FastQCFastQC Report
Thu 20 Jul 2017
HT5LJBCXY_l02n02_inf_cell73.352000000958b9.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHT5LJBCXY_l02n02_inf_cell73.352000000958b9.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1598670
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCCTATACCTTCTGCATAATGAATTAACTAGAAATAACTTTGCAAGGAGA23010.14393214359436282No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTCCGA31150.031.7049061
CGATCTA6150.031.4608575
TCCGATC32150.030.2998543
CCGATCT32100.030.1367934
CGATCTG15150.029.403555
TTCCGAT33750.028.9300772
CTATGCG450.001227346224.9979679
CGGGATA450.001227346224.99796715
CGATCTC9500.023.9191075
GTCGACG500.002260304822.50802643
TCTTCCG10550.020.4722675
CGTCGAC550.003931211320.45992342
CGATCTT5150.019.6585965
TCCCCCG6250.019.0784521
CCCGATG6450.018.83567824
CGTCCGA1454.771209E-918.62208737
GATCTAG4100.018.1082846
CCGTCGA750.001109456118.00247841
ATCTCGC2001.8189894E-1217.9985357
GCTCTAT3000.017.997411
Produced by FastQC (version 0.11.2)