FastQCFastQC Report
Sat 14 May 2022
HT5MLBGXL_n01_New_Sp19_2232_d08_rep2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHT5MLBGXL_n01_New_Sp19_2232_d08_rep2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2519193
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTCCAACACTGTGTCAAGCTTTCAGGTAGACTGCTTTCTTTGGCATGTCC182940.7261849330321257No Hit
AGCAGGTACTGATCCAAAATGGAAGACTTTGTGCGACAGTGCTTCAATCC176470.7005021052376693No Hit
TTTTTGGACAGTATGGATAGCAAATAGTAGCATTGCCACAACTATTTTAG165260.6560037281780317No Hit
GTATGGATAGCAAATAGTAGCATTGCCACAACTATTTTAGTGCATGTGTG163350.648421935119699No Hit
GAGCAAAAGCAGGTACTGATCCAAAATGGAAGACTTTGTGCGACAGTGCT146430.5812575693882922No Hit
GAGCAAAAGCAGGGTGACAAAAACATAATGGATTCCAACACTGTGTCAAG105620.41926124755030675No Hit
GTTGTAAGGCTTGCATAAAGGTTATTTGCTCAAAGCTATTCTCTGTAATT99530.395086839317194No Hit
GAGCAAAAGCAGGCAAACCATTTGAATGGATGTCAATCCGACTTTACTTT80890.3210948903081265No Hit
CACTTAGAAGTCTGCTTCATGTATTCAGACTTCCATTTCATTGACGAACG78440.3113695536626213No Hit
GAGCAAAAGCAGGTCAAATATATTCAATATGGAAAGAATAAAAGAACTAA69990.2778270660485322No Hit
ATTTTAGTGCATGTGTGAGGAAGGAGTTGAACCAAGATGCATTAAGCAAA68950.2736987598806443No Hit
CCCTTATACTGGAGATCCCCCATATAGCCATGGAACAGGAACAGGATACA67240.2669108718545979No Hit
TTCTTAACCTATGTCGCATTTCTTCAATTAACCATCTTATCTCTTCAAAC66500.26397342323513917No Hit
GTGTTATCATTCCATTCAAGTCCTCCGATGAGGACCCCAATTGCATTTTT62820.24936557064107434No Hit
AAACAAGGGTGTTTTTTATCATTAAATAAGCTGAAACGAGAAAGTTCTTA57940.2299942878532927No Hit
TTTTTTGGACAGTATGGATAGCAAATAGTAGCATTGCCACAACTATTTTA56250.22328579033047488No Hit
AAGCTATTCTCTGTAATTCTTAACCTATGTCGCATTTCTTCAATTAACCA55780.22142011350460244No Hit
GCAAAAGCAGGGTGACAAAAACATAATGGATTCCAACACTGTGTCAAGCT55750.22130102774975952No Hit
GCAAAAGCAGGTACTGATCCAAAATGGAAGACTTTGTGCGACAGTGCTTC52540.20855885198156712No Hit
GCATAAAGGTTATTTGCTCAAAGCTATTCTCTGTAATTCTTAACCTATGT52160.20705043242022345No Hit
GAATAAAAGAACTAAGAAATTTGATGTCGCAATCTCGCACTCGCGAGATA51420.20411298380076476No Hit
AGTAGAAACAAGGCATTTTTTCATGAAGGACAAGCCAAATTCACTTCCCT50820.20173126870390634No Hit
GCATATAGTGGAGCGGATTCTGGAGGAAGAATCTGACGAGGCACTTAAAA50270.1995480298651195No Hit
ACCTAACTGAAATGACTCTTGAGGAAATGTCAAGGGATTGGTTAATGCTC48270.19160897954225817No Hit
GTTCTTATCTCTTGCTCCACTTCAAGCAATAGTTGTAAGGCTTGCATAAA47390.1881157974001992No Hit
CTATTGCTCCTTCATCTGTAAACGCTCTAAGTAGTATCAGAGCTTCAAGT46510.1846226152581402No Hit
GTAATGGGTAAAACCATCATATTGAAAGCAAACTTTAGTGTGATTTTTAA45770.1816851666386815No Hit
GTGTCAAGCTTTCAGGTAGACTGCTTTCTTTGGCATGTCCGCAAACGATT45100.17902558478052297No Hit
GGTTAATGCTCATTCCCAAGCAGAAAGTGACAGGGCCCCTTTGCATTAGA44740.17759655572240793No Hit
AGAATGGACCAGGCAGTAATGGGTAAAACCATCATATTGAAAGCAAACTT44630.17715990795465056No Hit
TTTTTAAACAATTCGACACTAATTGATGGCCATCCGAATTCTTTTGGTCG43080.17100714395443303No Hit
CTGTAATTCTTAACCTATGTCGCATTTCTTCAATTAACCATCTTATCTCT42460.168546038354346No Hit
GTAGAAACAAGGGTGTTTTTTATCATTAAATAAGCTGAAACGAGAAAGTT41610.16517194196712995No Hit
