FastQCFastQC Report
Thu 20 Jul 2017
HT5LJBCXY_l02n02_inf_cell82.352000000959d2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHT5LJBCXY_l02n02_inf_cell82.352000000959d2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2381390
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCCTATACCTTCTGCATAATGAATTAACTAGAAATAACTTTGCAAGGAGA25450.10687035722834143No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGATCTA8900.026.5407585
TCCGATC50650.025.7165763
TTCCGAT50500.025.7038672
CCGATCT51400.025.2100324
CTTCCGA53450.024.36941
CGATCTG24150.024.1266295
CGATCTT8650.022.626495
GACGTGT15150.021.8282410
ACGTGTG15350.020.95804611
GAGACGT16900.020.7659558
GGAGACG18400.019.8066587
TACGCAA807.5765216E-519.68439710
AGACGTG17250.019.1708899
CGTGTGC18100.018.89244512
GGGAGAC20050.018.8498956
ATCTGCG3150.017.8543287
CGATCTC16250.017.7202845
TTAACGG2350.017.23530635
TCGTAGC2057.4578566E-1116.46116414
TATTACG1257.644494E-616.1974454
Produced by FastQC (version 0.11.2)