FastQCFastQC Report
Thu 20 Jul 2017
HT5LJBCXY_l02n02_inf_cell92.35200000095b09.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHT5LJBCXY_l02n02_inf_cell92.35200000095b09.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences944435
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCCTATACCTTCTGCATAATGAATTAACTAGAAATAACTTTGCAAGGAGA14530.1538485973095025No Hit
TCTATAGGGTGATAGATTGGTCCAATTGGGTGTGAGGAGTTCAGTTATATG9690.10260102601026011No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCCTAG406.1677425E-428.12311416
TCCGTTC406.1677425E-428.12311422
TCCGATC21950.027.3671843
CGATCTG11600.027.3473035
CTTCCGA22300.027.3412171
TTCCGAT22100.027.2832362
CCGATCT22550.026.639014
ATCTGCG1600.025.3108027
GACGTGT5500.023.31661810
GAGACGT5700.022.8932048
ACGTGTG5900.022.1171611
CGATCTT2650.022.073995
CGATCTA4100.021.9497475
CGATCTC6700.021.1552985
TATACTG1301.2278178E-920.7678385
CGGGTAT550.00393755520.45317515
AGACGTG6050.020.4531739
GGGAGAC6900.020.2160386
GTCGACG600.006498256718.75569343
CGTCGAC600.006498256718.75569342
Produced by FastQC (version 0.11.2)