FastQCFastQC Report
Thu 20 Jul 2017
HT5LJBCXY_l02n02_inf_cell72.35200000095883.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHT5LJBCXY_l02n02_inf_cell72.35200000095883.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3023334
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCCTATACCTTCTGCATAATGAATTAACTAGAAATAACTTTGCAAGGAGA53380.1765600492701104No Hit
TCTATAGGGTGATAGATTGGTCCAATTGGGTGTGAGGAGTTCAGTTATATG31280.10346193969968255No Hit
ATATAGTACCGCAAGGGAAAGATGAAAAATTATAACCAAGCATAATATAGC30730.10164275597734157No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCCGATC57200.031.2676833
CTTCCGA57950.031.2118851
TTCCGAT58150.030.7568592
CCGATCT58700.030.2387244
CGATCTG30850.030.1175375
CGATCTA9200.026.164995
CGATCTC16500.023.8604555
CGATCTT9350.023.3391325
ATCTGCG5450.021.0522377
CTACGCG706.993351E-419.283149
GATCTGC16650.018.7812796
TACGCGG600.00651791318.747510
GGTACTA4950.018.18480539
CGGTACT5200.017.743338
GATCTAG6600.017.7249096
CGTCGTA3600.017.49766510
ACCGTCG3950.017.0863298
CCGTCGT4300.016.7419539
TTGCGGT5650.016.32903535
TCTTCCG21800.016.3051645
Produced by FastQC (version 0.11.2)