FastQCFastQC Report
Thu 20 Jul 2017
HT5LJBCXY_l02n02_inf_cell77.35200000095925.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHT5LJBCXY_l02n02_inf_cell77.35200000095925.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1750253
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGATCTA5000.028.7975
TCCGATC32950.028.1980783
CCGATCT33000.028.0871834
CGATCTG15950.028.0691765
TTCCGAT33800.027.5555152
CTTCCGA34050.027.485341
TAACGGG551.3693041E-424.543612
CGATCTT6250.023.3975625
CGATCTC9500.020.1294825
GACGTGT6050.019.70925310
GAGACGT7000.019.60518
GTCGACG706.9702265E-419.29197543
GATCTAG3350.018.8040126
ATCTGCG2650.018.67737
TACACCG1651.2332748E-917.7254285
AGACGTG7150.017.3058919
ACGTGTG6500.016.96025811
GTATTAG6100.016.5961591
TAGGGGT3650.016.025274
CGTCGAC1004.159219E-415.75466142
Produced by FastQC (version 0.11.2)