FastQCFastQC Report
Thu 20 Jul 2017
HT5LJBCXY_l02n02_inf_cell74.352000000958d3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHT5LJBCXY_l02n02_inf_cell74.352000000958d3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1491640
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCCTATACCTTCTGCATAATGAATTAACTAGAAATAACTTTGCAAGGAGA23870.16002520715454133No Hit
ATATAGTACCGCAAGGGAAAGATGAAAAATTATAACCAAGCATAATATAGC15450.10357727065511786No Hit
TCTATAGGGTGATAGATTGGTCCAATTGGGTGTGAGGAGTTCAGTTATATG14990.10049341664208522No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCCGATC30750.029.1170253
CGATCTA5200.028.9854835
CTTCCGA31450.028.5395281
CCGATCT31150.028.5264764
CGATCTG15300.028.3775545
TTCCGAT32150.027.9181372
CGGTACC2000.021.38500645
CGATCTT5450.021.0514875
GACGTGT4600.021.02975810
CGATCTC9100.021.012935
TTAACGG2250.018.99997135
GCTCTAT3150.018.5676651
GATCTAC3050.018.4395056
GTATTAG6850.018.0620331
GATCTGT5400.017.9136376
AGACGTG5550.017.8348229
GAGACGT6250.017.6370168
CGTGTGC6350.017.3598512
ACGTGTG5750.017.21505711
GATCTAG3850.016.9451876
Produced by FastQC (version 0.11.2)