Basic Statistics
Measure | Value |
---|---|
Filename | HT5LJBCXY_l02n02_inf_cell70.35200000095840.fastq.gz |
File type | Conventional base calls |
Encoding | Sanger / Illumina 1.9 |
Total Sequences | 115746 |
Sequences flagged as poor quality | 0 |
Sequence length | 51 |
%GC | 52 |
Per base sequence quality
Per tile sequence quality
Per sequence quality scores
Per base sequence content
Per sequence GC content
Per base N content
Sequence Length Distribution
Sequence Duplication Levels
Overrepresented sequences
Sequence | Count | Percentage | Possible Source |
---|---|---|---|
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA | 1678 | 1.4497261244449051 | No Hit |
CTTCCGATCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT | 231 | 0.19957493131512105 | No Hit |
GTGCTCTTCCGATCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT | 229 | 0.19784700983187326 | No Hit |
CGTCTAGATCGGAAGAGCACACGTCTAGATCGGAAGAGCACACGTCTAGAT | 150 | 0.12959411124358508 | Illumina Multiplexing PCR Primer 2.01 (95% over 21bp) |
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT | 146 | 0.1261382682770895 | No Hit |
Adapter Content
Kmer Content
Sequence | Count | PValue | Obs/Exp Max | Max Obs/Exp Position |
---|---|---|---|---|
AGGTATA | 20 | 7.001998E-4 | 45.00649 | 34 |
CAGGTAT | 20 | 7.001998E-4 | 45.00649 | 33 |
ACGCATG | 20 | 7.001998E-4 | 45.00649 | 40 |
ACACCCG | 35 | 1.19795E-7 | 45.00649 | 19 |
AGGCCGA | 20 | 7.001998E-4 | 45.00649 | 34 |
CCGAGTG | 20 | 7.001998E-4 | 45.00649 | 40 |
GGTATAC | 35 | 1.19795E-7 | 45.00649 | 35 |
TCGCGTG | 20 | 7.001998E-4 | 45.00649 | 29 |
TCCTGTT | 25 | 3.8657185E-5 | 45.006485 | 21 |
TGTGGAC | 25 | 3.8657185E-5 | 45.006485 | 43 |
GTATACG | 30 | 2.1464184E-6 | 45.006485 | 36 |
GGCTGTT | 45 | 3.783498E-10 | 45.006485 | 45 |
ACGCTTG | 25 | 3.8756087E-5 | 44.987038 | 11 |
TCCGTTC | 130 | 0.0 | 41.54445 | 22 |
CGTTCTG | 50 | 1.0622898E-9 | 40.505836 | 24 |
TCTCGTC | 85 | 0.0 | 39.711605 | 27 |
ATCTCGG | 40 | 3.4185177E-7 | 39.380676 | 26 |
TCTGGCC | 75 | 0.0 | 39.00562 | 27 |
GGGAGCG | 35 | 6.1935607E-6 | 38.57699 | 35 |
ATACGCG | 35 | 6.1935607E-6 | 38.57699 | 38 |