FastQCFastQC Report
Thu 20 Jul 2017
HT5LJBCXY_l02n02_inf_cell83.352000000959ef.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHT5LJBCXY_l02n02_inf_cell83.352000000959ef.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2285605
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GACGTGT17850.024.19833810
TTCCGAT50050.024.0476282
GAGACGT19450.023.711388
TCCGATC50900.023.6460483
CTTCCGA52950.023.1129551
CCGATCT52000.023.102584
ACGTGTG18600.023.1016511
CGATCTG25800.022.9328785
CGATCTA8400.022.4968935
GGAGACG21400.022.3917687
AGACGTG19450.021.9764029
GGGAGAC23150.021.4765156
CGTGTGC21800.021.0521412
GGCGTTA2600.019.9098142
CGATCTT8900.018.7052825
GCGAACT1454.780304E-918.62056428
GCATTCG5650.017.12302822
GTGTGCT25650.017.01519613
TGTGCTC25400.016.91695614
ATTCGTA5650.016.72481724
Produced by FastQC (version 0.11.2)