FastQCFastQC Report
Thu 20 Jul 2017
HT5LJBCXY_l02n02_inf_cell9.352000000950b1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHT5LJBCXY_l02n02_inf_cell9.352000000950b1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2570696
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCCTATACCTTCTGCATAATGAATTAACTAGAAATAACTTTGCAAGGAGA39390.15322698599912243No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGATCTA8750.032.9114275
CTTCCGA62350.031.897791
TCCGATC62500.031.8212363
TTCCGAT63050.031.4722862
CCGATCT63100.031.3403824
CGATCTG34150.030.2389555
CGATCTC18150.025.7828855
CGATCTT7650.024.9978285
GATCTCG9500.022.4980456
ATCTGCG5150.020.5321967
GATCTGG22150.019.7048346
GTATTAA4900.019.2840391
GATCTGC14500.018.6190726
GATCTAG6300.018.5698176
TCTTCCG21650.018.4972385
ATCTCGT2800.018.4805377
TCTATAC3550.018.3786833
GATCTGT9550.017.9042036
GCTCTTC24350.016.7233943
CGGTACC4250.016.41768345
Produced by FastQC (version 0.11.2)