FastQCFastQC Report
Sat 14 May 2022
HT5MLBGXL_n01_New_FCC_Seq_Neg_rep2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHT5MLBGXL_n01_New_FCC_Seq_Neg_rep2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences33
Sequences flagged as poor quality0
Sequence length151
%GC49

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCCTGGCGGCGGCGCGATGCGCGAAAGCGGGCCGGTGCTGCTAAATTCC26.0606060606060606No Hit
GTTGAGTTCTCAAACATCACACCCACCACAACAATCCCCACACAAACCAT13.0303030303030303No Hit
AAGTAGGCAGAAGGGACAGAGGAGAAGCCCACCAAGCCCAGGGAAAGCAC13.0303030303030303No Hit
GGAATGGAGACCCAAACAACATGGACAGGGCAGTTAAACTATACAAGAAG13.0303030303030303No Hit
ATCCTACATTAATGATAAAGGGAAAGAAGTCCTCGTGCTGTGGGGCATTC13.0303030303030303No Hit
CCGTAATGCTTTATACTGGGGCATTGTATGTTCGAGAAGCAATGTTCTAT13.0303030303030303No Hit
GTTTTTGCCCACTCATGGTTGAGTGGAGGTCAGTCACATGAGGGGAAGAA13.0303030303030303No Hit
GAGATAGAGCAGTCATATCAGTAGCTTGGAATTAAATAAGAATTTACATT13.0303030303030303No Hit
TCTCCTAACTTTGGACTTTATTTGTTCTTTTCTGTTTCCTTGATGTATCA13.0303030303030303No Hit
GCCTGTACAAGGCACTCATGCACAAACAGGAGGCTCCAGCCCTCGAAGCA13.0303030303030303No Hit
TGTAAGCACAAATGAGAAAACTCTAAGAAACATGGATCCAGGAGGATATC13.0303030303030303No Hit
CTGGAAATCTAGTGGTACCGAGATATGCATTCACAATGGAAAGAAATGCT13.0303030303030303No Hit
CTGGACAATATGGTGAAATCCCGTCTCTACTAAAAATACAAAAAGTAGCC13.0303030303030303No Hit
GGATGACCCAAGAACATGACAAAAGTACAGGTTCTTGGGCAAGACCCCAG13.0303030303030303No Hit
TAATATCACCAGTTGGCCAAGTAGTATCCACGCAAGAGTTTCTGAATCTT13.0303030303030303No Hit
TTTTAGACCTGCACTGGAACTAGGGTGAGTCCCAATTGTCCTCATAGCCT13.0303030303030303No Hit
CCCTCCCTCGGGGGACTCCACAGAACAGCGGGCACACTCACAAAACAACT13.0303030303030303No Hit
TACTTCCTTCTGTTGTTTTGTGTACGCTGTGGTGGTGCCGTTTCTGGGAC13.0303030303030303No Hit
GGGGTGTGGGTTGAGATGGGATCAGGTATACAGAAAAGCACACAGAGAGG13.0303030303030303No Hit
GTTGGTCTCAGATGGAGGGCCGAATTTATACAACATCCGGATTCTTCACA13.0303030303030303No Hit
GGGCAGGCTCAGGCCACAGCCAGGGGCCCCTCCACTTCCTTCCCCGGTGT13.0303030303030303No Hit
CTGAAAGTCTCATAACCAGAGCGCGAATCTTTCTTGATTGTTCGTCCCAT13.0303030303030303No Hit
GAGCAAAAGCAGGGGGAGGGGGAGAGGGATAAGTAGGCTTCCAGCTGAAC13.0303030303030303No Hit
CCGTAACTGCAACTGCGCCGGGGACCTTCGCCCAGGAGACCCACCATAAG13.0303030303030303No Hit
GAACTCAAACTCAGTGATTATGATGGACGACTAATCCAGAATAGCATAAC13.0303030303030303No Hit
CTCTCCCACTCACTGGATGTTCTTTCCCTTCTCATCCTGAATGGTGGGGG13.0303030303030303No Hit
CACACACACACCTGCATGTATGGACACCCAAGGGCTGCATTCTTTCTTGT13.0303030303030303No Hit
GCCCGTGCTGACCGCCTCCATCGCCACCACCTATGCCATCCTCAAGGAAC13.0303030303030303No Hit
TGTGAGGACCAAATGAGATCAAGAATGCAAAAGCTCTCTGTCAACTGTGA13.0303030303030303No Hit
GGGCAGACCGTGCCAGAAAGATGAGAGCTTCAATTTCAGCATTCCCAGGA13.0303030303030303No Hit
GTTTTCTCCACCCCGAACACGGACCCTCAGCGCCCAGCACTCGGAAGGCT13.0303030303030303No Hit
GATCTGCCCGCTCAGTTGGTCTTTCCATGTTACACTTTCCACTTTTTGAG13.0303030303030303No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)