FastQCFastQC Report
Thu 20 Jul 2017
HT5LJBCXY_l02n02_inf_cell88.35200000095a81.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHT5LJBCXY_l02n02_inf_cell88.35200000095a81.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2606822
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCCTATACCTTCTGCATAATGAATTAACTAGAAATAACTTTGCAAGGAGA54530.20918190808578416No Hit
TCTATAGGGTGATAGATTGGTCCAATTGGGTGTGAGGAGTTCAGTTATATG37290.14304774165631562No Hit
ATATAGTACCGCAAGGGAAAGATGAAAAATTATAACCAAGCATAATATAGC31240.11983940598936176No Hit
GCATAATATAGCAAGGACTAACCCCTATACCTTCTGCATAATGAATTAACT26900.10319078172579485No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGATCTA7200.031.8705565
CTTCCGA46150.030.905771
TTCCGAT45650.030.8500252
TCCGATC46000.030.5174853
CCGATCT46950.029.8520684
CGATCTG24800.029.7539165
CGATCTT7200.023.4342335
CGTCGTA3200.021.0908110
CGATCTC13300.020.4670725
TACCGTC3700.019.4567497
TCTTCCG16250.019.2434715
GATCTAC5250.018.4260046
ATACCGT3950.018.2253076
ACCGTCG3600.018.1224738
GTATTAG11350.018.0371321
CGCCTAT2250.018.0019836
GATCTGG17300.017.8154356
GTATTAA5700.017.7606831
TCGAGTA2057.4578566E-1116.46712141
GGCGTTA3150.016.43321642
Produced by FastQC (version 0.11.2)