FastQCFastQC Report
Thu 20 Jul 2017
HT5LJBCXY_l02n02_inf_cell93.35200000095b23.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHT5LJBCXY_l02n02_inf_cell93.35200000095b23.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences145664
Sequences flagged as poor quality0
Sequence length51
%GC48

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCGT250.002099038336.00714532
GAATGGG250.002102580935.994786
ATCGTGC300.005127702430.0059534
TCGCAAG300.005136323629.99565123
ACCGGGT300.005136323629.99565114
GGCCGTG300.005136323629.99565111
TCAGGAT300.005136323629.9956512
GGGAACC300.005136323629.99565112
TGAATGG300.005136323629.9956515
GTGCGAT406.145322E-428.12092419
GGAGACG1200.028.1209227
AGTGCGA507.123315E-526.99608618
GAGACGT1251.8189894E-1225.1963468
TGTCGGG500.002252597122.50446531
TGCTCGA500.002257099422.49673812
ACGGTCC500.002257099422.4967386
GGGAGAC1700.021.17346
TTGTCGG550.003915799320.45860330
GTCGGGT550.003915799320.45860332
ACGTGTG1651.8189894E-1220.45158211
Produced by FastQC (version 0.11.2)