FastQCFastQC Report
Thu 20 Jul 2017
HT5LJBCXY_l02n02_inf_cell79.35200000095975.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHT5LJBCXY_l02n02_inf_cell79.35200000095975.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2305215
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCCTATACCTTCTGCATAATGAATTAACTAGAAATAACTTTGCAAGGAGA27940.12120344523178965No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGATCTG20100.027.1962095
TTCCGAT47550.027.0136782
TCCGATC47600.026.9380443
CCGATCT47750.026.6178674
CTTCCGA49250.026.1263351
CGGTCGA450.001227543324.99785420
CGATCTA8200.023.318645
TTAACGG2700.021.66903935
CGATCTT9150.021.6351415
GACGTGT10300.021.18566310
GAGACGT10900.021.0509578
CGATCTC16500.020.8595855
ACGTGTG11250.019.39665211
CGGTACC3250.019.3934745
GTATTAG9450.017.6152421
GGAGACG13550.017.5980577
AGACGTG12450.017.3460279
ACCGTCG1953.45608E-1117.304338
CGTGTGC13250.017.14798412
GGGAGAC14800.016.4157316
Produced by FastQC (version 0.11.2)