCCTTTTCCCCAG3470.20836460804035187No Hit
ATATATATCATGCTAACACAAATCATAAGAAAACTGAGTGTCTGGGGTGC3400.20416128741705947No Hit
CCCCTTACCTCCTTTTGTCCCCTTACTTTCACACTCCTTGCCATTGCCAA3360.20175938991803524No Hit
ATTAAAACTGGGAGTGACAGAAGAAAGAAAAGCAAGAATAGAGACCCTAA3360.20175938991803524No Hit
CCCGTAAGAGACTCTAACTACAGACAATAAACTTAGCGCTGGTGGAGGGG3300.1981565436694989No Hit
CCACATAGTGTCCTCATTATAAGGACCCCCAGGAAATCTCCCGTCCAGTA3260.19575464617047467No Hit
GCAAGAATAGAGACCCTAAGCAGAGGCAGGAATGAGGCTAAAATTGCAGA3240.19455369742096257No Hit
GTATTATTTTGTGATTTATTTATTAATTGTTAAACAATTATGGAAATACA3210.1927522742966944No Hit
TTCACACTCCTTGCCATTGCCAACATTCTTTGCCCCACTTGGAAAAGTCT3190.19155132554718227No Hit
TTAAAACTGGGAGTGACAGAAGAAAGAAAAGCAAGAATAGAGACCCTAAG3190.19155132554718227No Hit
GGGAGTGACAGAAGAAAGAAAAGCAAGAATAGAGACCCTAAGCAGAGGCA3130.18794847929864594No Hit
GGCAGAGAGGCAGGCAGAGAGAGAGGAGGAAGCAGGCTCCCCGCTGAGCA3120.1873480049238899No Hit
ATACAGAAGTGTTTAGCATAAAGAGTACTAGTCCCTGGCCCTGCTCTCAC3110.18674753054913382No Hit
AAGCAGAGGCAGGAATGAGGCTAAAATTGCAGACCAGAACGACCCTTGTA3100.18614705617437777No Hit
CCCAGAAAGGAAGACCTCCCCAGGAGCTAACTGGGGACTTTTCACAATAT3070.1843456330501096No Hit
ATGTAGATGAAGACACAACATTCATTTTGGAAGACAGGAAGATATAGAAC3050.18314468430059747No Hit
CCCTATATGTTAATAAATAGAATTTAAATAAAAGTGTGAAAAAAAGAAAA3040.18254420992584142No Hit
CCTTTTGTCCCCTTACTTTCACACTCCTTGCCATTGCCAACATTCTTTGC3030.18194373555108534No Hit
CTTTCACACTCCTTGCCATTGCCAACATTCTTTGCCCCACTTGGAAAAGT2980.17894136367730507No Hit
GCATATTAAAACTGGGAGTGACAGAAGAAAGAAAAGCAAGAATAGAGACC2980.17894136367730507No Hit
ATATAGAACAGATAACAGGGACACAGAGAGGGAGGGATAGCTCGTGGGAG2940.17653946617828084No Hit
GGCAGGAATGAGGCTAAAATTGCAGACCAGAACGACCCTTGTATACGCTT2920.17533851742876871No Hit
GGACAGAAGGTGCTGGTAGCTGACCAGGCTTCCCTTTTCCCCAGTGGCTG2890.17353709430450057No Hit
AAAGCAGACCCAGTGAGAAACAGCAAAAATAATTTGAAAACGTTTTTCTC2880.17293661992974452No Hit
GATTTGTCCTGCTGGCCATGTTTTACTGACCCATCCTCTAGAAGAACTAT2880.17293661992974452No Hit
CATATAGGGTATTATTGGTTTCCGGAGTAGAATTTAGTGATTCATCACTA2850.17113519680547634No Hit
AGAATAGAGACCCTAAGCAGAGGCAGGAATGAGGCTAAAATTGCAGACCA2830.16993424805596422No Hit
GACCCTAAGCAGAGGCAGGAATGAGGCTAAAATTGCAGACCAGAACGACC2830.16993424805596422No Hit
ATAATACAGAAGTGTTTAGCATAAAGAGTACTAGTCCCTGGCCCTGCTCT2810.1687332993064521No Hit
GCTCTAGGGGGGTCCCAAGCCAGCAGCCACTGGGGAAAAGGGAAGCCTGG2810.1687332993064521No Hit
CTCTAGAAGAACTATAAACATTAAAAAAAAGTTTTACAGCCCTCTTCTCA2790.16753235055693996No Hit
GCGAAAGCAGGACACATAAAATAGAACATCATAGCATATTAAAACTGGGA2720.1633290299336476No Hit
AAAGCAGGCTTCCCCTTCCACTCTCACCCCTTACCTCCTTTTGTCCCCTT2690.16152760680937941No Hit
GTATAAGACTTTGGGCCAGTCATTTAAGCGCTCTTGGCCTTTGAGGTAAT2690.16152760680937941No Hit
