FastQCFastQC Report
Thu 20 Jul 2017
HT5LJBCXY_l02n02_inf_cell81.352000000959b8.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHT5LJBCXY_l02n02_inf_cell81.352000000959b8.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2235472
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGATCTG22850.027.0759475
TCCGATC52000.026.4780063
CCGATCT51850.026.4678254
TTCCGAT52300.026.2831082
CTTCCGA53850.025.6931231
CGATCTA8600.024.0672555
GAGACGT14150.024.0080958
CGATCTT9250.021.8896065
GACGTGT14600.021.5730910
GGAGACG17400.020.5581997
ACGTGTG15250.020.35853211
CGATCTC17300.020.2868985
AGACGTG15500.020.1753149
GGGAGAC18400.019.9299836
CGTGTGC18150.018.34519212
ACCGTCG3150.017.855288
TACCGTC2850.017.366617
GATCTGC11250.016.9982266
CGCTACG800.001712723816.8732410
GATCTGG14350.016.148146
Produced by FastQC (version 0.11.2)