FastQCFastQC Report
Thu 20 Jul 2017
HT5LJBCXY_l02n02_inf_cell90.35200000095ac4.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHT5LJBCXY_l02n02_inf_cell90.35200000095ac4.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1694433
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCCTATACCTTCTGCATAATGAATTAACTAGAAATAACTTTGCAAGGAGA29360.17327330145246228No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTCCGA34000.031.232141
TCCGATC34050.031.1862773
CGATCTA5050.031.1849695
TTCCGAT34050.031.0541322
CGATCTG18850.031.031355
CCGATCT35050.030.1681374
CGTTAGG500.002266379322.4977289
CGATCTC10100.021.829485
GATCTGG12700.021.7891396
GTCTAAT2000.021.372841
ATCTGCG3800.020.1295477
ACCGTTA908.262248E-619.997988
GGTACTA4400.019.94646639
GATCTGC8650.019.50676
TGCGGTA5100.018.97091136
CGATCTT5150.018.7845125
GATCTCG5400.018.3314826
GATCTAG4050.018.3314826
CGGTACT5000.017.55185338
TCGAGTA2105.456968E-1217.14618541
Produced by FastQC (version 0.11.2)