FastQCFastQC Report
Sat 14 May 2022
HT5MLBGXL_n01_New_P1_SynRNA_10_rep2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHT5MLBGXL_n01_New_P1_SynRNA_10_rep2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2037500
Sequences flagged as poor quality0
Sequence length151
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCTTATACAATCCAGCCCTGTTAGTTCTGGATGCTGGACAAAACTCCCG119360.5858159509202454No Hit
ACTCAGCACCGTCCGGCCAAGACCAACCCACAGTGTCACTGTTTACACCA101330.4973251533742331No Hit
GTAGATGGATGGGCTATATACAGTAAAGACAACAGTGTAAGAATCGGTTC82830.4065276073619632No Hit
TATGTATCCTATCTGATATTCCAGATTCTGGTTGAAAGACACCCACGGTC82570.4052515337423313No Hit
GGATACATATGTAGTGGGATTTTCGGAGACAATCCACGCCCTAATGATAA65140.3197055214723926No Hit
ATGTATAGGTTATCATGCGAACAATTCAACAGACACTGTAGACACAGTAC58740.28829447852760737No Hit
GAGCAAAAGCAGGTCAATTATATTCAATATGGAGAGAATAAAAGAACTGA58520.28721472392638037No Hit
ATTCTACACTGTAGAGACCAATTAGAGCACATCCAGAAACTGATTGCCCC57770.2835337423312883No Hit
CTACATATGTATCCTATCTGATATTCCAGATTCTGGTTGAAAGACACCCA57570.2825521472392638No Hit
TTTTTAAACTATTCGACAATTAATTGATGGCCGCCCGAATTCTTTTGGTC56800.2787730061349693No Hit
CTGTAGACACAGTACTAGAAAAGAATGTAACAGTAACACACTCTGTTAAC56200.27582822085889575No Hit
CATATGTAGTGGGATTTTCGGAGACAATCCACGCCCTAATGATAAGACAG55770.27371779141104297No Hit
GATGTATATTATGAAATGGGAGGCTGGTGTTTATAGCACCCTTGGGTGTC52840.2593374233128834No Hit
CTATCAAGAGTTGGAGAAACAATATATTGAGAACACAAGAGTCTGAATGT50100.24588957055214725No Hit
GCTATATACAGTAAAGACAACAGTGTAAGAATCGGTTCCAAGGGGGATGT48800.2395092024539877No Hit
GTGATGGACAGGCCTCATACAAGATCTTCAGAATAGAAAAGGGAAAGATA48150.23631901840490796No Hit
ATATACAGTAAAGACAACAGTGTAAGAATCGGTTCCAAGGGGGATGTGTT47920.23519018404907976No Hit
GTCCATCACTTGGTCCATCGGTCATTACAGTAAAGCAAGAACCATTTACA47310.23219631901840487No Hit
ATACTGGACCACAACTGCCTGTCTTATCATTAGGGCGTGGATTGTCTCCG46540.22841717791411043No Hit
GTCCCAGTCCATCCGTTCGGATCCCAAATCATCTCAAAACCGTTCCTTGA44800.21987730061349695No Hit
GTCCTACATTGTGGAAACACCTAGTTCAGACAATGGAACGTGTTACCCAG44420.2180122699386503No Hit
GAGCAAAAGCAGGGGAAAATAAAAACAACCAAAATGGAGGCAATACTAGT43290.21246625766871166No Hit
GTATGAGGCCTGTCCATCACTTGGTCCATCGGTCATTACAGTAAAGCAAG43250.21226993865030674No Hit
GGCTATATACAGTAAAGACAACAGTGTAAGAATCGGTTCCAAGGGGGATG43010.2110920245398773No Hit
CAATATGGATTAGCCACTCAATTCAACTTGGGAATCAAAATCAGATTGAA42730.20971779141104294No Hit
GTGTAAGAATCGGTTCCAAGGGGGATGTGTTTGTCATAAGGGAACCATTC40970.201079754601227No Hit
