FastQCFastQC Report
Thu 20 Jul 2017
HT5LJBCXY_l02n02_inf_cell78.3520000009595c.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHT5LJBCXY_l02n02_inf_cell78.3520000009595c.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2151405
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCCTATACCTTCTGCATAATGAATTAACTAGAAATAACTTTGCAAGGAGA22320.10374615658139681No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCCGATC37350.028.8522153
TTCCGAT37550.028.5787162
CCGATCT38100.028.166164
CTTCCGA38700.027.9032291
CGATCTG17850.027.2238675
CGATCTA6750.026.9970045
GACGTGT7050.022.97670710
CGATCTT7650.021.4682035
ACGTGTG7850.020.9217311
CGATCTC12450.020.7808255
GAGACGT8450.020.5006838
TTAGCGA600.006512977618.74966227
GGAGACG9900.018.6342937
AGACGTG8950.017.847189
TTAACGG2053.6379788E-1217.56106635
GATCTAG4150.016.8053636
GTATTAG7850.016.04881
GGGAGAC11750.015.8918526
CGTGTGC10450.015.50103212
CCGCTAG4000.015.18757836
Produced by FastQC (version 0.11.2)