FastQCFastQC Report
Thu 20 Jul 2017
HT5LJBCXY_l02n02_inf_cell85.35200000095a24.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHT5LJBCXY_l02n02_inf_cell85.35200000095a24.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1607593
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCCTATACCTTCTGCATAATGAATTAACTAGAAATAACTTTGCAAGGAGA46560.2896255457693583No Hit
TCTATAGGGTGATAGATTGGTCCAATTGGGTGTGAGGAGTTCAGTTATATG32200.2002994539040665No Hit
ATATAGTACCGCAAGGGAAAGATGAAAAATTATAACCAAGCATAATATAGC30480.18960022841602323No Hit
GCATAATATAGCAAGGACTAACCCCTATACCTTCTGCATAATGAATTAACT24270.15097104802023897No Hit
AGATATAGTACCGCAAGGGAAAGATGAAAAATTATAACCAAGCATAATATA22500.1399607985354502No Hit
CCCTATAGAAGAACTAATGTTAGTATAAGTAACATGAAAACATTCTCCTCC20580.12801747706042513No Hit
GATTTATAGGTAGAGGCGACAAACCTACCGAGCCTGGTGATAGCTGGTTGT17340.10786312207132029No Hit
GTGATAGATTGGTCCAATTGGGTGTGAGGAGTTCAGTTATATGTTTGGGAT17070.10618359248889489No Hit
GTTATATGTTTGGGATTTTTTAGGTAGTGGGTGTTGAGCTTGAACGCTTTC16870.10493949650191312No Hit
GTATTAGAGGCACCGCCTGCCCAGTGACACATGTTTAACGGCCGCGGTACC16530.10282453332404408No Hit
ATCTATCACCCTATAGAAGAACTAATGTTAGTATAAGTAACATGAAAACAT16430.1022024853305532No Hit
CTTCTATAGGGTGATAGATTGGTCCAATTGGGTGTGAGGAGTTCAGTTATA16160.1005229557481278No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGATCTA4650.029.5145785
CGATCTG14450.029.4275195
CTTCCGA29800.028.839871
TTCCGAT30100.028.402942
TCCGATC30550.027.7636363
CCGATCT30450.027.41154
CGATCTT4300.024.0685025
TCGGTAA500.002265282222.49951727
GAGACGT3850.021.0378578
GACGTGT3550.020.91439410
ATCTGCG2050.020.8525647
CGGTAAG550.00393769920.45410728
ACGTGTG3850.020.45347211
CGGTACC4100.020.3101345
GCTCTTC11750.019.7217543
GATCTGC7000.018.6418786
GATCTGG9550.018.3759986
TCTTCCG12650.018.141345
TTAACGG4600.018.09743935
CGATCTC9550.017.9048185
Produced by FastQC (version 0.11.2)