FastQCFastQC Report
Sat 14 May 2022
HT5MLBGXL_n01_New_FCC_PCR_Neg_rep2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHT5MLBGXL_n01_New_FCC_PCR_Neg_rep2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences166535
Sequences flagged as poor quality0
Sequence length151
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TATTAAAACTGGGAGTGACAGAAGAAAGAAAAGCAAGAATAGAGACCCTA50893.0558140931335758No Hit
CATTATAAGGACCCCCAGGAAATCTCCCGTCCAGTATCAGCACCCAGAAA42242.5364037589695863No Hit
CATAAAATAGAACATCATAGCATATTAAAACTGGGAGTGACAGAAGAAAG36962.219353289098388No Hit
AAATAGAACATCATAGCATATTAAAACTGGGAGTGACAGAAGAAAGAAAA33972.0398114510463268No Hit
CCCACATAGTGTCCTCATTATAAGGACCCCCAGGAAATCTCCCGTCCAGT29201.7533851742876874No Hit
TCATAGCATATTAAAACTGGGAGTGACAGAAGAAAGAAAAGCAAGAATAG28941.7377728405440298No Hit
GGTGAGGAAGGCCCCCACATAGTGTCCTCATTATAAGGACCCCCAGGAAA24551.4741645900261207No Hit
CCCCCACATAGTGTCCTCATTATAAGGACCCCCAGGAAATCTCCCGTCCA22441.3474644969525926No Hit
ACATAAAATAGAACATCATAGCATATTAAAACTGGGAGTGACAGAAGAAA21811.3096346113429609No Hit
CATCATAGCATATTAAAACTGGGAGTGACAGAAGAAAGAAAAGCAAGAAT17401.0448254120755398No Hit
AAGCAGGACACATAAAATAGAACATCATAGCATATTAAAACTGGGAGTGA17001.0208064370852974No Hit
CACATAGTGTCCTCATTATAAGGACCCCCAGGAAATCTCCCGTCCAGTAT16380.9835770258504218No Hit
AGCGAAAGCAGGACACATAAAATAGAACATCATAGCATATTAAAACTGGG13080.785420482180923No Hit
TTATAAGGACCCCCAGGAAATCTCCCGTCCAGTATCAGCACCCAGAAAGG11800.7085597622121476No Hit
AGCGAAAGCAGGTGAGGAAGGCCCCCACATAGTGTCCTCATTATAAGGAC9630.5782568228900832No Hit
ACCCTAAGCAGAGGCAGGAATGAGGCTAAAATTGCAGACCAGAACGACCC9120.5476326297775242No Hit
CATAGTGTCCTCATTATAAGGACCCCCAGGAAATCTCCCGTCCAGTATCA9020.5416278860299636No Hit
ATAGAACATCATAGCATATTAAAACTGGGAGTGACAGAAGAAAGAAAAGC8510.5110036929174048No Hit
ATAGTGTCCTCATTATAAGGACCCCCAGGAAATCTCCCGTCCAGTATCAG8230.4941904104242351No Hit
GTATCAGCACCCAGAAAGGAAGACCTCCCCAGGAGCTAACTGGGGACTTT8230.4941904104242351No Hit
ATTATAAGGACCCCCAGGAAATCTCCCGTCCAGTATCAGCACCCAGAAAG8220.4935899360494791No Hit
GTCCAGTATCAGCACCCAGAAAGGAAGACCTCCCCAGGAGCTAACTGGGG8140.4887861410514306No Hit
CCCTAAGCAGAGGCAGGAATGAGGCTAAAATTGCAGACCAGAACGACCCT8010.48097997417960187No Hit
ACATCATAGCATATTAAAACTGGGAGTGACAGAAGAAAGAAAAGCAAGAA7770.4665685891854565No Hit