ATCCTGACCATTCATTTATTCCTACGATATCTTGCTTTATTGAGAAGTTA40120.19690797546012268No Hit
GTCCTATTGGTGAAGTGCCCTCTCCATACAACTCAAGATTTGAGTCAGTC40010.19636809815950917No Hit
GCTATATCCTGACCATTCATTTATTCCTACGATATCTTGCTTTATTGAGA39200.1923926380368098No Hit
GTTATTGTCTGTCCCAGTCCATCCGTTCGGATCCCAAATCATCTCAAAAC38990.19136196319018403No Hit
AAAGACAACAGTGTAAGAATCGGTTCCAAGGGGGATGTGTTTGTCATAAG38720.19003680981595092No Hit
TCTCCATACAACTCAAGATTTGAGTCAGTCGCTTGGTCAGCAAGTGCTTG37910.18606134969325155No Hit
GGATTAGCCACTCAATTCAACTTGGGAATCAAAATCAGATTGAAACATGC36430.17879754601226994No Hit
AATTATCACTATGAGGAATGCTCCTGTTATCCTGATTCTAGTGAAATCAC36130.17732515337423313No Hit
CTCCATACAACTCAAGATTTGAGTCAGTCGCTTGGTCAGCAAGTGCTTGT35810.17575460122699385No Hit
GCTATATACATTTGCAACCGCAAATGCAGACACATTATGTATAGGTTATC34900.1712883435582822No Hit
GAATAATAACAGACACTATCAAGAGTTGGAGAAACAATATATTGAGAACA34830.17094478527607362No Hit
GATTACTTGTCAATGGTAAATGGCAACTCAGCACCGTCCGGCCAAGACCA34460.16912883435582823No Hit
ATTAAAGACAGGAGCCCATATCGAACCCTAATGAGCTGTCCTATTGGTGA33930.1665276073619632No Hit
GTGTATGCAGGGATAACTGGCATGGCTCGAATCGACCGTGGGTGTCTTTC33870.16623312883435581No Hit
GTAGAGACCAATTAGAGCACATCCAGAAACTGATTGCCCCCAGGGAGACT33660.16520245398773006No Hit
ACACTGTAGAGACCAATTAGAGCACATCCAGAAACTGATTGCCCCCAGGG33650.16515337423312884No Hit
GTCCAGTATCGTCTAATGGAGCAAATGGAGTAAAAGGATTTTCATTCAAA32950.16171779141104295No Hit
ATCCAAACACCATTGCCGTATTTGAATGAAAATCCTTTTACTCCATTTGC32950.16171779141104295No Hit
CTAATGAGCTGTCCTATTGGTGAAGTGCCCTCTCCATACAACTCAAGATT32880.16137423312883437No Hit
TCATTAGGGCGTGGATTGTCTCCGAAAATCCCACTACATATGTATCCTAT32220.1581349693251534No Hit
ATCTACTAGTGCTGACCAACAAAGCCTCTATCAGAATGCAGATGCATATG31730.15573006134969325No Hit
GAGCAAAAGCAGGGGTTTAAAATGAATCCAAACCAAAAGATAATAACCAT31660.15538650306748467No Hit
GTACTAGAAAAGAATGTAACAGTAACACACTCTGTTAACCTTCTAGAAGA31150.15288343558282208No Hit
GTGTTAAAGTACAACGGAATAATAACAGACACTATCAAGAGTTGGAGAAA31050.15239263803680983No Hit
GTGATAATTAGGGGCATTCATTTCGACTGATTTGACTATCTTTCCCTTTT30810.15121472392638036No Hit
TTCCAATTGTGATCGGATGTATATTATGAAATGGGAGGCTGGTGTTTATA30560.1499877300613497No Hit
GTTCACAGCAGTAGGTAAAGAGTTCAACCACCTGGAAAAAAGAATAGAGA30540.14988957055214724No Hit
TCTCAAAACCGTTCCTTGAACTAATGCTTTTAGTTCTCCCTATCCAAACA30320.14880981595092024No Hit
GTAATGACCGATGGACCAAGTGATGGACAGGCCTCATACAAGATCTTCAG30310.14876073619631902No Hit
