FastQCFastQC Report
Thu 20 Jul 2017
HT5LJBCXY_l02n02_inf_cell75.352000000958fd.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHT5LJBCXY_l02n02_inf_cell75.352000000958fd.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3369050
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCCTATACCTTCTGCATAATGAATTAACTAGAAATAACTTTGCAAGGAGA62430.18530446268235853No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAAT47670.14149389293717812No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGATT39520.11730309731229872No Hit
TCTATAGGGTGATAGATTGGTCCAATTGGGTGTGAGGAGTTCAGTTATATG38780.11510663243347531No Hit
GAATAATGGAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGG37750.11204939077781571No Hit
ATATAGTACCGCAAGGGAAAGATGAAAAATTATAACCAAGCATAATATAGC35540.10548967809916741No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGATCTG40350.030.1618255
CTTCCGA79750.029.7312931
CCGATCT81200.028.7571074
TCCGATC82350.028.574063
CGATCTA13600.028.119915
TTCCGAT89200.026.531072
CGATCTT11350.022.1986225
GACGTGT8450.021.03200510
CGATCTC23350.020.9062775
GATCTGG27850.019.6284036
GATCTAG7600.019.5359366
GATCTGC17850.019.408256
GTCGACG750.001107756518.0074343
ATCTGGG17750.017.996747
GCTCTTC30050.017.8919353
TCTTCCG31100.017.721875
GATCTGT10300.017.6909716
GTATATA10700.017.6603531
TCTGCGC3850.017.5292938
GGTACTA7050.017.23859839
Produced by FastQC (version 0.11.2)