GCGAAAGCAGGTGAGGAAGGCCCCCACATAGTGTCCTCATTATAAGGACC2670.1603266580598673No Hit
CTCTAACTACAGACAATAAACTTAGCGCTGGTGGAGGGGTGGTGGGTGGA2660.15972618368511124No Hit
CTCAAGCACTCTGCTTATTCAAATCCTGCCTGTGCTTTAGGGCACAACCT2660.15972618368511124No Hit
CTTTTAGCTCTAGGGGGGTCCCAAGCCAGCAGCCACTGGGGAAAAGGGAA2640.15852523493559914No Hit
TCCCAGGACCCTGAGATCATGATCTGAGCCGAAGGCAGCGGCTTAACCCA2620.15732428618608701No Hit
GGAGCTAACTGGGGACTTTTCACAATATGGACAGAAGGTGCTGGTAGCTG2590.15552286306181884No Hit
CCCCACATAGTGTCCTCATTATAAGGACCCCCAGGAAATCTCCCGTCCAG2510.1507190680637704No Hit
GTATACGCTTTTAGCTCTAGGGGGGTCCCAAGCCAGCAGCCACTGGGGAA2480.14891764493950221No Hit
GTTTAGCATAAAGAGTACTAGTCCCTGGCCCTGCTCTCACTAACTGGAAG2470.14831717056474614No Hit
GGAAGGCCCCCACATAGTGTCCTCATTATAAGGACCCCCAGGAAATCTCC2460.1477166961899901No Hit
GCTCAGAGATGGAGATGGCCATGAGAATGCAAGTTGTGCATTTGAAAGTC2440.14651574744047796No Hit
GACCAGATTTGTCCTGCTGGCCATGTTTTACTGACCCATCCTCTAGAAGA2420.14531479869096586No Hit
GCAGGACACATAAAATAGAACATCATAGCATATTAAAACTGGGAGTGACA2400.14411384994145376No Hit
TTACTGACCCATCCTCTAGAAGAACTATAAACATTAAAAAAAAGTTTTAC2390.1435133755666977No Hit
GTATTACATGTTAGTGATGAATCACTAAATTCTACTCCGGAAACCAATAA2380.14291290119194164No Hit
GATTTATTATGAGCATATATTTCTTTTGTAATTAAAAAAGGAATGAAAGT2350.14111147806767346No Hit
TGACAGAAGAAAGAAAAGCAAGAATAGAGACCCTAAGCAGAGGCAGGAAT2310.13870958056864924No Hit
GTGGAGGGGTGGTGGGTGGAGGGATGAGCTAAATGGGTGATGGATACTAA2300.13810910619389316No Hit
ATTATGAGCATATATTTCTTTTGTAATTAAAAAAGGAATGAAAGTTTGGA2290.1375086318191371No Hit
TATCAGCACCCAGAAAGGAAGACCTCCCCAGGAGCTAACTGGGGACTTTT2290.1375086318191371No Hit
ACCTCCTTTTGTCCCCTTACTTTCACACTCCTTGCCATTGCCAACATTCT2270.136307683069625No Hit
AAGGAAGACCTCCCCAGGAGCTAACTGGGGACTTTTCACAATATGGACAG2220.1333053111958447No Hit
ATATTAAATAAATTACTTATGATTTATTATGAGCATATATTTCTTTTGTA2220.1333053111958447No Hit
GGCTAAAATTGCAGACCAGAACGACCCTTGTATACGCTTTTAGCTCTAGG2170.13030293932206444No Hit
AAAGCAGGCAGCAGACCAGATTTGTCCTGCTGGCCATGTTTTACTGACCC2160.12970246494730836No Hit
CTCCCACCCCCATGTCCCACAGAAGTTTGTGTGTGTGTTTCTTTTCCAAA2150.12910199057255234No Hit
AAAGAGTACTAGTCCCTGGCCCTGCTCTCACTAACTGGAAGAAATTGCCC2140.12850151619779626No Hit
GCCCTAAAGAGATAGCGAGATCTCAACTGATACCTGCAAACACTTCCCCA2110.1267000930735281No Hit
TGTATACGCTTTTAGCTCTAGGGGGGTCCCAAGCCAGCAGCCACTGGGGA2110.1267000930735281No Hit
AGCGAAAGCAGGCAGAGAGGCAGGCAGAGAGAGAGGAGGAAGCAGGCTCC2100.12609961869877204No Hit
AGCAAGAATAGAGACCCTAAGCAGAGGCAGGAATGAGGCTAAAATTGCAG2090.12549914432401596No Hit
ACTTATGATTTATTATGAGCATATATTTCTTTTGTAATTAAAAAAGGAAT2070.12429819557450386No Hit
GATGAAGACACAACATTCATTTTGGAAGACAGGAAGATATAGAACAGATA2070.12429819557450386No Hit
