FastQCFastQC Report
Thu 20 Jul 2017
HT5LJBCXY_l02n02_inf_cell95.35200000095b66.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHT5LJBCXY_l02n02_inf_cell95.35200000095b66.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1794518
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGATCTG19250.029.3344465
CTTCCGA34750.028.8745331
TTTCGCG406.1714585E-428.12191818
CCGATCT34650.028.11384
TCCGATC35000.027.9612223
TTCCGAT35500.027.757522
CGATCTA5200.026.8239825
CGGGTAT651.525665E-524.22811315
GACGTGT4300.019.88154210
GTACTAG706.9907156E-419.28361
CGATCTT4700.019.146845
GATCTGG13350.018.7058166
GTATATA5700.018.5505981
CGATCTC10550.017.6994825
GTATTAA4500.017.4980831
ATCTGCG3100.017.4174467
GGGTGTA4050.017.22033515
GAGACGT5800.017.0670958
TCTTCCG15100.016.6869135
GATCTGT7100.016.16026
Produced by FastQC (version 0.11.2)