GAACATCATAGCATATTAAAACTGGGAGTGACAGAAGAAAGAAAAGCAAG7440.44675293481850664No Hit
GCCCCCACATAGTGTCCTCATTATAAGGACCCCCAGGAAATCTCCCGTCC7240.4347434473233855No Hit
GGCCCCCACATAGTGTCCTCATTATAAGGACCCCCAGGAAATCTCCCGTC6740.4047197285855826No Hit
CCCCAGGAGCTAACTGGGGACTTTTCACAATATGGACAGAAGGTGCTGGT6710.4029183054613144No Hit
AACTGGGAGTGACAGAAGAAAGAAAAGCAAGAATAGAGACCCTAAGCAGA6130.3680907917254631No Hit
GTCCTCATTATAAGGACCCCCAGGAAATCTCCCGTCCAGTATCAGCACCC6110.366889842975951No Hit
GTGTCCTCATTATAAGGACCCCCAGGAAATCTCCCGTCCAGTATCAGCAC5850.3512775092322935No Hit
CCCCCAGGAAATCTCCCGTCCAGTATCAGCACCCAGAAAGGAAGACCTCC5810.3488756117332693No Hit
ACATAGTGTCCTCATTATAAGGACCCCCAGGAAATCTCCCGTCCAGTATC5750.3452727654847329No Hit
GAAGAAAGAAAAGCAAGAATAGAGACCCTAAGCAGAGGCAGGAATGAGGC5740.3446722911099769No Hit
GAATAGAGACCCTAAGCAGAGGCAGGAATGAGGCTAAAATTGCAGACCAG5500.33026090611583153No Hit
GGAAATCTCCCGTCCAGTATCAGCACCCAGAAAGGAAGACCTCCCCAGGA5440.3266580598672952No Hit
GGACACATAAAATAGAACATCATAGCATATTAAAACTGGGAGTGACAGAA5330.32005284174497856No Hit
GAAAGCAGGTGAGGAAGGCCCCCACATAGTGTCCTCATTATAAGGACCCC5300.3182514186207104No Hit
GAGATGGAGATGGCCATGAGAATGCAAGTTGTGCATTTGAAAGTCACAAA5230.31404809799741795No Hit
CCTCATTATAAGGACCCCCAGGAAATCTCCCGTCCAGTATCAGCACCCAG5170.3104452517488816No Hit
AAAGCAGGCAGAGAGGCAGGCAGAGAGAGAGGAGGAAGCAGGCTCCCCGC5140.3086438286246134No Hit
CAGGTGAGGAAGGCCCCCACATAGTGTCCTCATTATAAGGACCCCCAGGA5100.3062419311255892No Hit
GGCAGAGAGAGAGGAGGAAGCAGGCTCCCCGCTGAGCAGAGAGCCCGATG5020.3014381361275408No Hit
CTTCCACTCTCACCCCTTACCTCCTTTTGTCCCCTTACTTTCACACTCCT4980.2990362386285165No Hit
ATATTAAAACTGGGAGTGACAGAAGAAAGAAAAGCAAGAATAGAGACCCT4920.2954333923799802No Hit
CTCTCACCCCTTACCTCCTTTTGTCCCCTTACTTTCACACTCCTTGCCAT4800.28822769988290753No Hit
CTCCTTGCCATTGCCAACATTCTTTGCCCCACTTGGAAAAGTCTCTCTCA4760.2858258023838833No Hit
CAAGAATAGAGACCCTAAGCAGAGGCAGGAATGAGGCTAAAATTGCAGAC4680.2810220073858348No Hit
GGCCATGAGAATGCAAGTTGTGCATTTGAAAGTCACAAAGGTCCACAAAG4610.2768186867625424No Hit
CACATAAAATAGAACATCATAGCATATTAAAACTGGGAGTGACAGAAGAA4550.27321584051400605No Hit
GCATAAAGAGTACTAGTCCCTGGCCCTGCTCTCACTAACTGGAAGAAATT4500.2702134686402258No Hit
GAAATCTCCCGTCCAGTATCAGCACCCAGAAAGGAAGACCTCCCCAGGAG4430.2660101480169334No Hit
