FastQCFastQC Report
Thu 20 Jul 2017
HT5LJBCXY_l02n02_inf_cell76.3520000009590b.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHT5LJBCXY_l02n02_inf_cell76.3520000009590b.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2213755
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCCTATACCTTCTGCATAATGAATTAACTAGAAATAACTTTGCAAGGAGA23490.10610930297164772No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTCCGA45900.028.9672431
TTCCGAT46400.028.6066112
TCCGATC46500.028.5934723
CCGATCT46500.028.3515644
CGATCTG22550.028.3337575
CGATCTA7250.027.6175025
CGATCTT7550.023.242355
GACGCGT702.7007336E-522.50401139
GACGTGT7000.021.21183810
CGATCTC14800.020.8252985
GAGACGT8250.019.9067948
GTCGACG807.543569E-519.69545743
ACGCGTG706.9757714E-419.29002240
ACGTGTG7950.018.11111611
AGACGTG8400.017.9443579
TAGCGTA2300.017.6066657
ATAGCGT2450.017.4469646
GATCTGG16000.017.2948786
CCGTCGA800.001708964716.8787741
ATCTGCG3350.016.7891047
Produced by FastQC (version 0.11.2)