FastQCFastQC Report
Thu 20 Jul 2017
HT5LJBCXY_l02n02_inf_cell80.3520000009599f.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHT5LJBCXY_l02n02_inf_cell80.3520000009599f.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1642378
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCCTATACCTTCTGCATAATGAATTAACTAGAAATAACTTTGCAAGGAGA19820.12067867445862038No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGCGTG551.1264456E-732.73314340
GACGCGT554.1605563E-628.64149939
CGATCTA5100.028.231765
CGATCTG16600.027.7826675
TTCCGAT34000.026.6657812
TCCGATC34150.026.5486533
CTTCCGA34650.026.3595331
CCGATCT34650.026.0357024
CGGGTAT709.3193194E-725.71185115
GAGACGT9200.025.4323738
GACGTGT8750.024.16913810
ACGTGTG8900.022.75065411
GGAGACG10850.022.1861637
AGACGTG9900.021.5888639
GGGAGAC11800.020.5906436
CGTGTGC10200.020.51276212
CGATCTC10800.019.1642675
GTATTAG6900.018.91011
CGGTACC2150.018.84288245
TTAACGG2150.018.83829335
Produced by FastQC (version 0.11.2)