FastQCFastQC Report
Thu 20 Jul 2017
HT5LJBCXY_l02n02_inf_cell94.35200000095b5a.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHT5LJBCXY_l02n02_inf_cell94.35200000095b5a.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1377370
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCCTATACCTTCTGCATAATGAATTAACTAGAAATAACTTTGCAAGGAGA19300.14012211678779124No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGATCTG15050.031.6893655
CTTCCGA28300.031.2406671
TCCGATC28200.031.0323523
CCGATCT28600.030.5196744
TTCCGAT28850.030.411162
CGATCTA4400.029.143145
CTGCGTA751.7003804E-623.9962259
CGATCTC7900.023.6355235
CGCCTAT802.9739022E-622.50136234
ATCTGCG3000.021.746587
GCGTCTA550.003935549420.45578234
CGATCTT4550.019.282685
TCTTCCG10300.019.2202765
CGGTACT1951.8189894E-1218.46533838
GATCTAC3100.018.1423076
GATCTGC8200.018.1069076
GGGGTTA2350.017.231336
GCTCTTC11400.017.1683523
TAACGCC1459.688847E-817.066284
ATAACGC1752.6975613E-916.7116573
Produced by FastQC (version 0.11.2)