GAAAGGAAGACCTCCCCAGGAGCTAACTGGGGACTTTTCACAATATGGAC4360.261806827393641No Hit
GAGGAAGGCCCCCACATAGTGTCCTCATTATAAGGACCCCCAGGAAATCT4210.2527997117723001No Hit
AAATAATACAGAAGTGTTTAGCATAAAGAGTACTAGTCCCTGGCCCTGCT4080.2449935449004714No Hit
ATAAGGACCCCCAGGAAATCTCCCGTCCAGTATCAGCACCCAGAAAGGAA4050.2431921217762032No Hit
GCAGGAAGGAGCTCAGAGATGGAGATGGCCATGAGAATGCAAGTTGTGCA3960.23778785240339867No Hit
GTACTAGTCCCTGGCCCTGCTCTCACTAACTGGAAGAAATTGCCCTTGTA3930.23598642927913052No Hit
ATTAAAACTGGGAGTGACAGAAGAAAGAAAAGCAAGAATAGAGACCCTAA3890.2335845317801063No Hit
GGCAGAGAGGCAGGCAGAGAGAGAGGAGGAAGCAGGCTCCCCGCTGAGCA3830.22998168553156995No Hit
ATACTAAGGAGGTCACTTTCTGTGATGAGCACTGGGTATTACATGTTAGT3830.22998168553156995No Hit
ATAGAGACCCTAAGCAGAGGCAGGAATGAGGCTAAAATTGCAGACCAGAA3700.22217551865974122No Hit
TTAAAACTGGGAGTGACAGAAGAAAGAAAAGCAAGAATAGAGACCCTAAG3520.21136697991413217No Hit
CTCCCGTCCAGTATCAGCACCCAGAAAGGAAGACCTCCCCAGGAGCTAAC3510.21076650553937612No Hit
TTCACACTCCTTGCCATTGCCAACATTCTTTGCCCCACTTGGAAAAGTCT3510.21076650553937612No Hit
AAGCAGGTGAGGAAGGCCCCCACATAGTGTCCTCATTATAAGGACCCCCA3430.20596271054132767No Hit
AAGCAGAGGCAGGAATGAGGCTAAAATTGCAGACCAGAACGACCCTTGTA3420.20536223616657157No Hit
ATGTAGATGAAGACACAACATTCATTTTGGAAGACAGGAAGATATAGAAC3290.19755606929474284No Hit
CCTTTTGTCCCCTTACTTTCACACTCCTTGCCATTGCCAACATTCTTTGC3240.19455369742096257No Hit
CCCGTAAGAGACTCTAACTACAGACAATAAACTTAGCGCTGGTGGAGGGG3220.19335274867145044No Hit
CCCCTTACCTCCTTTTGTCCCCTTACTTTCACACTCCTTGCCATTGCCAA3190.19155132554718227No Hit
GCAGGACACATAAAATAGAACATCATAGCATATTAAAACTGGGAGTGACA3180.19095085117242622No Hit
CAATATGGACAGAAGGTGCTGGTAGCTGACCAGGCTTCCCTTTTCCCCAG3160.1897499024229141No Hit
GCATATTAAAACTGGGAGTGACAGAAGAAAGAAAAGCAAGAATAGAGACC3160.1897499024229141No Hit
CCCAGAAAGGAAGACCTCCCCAGGAGCTAACTGGGGACTTTTCACAATAT3100.18614705617437777No Hit
GCAAGAATAGAGACCCTAAGCAGAGGCAGGAATGAGGCTAAAATTGCAGA3100.18614705617437777No Hit
CCACATAGTGTCCTCATTATAAGGACCCCCAGGAAATCTCCCGTCCAGTA3080.18494610742486564No Hit
CTTTCACACTCCTTGCCATTGCCAACATTCTTTGCCCCACTTGGAAAAGT3010.18074278680157324No Hit
GGGAGTGACAGAAGAAAGAAAAGCAAGAATAGAGACCCTAAGCAGAGGCA3000.1801423124268172No Hit
GCTCTAGGGGGGTCCCAAGCCAGCAGCCACTGGGGAAAAGGGAAGCCTGG2970.17834088930254902No Hit
