FastQCFastQC Report
Thu 20 Jul 2017
HT5LJBCXY_l02n02_inf_cell89.35200000095aab.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHT5LJBCXY_l02n02_inf_cell89.35200000095aab.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1617631
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCCTATACCTTCTGCATAATGAATTAACTAGAAATAACTTTGCAAGGAGA19820.12252485270126501No Hit
CTCCTATTGTGACTGGGTGTATATTCTGGTAAGGGAGACTGCTGTTTATAG19530.1207321076314685No Hit
CAATATGTATAGGCTACCATGCGAACAATTCAACCGACACTGTTGACACAG18030.1114592883049348No Hit
ATGTATAGGCTACCATGCGAACAATTCAACCGACACTGTTGACACAGTACT17890.11059382516779166No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGATCTG15200.031.8214595
CTTCCGA31800.030.4893761
CCGATCT32400.029.9266094
TCCGATC32550.029.8578113
TTCCGAT34900.027.782872
CGATCTA5400.023.7468685
GACGTGT3950.022.2122610
CGATCTC10500.021.8542585
CGATCTT6150.021.2167135
GATCTGC7750.020.0296156
CGTCGTA1503.45608E-1019.49742910
CGGTACC1850.019.46953245
ACGTGTG4750.019.41849111
GTATTAG5000.019.3462521
GGTCGCA2800.019.28555532
CCGGTTA1302.7657734E-819.04360441
GCATTCG3350.018.80465122
CGGGATA600.006515414518.74810815
GTCGCAT2900.018.62053533
TCTTCCG11450.018.27275
Produced by FastQC (version 0.11.2)