ATGGAAGACTTTGTGCGACAGTGCTTCAATCCAATGATTGTCGAGCTTGC41530.1648543799542155No Hit
TAATTAAGAAGTACACATCAGGAAGACAGGAGAAGAATCCCGCTCTTAGA41180.16346504614771476No Hit
ATATAGCCCTTCAAGATCAAAGGTTCCAGGTTCCAGGTTGTCCCTAAGTG40160.15941613048305547No Hit
CTATTTTAGTGCATGTGTGAGGAAGGAGTTGAACCAAGATGCATTAAGCA38200.1516358611666514No Hit
CTCCTCAATTGCTCCATATAGCCCTTCAAGATCAAAGGTTCCAGGTTCCA38170.15151677541180847No Hit
CTCTTGGTCTGGACATCAGAACTGCCACTCGTGAAGGAAAGCATATAGTG35820.14218839128244642No Hit
CTCTTGCTCCACTTCAAGCAATAGTTGTAAGGCTTGCATAAAGGTTATTT35600.14131509574693166No Hit
GTCCTGGAAGGGAAGGTAATGGTGAGATTTCGCCCACTATTGCTCCTTCA34150.1355592842628572No Hit
AATATATTCAATATGGAAAGAATAAAAGAACTAAGAAATTTGATGTCGCA34110.13540050325639996No Hit
GCAAATAGTAGCATTGCCACAACTATTTTAGTGCATGTGTGAGGAAGGAG33660.13361421693375616No Hit
GAGTGGAGATCTCCCATTCTCATCACTGCTTCTCCAAGTGAATCTCTGTA33480.13289970240469864No Hit
GCAAAAGCAGGTCAAATATATTCAATATGGAAAGAATAAAAGAACTAAGA32910.13063707306268316No Hit
AAGTAGTATCAGAGCTTCAAGTCGATTAAAAATCACACTAAAGTTTGCTT32650.1296049965207112No Hit
ATATAGTGGAGCGGATTCTGGAGGAAGAATCTGACGAGGCACTTAAAATG32370.1284935294755106No Hit
CCCTAAGAGGAAGAGGCAGCACTCTTGGTCTGGACATCAGAACTGCCACT31360.12448430906246565No Hit
ACTTAGAGCGTTTACAGATGAAGGAGCAATAGTGGGCGAAATCTCACCAT30350.12047508864942066No Hit
CTTCAATGGTGGAACAGATCTTCAAGATCTCAGCAAATTCTTCTTTCTTA30210.11991935512682037No Hit
GGGTAAAACCATCATATTGAAAGCAAACTTTAGTGTGATTTTTAATCGAC29990.11904605959130563No Hit
ATCCAATGATTGTCGAGCTTGCGGAAAAGACAATGAAGGAATATGGGGAA29570.11737885902350476No Hit
CTCCCATTCTCATCACTGCTTCTCCAAGTGAATCTCTGTAGAGTTTCAGA29280.11622769672668985No Hit
CAGTATGGATAGCAAATAGTAGCATTGCCACAACTATTTTAGTGCATGTG28840.11448110565566037No Hit
CTTAGAAGTCTGCTTCATGTATTCAGACTTCCATTTCATTGACGAACGAG28360.11257573357817365No Hit
TTGTGCGACAGTGCTTCAATCCAATGATTGTCGAGCTTGCGGAAAAGACA28090.11150396178458737No Hit
GCATTAGAATGGACCAGGCAGTAATGGGTAAAACCATCATATTGAAAGCA28040.11130548552651584No Hit
TAGTAGCATTGCCACAACTATTTTAGTGCATGTGTGAGGAAGGAGTTGAA28030.11126579027490152No Hit
CTTAAAATGACTATCGCTTCAGTGCCTGCTTCACGCTACCTAACTGAAAT27430.10888407517804313No Hit
GGTTGTCCCTAAGTGCTTGAACAATGAGTAGTAGTTTTCTCGATTCAGCT27290.10832834165544283No Hit
GGTCAAATATATTCAATATGGAAAGAATAAAAGAACTAAGAAATTTGATG27110.10761382712638531No Hit
GTTTTTTATCATTAAATAAGCTGAAACGAGAAAGTTCTTATCTCTTGCTC27060.10741535086831377No Hit
GCATTAAGCAAAACCCAGGGATCATTAATCAGGCACTCCTCAATTGCTCC26700.10598632181019875No Hit
TTCCTTGACCGGCTTCGCCGAGATCAGAAGTCCCTAAGAGGAAGAGGCAG26480.10511302627468398No Hit
ACATAATGGATTCCAACACTGTGTCAAGCTTTCAGGTAGACTGCTTTCTT26180.1039221687262548No Hit
AGTATGGATAGCAAATAGTAGCATTGCCACAACTATTTTAGTGCATGTGT25910.10285039693266852No Hit
ATACTGGAGATCCCCCATATAGCCATGGAACAGGAACAGGATACACCATG25830.10253283491975407No Hit
GATCTTCAAGATCTCAGCAAATTCTTCTTTCTTAATCCTTCCAGACTCGA25520.10130228211971057No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)