FastQCFastQC Report
Sat 14 May 2022
HT5MLBGXL_n01_New_FCC_1797_d06_rep2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHT5MLBGXL_n01_New_FCC_1797_d06_rep2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2494535
Sequences flagged as poor quality0
Sequence length151
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTCCAACACTGTGTCAAGCTTTCAGGTAGACTGCTTTCTTTGGCATGTCC136620.5476772224081843No Hit
GAGCAAAAGCAGGGTGACAAAAACATAATGGATTCCAACACTGTGTCAAG83120.33320839354829657No Hit
GTATAGGTGGGTGGGTGATGTATGCCCCACACGAAAAGAATGCTCTTTCC76640.3072316082957345No Hit
GAGCAAAAGCAGGTAGATGTTGAAAGATGAGTCTTCTAACCGAGGTCGAA72160.2892723493556915No Hit
GTTGTAAGGCTTGCATAAAGGTTATTTGCTCAAAGCTATTCTCTGTAATT62420.2502269962137232No Hit
GCTCTATGTTGACAAAATGACCATCGTCAACATCCACAGCATTCTGCTGT51440.206210776757993No Hit
CTATTGCTCCTTCATCTGTAAACGCTCTAAGTAGTATCAGAGCTTCAAGT46710.18724932702888514No Hit
GCAAAAGCAGGGTGACAAAAACATAATGGATTCCAACACTGTGTCAAGCT42590.17073322282509565No Hit
GTCCTACATCGTCGAAAGATCATCAGCTGTAAATGGAACGTGTTACCCTG42350.16977111966759334No Hit
TTCTTAACCTATGTCGCATTTCTTCAATTAACCATCTTATCTCTTCAAAC40510.16239499546007574No Hit
GTGTTATCATTCCATTCAAGTCCTCCGATGAGGACCCCAATTGCATTTTT39980.16027035098725814No Hit
GGCTAGTACCATTCTGTTCTCATGCCTGATTAGTGGGTTGGTGGTAGTCG37910.1519722112538008No Hit
AAGCTATTCTCTGTAATTCTTAACCTATGTCGCATTTCTTCAATTAACCA37160.1489656388866061No Hit
GTGTCAAGCTTTCAGGTAGACTGCTTTCTTTGGCATGTCCGCAAACGATT37040.14848458730785496No Hit
CACCTATACCGAGCAAACAAATTTGTACATAAGAAACGACACAACAACAA36940.148083710992229No Hit
GCATATAGTGGAGCGGATTCTGGAGGAAGAATCTGACGAGGCACTTAAAA35990.1442753859937824No Hit
GCATAAAGGTTATTTGCTCAAAGCTATTCTCTGTAATTCTTAACCTATGT35030.14042697336377322No Hit
CTCATGGAATGGCTAAAGACAAGACCAATCCTGTCACCTCTGACTAAGGG34130.1368190865231396No Hit
GTTTTAGTACCGACCAATAATAATCAATTCTTCCCTGCAGACCATTGACA33980.13621777204970065No Hit
GTGCAGACCGTGCCAGAAAGATGAGATCTTCAATTTCAGCATTCCCAGGA33790.13545610705001132No Hit
CTCTTGGTCTGGACATCAGAACTGCCACTCGTGAAGGAAAGCATATAGTG33630.1348147049450098No Hit
GTCCTGGAAGGGAAGGTAATGGTGAGATTTCGCCCACTATTGCTCCTTCA33220.13317111205094337No Hit
CTCATAGACTCAGGCACTCCTTCCGTAGAAGGCCCTCTTTTCAAACCGTA32100.12868129731593264No Hit
GTATAACAATAGAGAGAATGGTTCTCTCCGCATTTGATGAAAGGAGGAAC31010.12431174547560969No Hit
AAGTAGTATCAGAGCTTCAAGTCGATTAAAAATCACACTAAAGTTTGCTT30420.12194657521341654No Hit
GTACTAGCCAGCACTACGGCTAAGGCCATGGAGCAGATGGCTGGATCAAG30390.12182631231872874No Hit
GGTTAATGCTCATTCCCAAGCAGAAAGTGACAGGGCCCCTTTGCATTAGA30180.12098447205591424No Hit
GAACTAGGGTGAGTCCCAATTGTCCTCATAGCCTGCACCATTTGCCTAGC29770.1193408791618478No Hit
GTCATATTCCTCTGCATTGTCTCCGAAGAAATAAGATCCTTCATTACTCA29140.11681535837340426No Hit
CTCATATGCAATCCTTGTTCTTCGTCCATTATCGCCTCTCCAGAAGTTCC28780.11537220363715081No Hit
CTATCATCCCATCAGGCCCCCTCAAAGCCGAGATCGCGCAGAGACTTGAG28300.11344799732214621No Hit
CTATTAGTAGAGGTCTTATTTTCTCAATTTTCTTTCTCGTCGATTCGTTG28220.11312729626964545No Hit
CAACATGGACAGGGCAGTTAAACTATACAAGAAGCTGAAGAGGGAAATGA28010.11228545600683094No Hit
GTATGGATAGCAAATAGTAGCATTGCCACAACTATTTTAGTGCATGTGTG28000.11224536837526834No Hit
AGCAGGTACTGATCCAAAATGGAAGACTTTGTGCGACAGTGCTTCAATCC27510.11028107442870114No Hit
GCATATGAGAGAATGTGCAACATCCTCAAAGGGAAATTTCAAACAGCAGC27480.11016081153401334No Hit
GCTCCACACAGAGCATAATGGAATGCTGTGTGCAACAAGCCTGGGACATC27430.10996037337620039No Hit
GAGTGGAGATCTCCCATTCTCATCACTGCTTCTCCAAGTGAATCTCTGTA27090.1085973939030721No Hit
CTGTAATTCTTAACCTATGTCGCATTTCTTCAATTAACCATCTTATCTCT26770.10731458969306905No Hit
ACCCATTACTGCCTGGTCCATTCTAATGCAAAGGGGCCCTGTCACTTTCT26230.10514985758868889No Hit
CTATACTACTAGTAGTAACAGCAAGCAATGCAGATAAAATCTGCATCGGC26120.10470889364150031No Hit
TTCTTAGACCATGAACCAATACTGTCGGTAAGCATCCCATCATAAATGAT25780.10334591416837205No Hit
GTTCTTATCTCTTGCTCCACTTCAAGCAATAGTTGTAAGGCTTGCATAAA25560.10246398627399494No Hit
ACCTAACTGAAATGACTCTTGAGGAAATGTCAAGGGATTGGTTAATGCTC25450.10202302232680639No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)