GCATACACATGTGATTTCACTAGAATCAGGATAACAGGAGCATTCCTCAT30130.14787730061349694No Hit
ATTATATTCAATATGGAGAGAATAAAAGAACTGAGAGATCTAATGTCGCA29790.1462085889570552No Hit
GTCTTATCATTAGGGCGTGGATTGTCTCCGAAAATCCCACTACATATGTA29630.14542331288343557No Hit
CCCTAATGATAAGACAGGCAGTTGTGGTCCAGTATCGTCTAATGGAGCAA29370.14414723926380368No Hit
ATACAATCCAGCCCTGTTAGTTCTGGATGCTGGACAAAACTCCCGCTATA28770.14120245398773007No Hit
ACAATATATTGAGAACACAAGAGTCTGAATGTGCATGTGTAAATGGTTCT28660.14066257668711657No Hit
CATTATGTATAGGTTATCATGCGAACAATTCAACAGACACTGTAGACACA28600.14036809815950918No Hit
GAATAAAAGAACTGAGAGATCTAATGTCGCAGTCCCGCACTCGCGAGATA28580.14026993865030674No Hit
CCATTAGACGATACTGGACCACAACTGCCTGTCTTATCATTAGGGCGTGG28370.13923926380368098No Hit
TACTAGTGCTGACCAACAAAGCCTCTATCAGAATGCAGATGCATATGTTT28040.13761963190184048No Hit
GTGTCACTGTTTACACCACAAAAAGATATGCTGCTCCCGCTAGTCCAGAT27830.13658895705521473No Hit
CTCTTGATAGTGTCTGTTATTATTCCGTTGTACTTTAACACAGCCACTGC27320.13408588957055215No Hit
GTATGGAGAGGGCACTTCACCAATAGGACAGCTCATTAGGGTTCGATATG26510.13011042944785278No Hit
CTGTTAACCTTCTAGAAGACAAGCATAACGGGAAACTATGCAAACTAAGA26480.1299631901840491No Hit
TGATAAGACAGGCAGTTGTGGTCCAGTATCGTCTAATGGAGCAAATGGAG25940.12731288343558284No Hit
GCAAAAGCAGGTCAATTATATTCAATATGGAGAGAATAAAAGAACTGAGA25790.12657668711656442No Hit
ATGTTAACATCAGCAACACCAACTTTGCTGCTGGACAGTCAGTGGTTTCC25740.12633128834355828No Hit
TTATTATTCCGTTGTACTTTAACACAGCCACTGCCCCATTGTCTGGGCCA25670.1259877300613497No Hit
AATTAGGGGCATTCATTTCGACTGATTTGACTATCTTTCCCTTTTCTATT25600.1256441717791411No Hit
CCCTTGGAATGCAGAACCTTCTTCTTGACTCAAGGGGCCTTGCTAAATGA25580.12554601226993867No Hit
GTGTAAATGGTTCTTGCTTTACTGTAATGACCGATGGACCAAGTGATGGA25340.1243680981595092No Hit
CATTTATTCCTACGATATCTTGCTTTATTGAGAAGTTATTGTCTGTCCCA25330.12431901840490797No Hit
GTCATAAGGGAACCATTCATATCATGCTCCCCCTTGGAATGCAGAACCTT25320.12426993865030675No Hit
AAGATATGCTGCTCCCGCTAGTCCAGATTGTGTTCTCTTTGGGTCGCCCT25040.1228957055214724No Hit
GTTCCAAGGGGGATGTGTTTGTCATAAGGGAACCATTCATATCATGCTCC24990.12265030674846625No Hit
ATCAAGAGTTGGAGAAACAATATATTGAGAACACAAGAGTCTGAATGTGC24970.1225521472392638No Hit
ATATATTGTTTCTCCAACTCTTGATAGTGTCTGTTATTATTCCGTTGTAC24320.11936196319018405No Hit
ATCCAGCCCTGTTAGTTCTGGATGCTGGACAAAACTCCCGCTATATCCTG24220.11887116564417179No Hit
GTTAAAGTACAACGGAATAATAACAGACACTATCAAGAGTTGGAGAAACA24120.11838036809815951No Hit
GCTGTGAACTGTGTATTCATCTTTTCAATAACAGAATTTACTTTGTTAGT24110.11833128834355829No Hit