GCGAAAGCAGGTGAGGAAGGCCCCCACATAGTGTCCTCATTATAAGGACC2960.17774041492779297No Hit
AGAATAGAGACCCTAAGCAGAGGCAGGAATGAGGCTAAAATTGCAGACCA2930.17593899180352476No Hit
ATATAGAACAGATAACAGGGACACAGAGAGGGAGGGATAGCTCGTGGGAG2930.17593899180352476No Hit
ATATATATCATGCTAACACAAATCATAAGAAAACTGAGTGTCTGGGGTGC2850.17113519680547634No Hit
CTCTAGAAGAACTATAAACATTAAAAAAAAGTTTTACAGCCCTCTTCTCA2820.16933377368120814No Hit
ATACAGAAGTGTTTAGCATAAAGAGTACTAGTCCCTGGCCCTGCTCTCAC2790.16753235055693996No Hit
CAATAAACTTAGCGCTGGTGGAGGGGTGGTGGGTGGAGGGATGAGCTAAA2780.1669318761821839No Hit
GGCAGGAATGAGGCTAAAATTGCAGACCAGAACGACCCTTGTATACGCTT2770.16633140180742786No Hit
GTTTAGCATAAAGAGTACTAGTCCCTGGCCCTGCTCTCACTAACTGGAAG2750.16513045305791577No Hit
CTTTTAGCTCTAGGGGGGTCCCAAGCCAGCAGCCACTGGGGAAAAGGGAA2750.16513045305791577No Hit
GGAGCTAACTGGGGACTTTTCACAATATGGACAGAAGGTGCTGGTAGCTG2700.16212808118413546No Hit
GTATTATTTTGTGATTTATTTATTAATTGTTAAACAATTATGGAAATACA2690.16152760680937941No Hit
GCGAAAGCAGGACACATAAAATAGAACATCATAGCATATTAAAACTGGGA2650.1591257093103552No Hit
CCCCACATAGTGTCCTCATTATAAGGACCCCCAGGAAATCTCCCGTCCAG2630.15792476056084306No Hit
AAAGCAGACCCAGTGAGAAACAGCAAAAATAATTTGAAAACGTTTTTCTC2590.15552286306181884No Hit
GGACAGAAGGTGCTGGTAGCTGACCAGGCTTCCCTTTTCCCCAGTGGCTG2590.15552286306181884No Hit
TCCCAGGACCCTGAGATCATGATCTGAGCCGAAGGCAGCGGCTTAACCCA2570.1543219143123067No Hit
GACCAGATTTGTCCTGCTGGCCATGTTTTACTGACCCATCCTCTAGAAGA2570.1543219143123067No Hit
GATTTATTATGAGCATATATTTCTTTTGTAATTAAAAAAGGAATGAAAGT2570.1543219143123067No Hit
GACCCTAAGCAGAGGCAGGAATGAGGCTAAAATTGCAGACCAGAACGACC2560.15372143993755066No Hit
GCTCAGAGATGGAGATGGCCATGAGAATGCAAGTTGTGCATTTGAAAGTC2540.15252049118803854No Hit
AAAGCAGGCTTCCCCTTCCACTCTCACCCCTTACCTCCTTTTGTCCCCTT2480.14891764493950221No Hit
GATGAAGACACAACATTCATTTTGGAAGACAGGAAGATATAGAACAGATA2470.14831717056474614No Hit
AGCGAAAGCAGGCAGAGAGGCAGGCAGAGAGAGAGGAGGAAGCAGGCTCC2470.14831717056474614No Hit
AAAGCAGGCAGCAGACCAGATTTGTCCTGCTGGCCATGTTTTACTGACCC2450.14711622181523404No Hit
CTCCCACCCCCATGTCCCACAGAAGTTTGTGTGTGTGTTTCTTTTCCAAA2450.14711622181523404No Hit
GCCCTAAAGAGATAGCGAGATCTCAACTGATACCTGCAAACACTTCCCCA2430.1459152730657219No Hit
GTATACGCTTTTAGCTCTAGGGGGGTCCCAAGCCAGCAGCCACTGGGGAA2420.14531479869096586No Hit
