FastQCFastQC Report
Thu 20 Jul 2017
HT5LJBCXY_l02n02_inf_cell8.35200000095098.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHT5LJBCXY_l02n02_inf_cell8.35200000095098.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1638202
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCCTATACCTTCTGCATAATGAATTAACTAGAAATAACTTTGCAAGGAGA18980.11585872804452685No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGATCTG17350.030.3413775
CTTCCGA34700.029.3688961
TCCGATC35250.028.6553753
TTCCGAT35750.028.25462
CCGATCT35900.028.1992114
CGCGAAT802.9758794E-622.50013728
CGATCTT6550.021.638055
CGATCTA6300.021.4254325
GACGTGT3700.021.28066610
CGATCTC10300.020.5309725
ATCTGCG2700.019.9970687
CGGGTAT706.992096E-419.28288815
TCTTCCG13900.019.0979215
GTCTATA1951.8189894E-1218.4588341
ACGTGTG4400.018.40639311
TAACCGT1507.2850526E-917.9973647
GGGGTTA2500.017.9973646
GGTACTA1902.3646862E-1117.76706339
GCTCTTC15450.017.1819483
GATCTGC8950.017.0924686
Produced by FastQC (version 0.11.2)