TTACTGTAATGACCGATGGACCAAGTGATGGACAGGCCTCATACAAGATC24030.11793865030674848No Hit
TCCTTAGACAGAATCCAACTGAGGAACAAGCCGTAGACATATGCAAGGCA23960.11759509202453988No Hit
TCTCAATATGGATTAGCCACTCAATTCAACTTGGGAATCAAAATCAGATT23960.11759509202453988No Hit
CTGTAGATGGATGGGCTATATACAGTAAAGACAACAGTGTAAGAATCGGT23730.11646625766871166No Hit
ATAACAGACACTATCAAGAGTTGGAGAAACAATATATTGAGAACACAAGA23690.11626993865030676No Hit
GTAGAAACAAGGTCGTTTTTAAACTATTCGACAATTAATTGATGGCCGCC23560.11563190184049081No Hit
TTTTTGAACAGATTACTTGTCAATGGTAAATGGCAACTCAGCACCGTCCG23550.11558282208588956No Hit
GATTAGTTCAACCCAGAAGCAAGGTCTTATACAATCCAGCCCTGTTAGTT23500.11533742331288344No Hit
ACGGCATAATAACAGACACTATCAAGAGTTGGAGAAACAATATATTGAGA23340.11455214723926381No Hit
CCATTTACACATGCACATTCAGACTCTTGTGTTCTCAATATATTGTTTCT23140.11357055214723927No Hit
TGCACATTCAGACTCTTGTGTTCTCAATATATTGTTTCTCCAACTCTTGA23110.11342331288343559No Hit
GTTTACACCACAAAAAGATATGCTGCTCCCGCTAGTCCAGATTGTGTTCT22990.11283435582822086No Hit
GGATAACAGGAGCATTCCTCATAGTGATAATTAGGGGCATTCATTTCGAC22880.11229447852760736No Hit
CCTCAATCCTGTGGCCAGTCTCAATTTTGTGCTTTTTACATATTTTGGAC22760.11170552147239264No Hit
CAGTAAAGACAACAGTGTAAGAATCGGTTCCAAGGGGGATGTGTTTGTCA22710.11146012269938652No Hit
TTACAGTAAAGCAAGAACCATTTACACATGCACATTCAGACTCTTGTGTT22520.1105276073619632No Hit
GAATGTGCATGTGTAAATGGTTCTTGCTTTACTGTAATGACCGATGGACC22290.10939877300613497No Hit
ACATATGTATCCTATCTGATATTCCAGATTCTGGTTGAAAGACACCCACG22160.10876073619631901No Hit
CTATGAGGAATGCTCCTGTTATCCTGATTCTAGTGAAATCACATGTGTAT21980.10787730061349694No Hit
TCCTCATAGTGATAATTAGGGGCATTCATTTCGACTGATTTGACTATCTT21780.1068957055214724No Hit
GTCTAATGGAGCAAATGGAGTAAAAGGATTTTCATTCAAATACGGCAATG21600.1060122699386503No Hit
CAATATATTGAGAACACAAGAGTCTGAATGTGCATGTGTAAATGGTTCTT21080.10346012269938651No Hit
TACATATGTAGTGGGATTTTCGGAGACAATCCACGCCCTAATGATAAGAC21020.10316564417177915No Hit
GTCTGAATGTGCATGTGTAAATGGTTCTTGCTTTACTGTAATGACCGATG20930.1027239263803681No Hit
CTTTAACACAGCCACTGCCCCATTGTCTGGGCCAGAAATTCCAATTGTTA20760.10188957055214723No Hit
CATTCAGACTCTTGTGTTCTCAATATATTGTTTCTCCAACTCTTGATAGT20620.10120245398773006No Hit
GCCCCATTGTCTGGGCCAGAAATTCCAATTGTTAGCCAATTGATGCCATC20510.10066257668711656No Hit
TTCCTGGACATTTGGACTTACAATGCCGAACTGTTGGTTCTATTGGAAAA20460.10041717791411044No Hit
CAGTATAAGAATCGGTTCCAAGGGGGATGTGTTTGTCATAAGGGAACCAT20440.10031901840490798No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)