FastQCFastQC Report
Thu 20 Jul 2017
HT5LJBCXY_l02n02_inf_cell84.35200000095a0a.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHT5LJBCXY_l02n02_inf_cell84.35200000095a0a.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2113821
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCCTATACCTTCTGCATAATGAATTAACTAGAAATAACTTTGCAAGGAGA35230.16666501089732763No Hit
ATATAGTACCGCAAGGGAAAGATGAAAAATTATAACCAAGCATAATATAGC22490.10639500695659662No Hit
TCTATAGGGTGATAGATTGGTCCAATTGGGTGTGAGGAGTTCAGTTATATG21390.10119116046249894No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGATCTA8250.027.8138395
TCCGATC47900.027.0045413
TTCCGAT48000.026.9482782
CTTCCGA49400.026.5033321
CCGATCT48800.026.3682124
CGATCTG24200.026.3078775
GACGTGT9450.024.5199810
GAGACGT9950.023.7400138
ATCCGTA602.4710695E-422.49648712
ACGTGTG10000.022.27152311
CGATCTC13900.020.716195
AGACGTG11250.019.9968769
GGAGACG13600.019.188187
GGGAGAC13750.018.8152456
CGATCTT7200.018.7470745
CGTGTGC13000.018.6893912
CGCTAAA851.2056163E-418.52827322
ATCTGCG3400.018.526527
GATCTCG6300.018.2114436
CGTCGAC750.00110828218.00570942
Produced by FastQC (version 0.11.2)