AGCAAGAATAGAGACCCTAAGCAGAGGCAGGAATGAGGCTAAAATTGCAG2380.14291290119194164No Hit
CCCTATATGTTAATAAATAGAATTTAAATAAAAGTGTGAAAAAAAGAAAA2380.14291290119194164No Hit
TGACAGAAGAAAGAAAAGCAAGAATAGAGACCCTAAGCAGAGGCAGGAAT2370.1423124268171856No Hit
AAGGAAGACCTCCCCAGGAGCTAACTGGGGACTTTTCACAATATGGACAG2320.1393100549434053No Hit
ACTTATGATTTATTATGAGCATATATTTCTTTTGTAATTAAAAAAGGAAT2280.13690815744438106No Hit
TTACTGACCCATCCTCTAGAAGAACTATAAACATTAAAAAAAAGTTTTAC2270.136307683069625No Hit
CTCTAACTACAGACAATAAACTTAGCGCTGGTGGAGGGGTGGTGGGTGGA2250.1351067343201129No Hit
CTCAAGCACTCTGCTTATTCAAATCCTGCCTGTGCTTTAGGGCACAACCT2250.1351067343201129No Hit
GGAAGGCCCCCACATAGTGTCCTCATTATAAGGACCCCCAGGAAATCTCC2240.13450625994535684No Hit
ATAAAAGGGTTTGTCCCACAAGTAGGAAGATAATGCAATAATAGCTGAGA2230.1339057855706008No Hit
GTATTACATGTTAGTGATGAATCACTAAATTCTACTCCGGAAACCAATAA2210.13270483682108866No Hit
GACCAGAACGACCCTTGTATACGCTTTTAGCTCTAGGGGGGTCCCAAGCC2200.13210436244633258No Hit
ATAATACAGAAGTGTTTAGCATAAAGAGTACTAGTCCCTGGCCCTGCTCT2140.12850151619779626No Hit
ATCATGATCTGAGCCGAAGGCAGCGGCTTAACCCACTGAGCCACCCAGGC2140.12850151619779626No Hit
GTATAAGACTTTGGGCCAGTCATTTAAGCGCTCTTGGCCTTTGAGGTAAT2140.12850151619779626No Hit
ACCCGTAAGAGACTCTAACTACAGACAATAAACTTAGCGCTGGTGGAGGG2140.12850151619779626No Hit
CATATAGGGTATTATTGGTTTCCGGAGTAGAATTTAGTGATTCATCACTA2140.12850151619779626No Hit
CTCTAGGGGGGTCCCAAGCCAGCAGCCACTGGGGAAAAGGGAAGCCTGGT2090.12549914432401596No Hit
TGTATACGCTTTTAGCTCTAGGGGGGTCCCAAGCCAGCAGCCACTGGGGA2090.12549914432401596No Hit
GATTTGTCCTGCTGGCCATGTTTTACTGACCCATCCTCTAGAAGAACTAT2090.12549914432401596No Hit
AGCGAAAGCAGGGAAGGAAGGAAGGAAATCGAAAGCTAACGATGTAGATG2060.1236977211997478No Hit
ATCCCAGGACCCTGAGATCATGATCTGAGCCGAAGGCAGCGGCTTAACCC2030.12189629807547962No Hit
ACCTCCTTTTGTCCCCTTACTTTCACACTCCTTGCCATTGCCAACATTCT2030.12189629807547962No Hit
GAATGCAAGTTGTGCATTTGAAAGTCACAAAGGTCCACAAAGTTCTGTGG2010.12069534932596751No Hit
CCCTTCCACTCTCACCCCTTACCTCCTTTTGTCCCCTTACTTTCACACTC2000.12009487495121146No Hit
AGCAGGCAGAGAGGCAGGCAGAGAGAGAGGAGGAAGCAGGCTCCCCGCTG1970.11829345182694329No Hit
CCCAGGAAATCTCCCGTCCAGTATCAGCACCCAGAAAGGAAGACCTCCCC1970.11829345182694329No Hit
GCAGAGAGGCAGGCAGAGAGAGAGGAGGAAGCAGGCTCCCCGCTGAGCAG1960.11769297745218724No Hit
GAGGCAGGCAGAGAGAGAGGAGGAAGCAGGCTCCCCGCTGAGCAGAGAGC1950.11709250307743116No Hit