GAGTGACAGAAGAAAGAAAAGCAAGAATAGAGACCCTAAGCAGAGGCAGG2040.1224967724502357No Hit
ACCCGTAAGAGACTCTAACTACAGACAATAAACTTAGCGCTGGTGGAGGG2040.1224967724502357No Hit
GCAGGTGAGGAAGGCCCCCACATAGTGTCCTCATTATAAGGACCCCCAGG2030.12189629807547962No Hit
TGTTTATTTAATATTAAATAAATTACTTATGATTTATTATGAGCATATAT1980.11889392620169933No Hit
GAATGCAAGTTGTGCATTTGAAAGTCACAAAGGTCCACAAAGTTCTGTGG1980.11889392620169933No Hit
TGCTAACACAAATCATAAGAAAACTGAGTGTCTGGGGTGCCTGGGTGGCT1970.11829345182694329No Hit
GGGTATTACATGTTAGTGATGAATCACTAAATTCTACTCCGGAAACCAAT1960.11769297745218724No Hit
CCCTTCCACTCTCACCCCTTACCTCCTTTTGTCCCCTTACTTTCACACTC1940.11649202870267511No Hit
CCCAGGAAATCTCCCGTCCAGTATCAGCACCCAGAAAGGAAGACCTCCCC1930.11589155432791905No Hit
AGCGAAAGCAGGGAAGGAAGGAAGGAAATCGAAAGCTAACGATGTAGATG1930.11589155432791905No Hit
GACCAGAACGACCCTTGTATACGCTTTTAGCTCTAGGGGGGTCCCAAGCC1920.115291079953163No Hit
ATAAAAGGGTTTGTCCCACAAGTAGGAAGATAATGCAATAATAGCTGAGA1910.11469060557840695No Hit
GTGAGAAACAGCAAAAATAATTTGAAAACGTTTTTCTCTCACTAAGCTGT1880.11288918245413877No Hit
CTCTCAAGCACTCTGCTTATTCAAATCCTGCCTGTGCTTTAGGGCACAAC1880.11288918245413877No Hit
GACAGAAGGTGCTGGTAGCTGACCAGGCTTCCCTTTTCCCCAGTGGCTGC1870.11228870807938271No Hit
GAAATAAATAGTCCAAGTTTATCTTAATAGGCCCTAAAGAGATAGCGAGA1860.11168823370462666No Hit
ACTTAGCGCTGGTGGAGGGGTGGTGGGTGGAGGGATGAGCTAAATGGGTG1850.11108775932987061No Hit
GCAGAGAGGCAGGCAGAGAGAGAGGAGGAAGCAGGCTCCCCGCTGAGCAG1810.10868586183084637No Hit
ATCATGATCTGAGCCGAAGGCAGCGGCTTAACCCACTGAGCCACCCAGGC1810.10868586183084637No Hit
CAGTATCAGCACCCAGAAAGGAAGACCTCCCCAGGAGCTAACTGGGGACT1810.10868586183084637No Hit
TTTCACAATATGGACAGAAGGTGCTGGTAGCTGACCAGGCTTCCCTTTTC1780.1068844387065782No Hit
GTCAAACAACCATTCAGAACAGCAGTTCTCCGTTTCTACCTTTCATTTTC1770.10628396433182215No Hit
GAGGCAGGCAGAGAGAGAGGAGGAAGCAGGCTCCCCGCTGAGCAGAGAGC1770.10628396433182215No Hit
GTCCCTGGCCCTGCTCTCACTAACTGGAAGAAATTGCCCTTGTAGAATTC1750.10508301558231004No Hit
GGCTTCCCCTTCCACTCTCACCCCTTACCTCCTTTTGTCCCCTTACTTTC1740.10448254120755396No Hit
ACTGGGGACTTTTCACAATATGGACAGAAGGTGCTGGTAGCTGACCAGGC1730.10388206683279791No Hit
AATAGAGACCCTAAGCAGAGGCAGGAATGAGGCTAAAATTGCAGACCAGA1690.10148016933377368No Hit
CAATTATGGAAATACACAAATGTTTATTTAATATTAAATAAATTACTTAT1680.10087969495901762No Hit
CCCTTGTATACGCTTTTAGCTCTAGGGGGGTCCCAAGCCAGCAGCCACTG1680.10087969495901762No Hit
GAGTAGAAGGCAGTAAATGGCTAGCTCAGTGAGTGTCCCAATTGGATTTA1670.10027922058426157No Hit
GGGAAGGAAGGAAGGAAATCGAAAGCTAACGATGTAGATGAAGACACAAC1670.10027922058426157No Hit
TAGAGACCCTAAGCAGAGGCAGGAATGAGGCTAAAATTGCAGACCAGAAC1670.10027922058426157No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)