FastQCFastQC Report
Thu 20 Jul 2017
HT5LJBCXY_l02n02_inf_cell71.35200000095869.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHT5LJBCXY_l02n02_inf_cell71.35200000095869.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1790351
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCCTATACCTTCTGCATAATGAATTAACTAGAAATAACTTTGCAAGGAGA21020.11740714530279257No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGATCTA5500.026.1778875
TCCGATC36000.025.4954573
CCGATCT36350.025.1261984
TTCCGAT36950.024.8399582
CGATCTG17350.024.6360685
GAGACGT10700.024.3888598
CTTCCGA38200.024.1449131
GACGTGT10700.022.28637110
ACGTGTG10750.021.97344211
CGATCTT6750.021.3301285
GGGAGAC13450.020.740386
AGACGTG11500.020.5403929
GGAGACG13900.019.9070827
TCGTCTA706.9821096E-419.28712732
CGTGTGC13600.018.36121212
CGATCTC11450.018.0758885
CGGTACC2150.017.79796845
GGCGTTA2350.017.23819442
GACGTTT1257.641824E-616.19756710
GATCTCG5800.015.9027836
Produced by FastQC (version 0.11.2)