ATTATGAGCATATATTTCTTTTGTAATTAAAAAAGGAATGAAAGTTTGGA1940.11649202870267511No Hit
GGCTAAAATTGCAGACCAGAACGACCCTTGTATACGCTTTTAGCTCTAGG1940.11649202870267511No Hit
CCCTTGTATACGCTTTTAGCTCTAGGGGGGTCCCAAGCCAGCAGCCACTG1940.11649202870267511No Hit
GAGTAGAAGGCAGTAAATGGCTAGCTCAGTGAGTGTCCCAATTGGATTTA1930.11589155432791905No Hit
ATATTAAATAAATTACTTATGATTTATTATGAGCATATATTTCTTTTGTA1910.11469060557840695No Hit
GACAGAAGGTGCTGGTAGCTGACCAGGCTTCCCTTTTCCCCAGTGGCTGC1900.11409013120365087No Hit
GTGGAGGGATGAGCTAAATGGGTGATGGATACTAAGGAGGTCACTTTCTG1900.11409013120365087No Hit
TAGAGACCCTAAGCAGAGGCAGGAATGAGGCTAAAATTGCAGACCAGAAC1900.11409013120365087No Hit
GGGTATTACATGTTAGTGATGAATCACTAAATTCTACTCCGGAAACCAAT1890.11348965682889482No Hit
CAGTATCAGCACCCAGAAAGGAAGACCTCCCCAGGAGCTAACTGGGGACT1880.11288918245413877No Hit
AAAGAGTACTAGTCCCTGGCCCTGCTCTCACTAACTGGAAGAAATTGCCC1870.11228870807938271No Hit
GTGAGAAACAGCAAAAATAATTTGAAAACGTTTTTCTCTCACTAAGCTGT1870.11228870807938271No Hit
TATCAGCACCCAGAAAGGAAGACCTCCCCAGGAGCTAACTGGGGACTTTT1860.11168823370462666No Hit
GCAGGTGAGGAAGGCCCCCACATAGTGTCCTCATTATAAGGACCCCCAGG1810.10868586183084637No Hit
GGCTTCCCCTTCCACTCTCACCCCTTACCTCCTTTTGTCCCCTTACTTTC1810.10868586183084637No Hit
CCTTAAATTATCTCTGTCAATTTGAAGTAGACTTTCCCAGAAGTTTTAAC1810.10868586183084637No Hit
GTCCCTGGCCCTGCTCTCACTAACTGGAAGAAATTGCCCTTGTAGAATTC1790.10748491308133425No Hit
ACTGGGGACTTTTCACAATATGGACAGAAGGTGCTGGTAGCTGACCAGGC1780.1068844387065782No Hit
GACTAGTACTCTTTATGCTAAACACTTCTGTATTATTTTGTGATTTATTT1780.1068844387065782No Hit
GTAAATGGCTAGCTCAGTGAGTGTCCCAATTGGATTTAAGAGAGAAGGGA1770.10628396433182215No Hit
CAATTATGGAAATACACAAATGTTTATTTAATATTAAATAAATTACTTAT1750.10508301558231004No Hit
ACATAAAAGAGAGCTGTCCCTTCTCTCTTAAATCCAATTGGGACACTCAC1740.10448254120755396No Hit
GAGAGAGAGGAGGAAGCAGGCTCCCCGCTGAGCAGAGAGCCCGATGCGGG1740.10448254120755396No Hit
GAAGGAAGGAAGGAAATCGAAAGCTAACGATGTAGATGAAGACACAACAT1710.10268111808328578No Hit
ATGTTTTACTGACCCATCCTCTAGAAGAACTATAAACATTAAAAAAAAGT1700.10208064370852973No Hit
GAAATAAATAGTCCAAGTTTATCTTAATAGGCCCTAAAGAGATAGCGAGA1690.10148016933377368No Hit
GGGAAGGAAGGAAGGAAATCGAAAGCTAACGATGTAGATGAAGACACAAC1690.10148016933377368No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)