FastQCFastQC Report
Thu 20 Jul 2017
HT5LJBCXY_l02n02_inf_cell91.35200000095aee.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHT5LJBCXY_l02n02_inf_cell91.35200000095aee.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1804358
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGATCTA5950.026.4653055
CCGATCT31900.025.4573024
TTCCGAT31800.025.3958762
TCCGATC31950.025.3470543
CTTCCGA33200.024.731511
GACGTGT9950.024.64400710
CGATCTG14150.024.3237675
GAGACGT10650.023.2347988
CGATCTT5950.022.6845475
ACGTGTG10550.022.60275311
ACGCGTG903.2769822E-722.5086140
AGACGTG11100.021.48229
GGAGACG12750.020.2900687
GACGCGT1009.039595E-720.2549439
CGTGTGC12650.019.02887712
GGGAGAC15050.017.6376766
CGGTACC1551.0844815E-817.42795245
CGAACTA1104.6422927E-516.36263824
TTAACGG1401.2553737E-616.07178535
CGATCTC11350.015.8558665
Produced by FastQC (version 0.11.2)