FastQCFastQC Report
Thu 20 Jul 2017
HT5LJBCXY_l02n02_inf_cell70.35200000095840.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHT5LJBCXY_l02n02_inf_cell70.35200000095840.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences115746
Sequences flagged as poor quality0
Sequence length51
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA16781.4497261244449051No Hit
CTTCCGATCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2310.19957493131512105No Hit
GTGCTCTTCCGATCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2290.19784700983187326No Hit
CGTCTAGATCGGAAGAGCACACGTCTAGATCGGAAGAGCACACGTCTAGAT1500.12959411124358508Illumina Multiplexing PCR Primer 2.01 (95% over 21bp)
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1460.1261382682770895No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGGTATA207.001998E-445.0064934
CAGGTAT207.001998E-445.0064933
ACGCATG207.001998E-445.0064940
ACACCCG351.19795E-745.0064919
AGGCCGA207.001998E-445.0064934
CCGAGTG207.001998E-445.0064940
GGTATAC351.19795E-745.0064935
TCGCGTG207.001998E-445.0064929
TCCTGTT253.8657185E-545.00648521
TGTGGAC253.8657185E-545.00648543
GTATACG302.1464184E-645.00648536
GGCTGTT453.783498E-1045.00648545
ACGCTTG253.8756087E-544.98703811
TCCGTTC1300.041.5444522
CGTTCTG501.0622898E-940.50583624
TCTCGTC850.039.71160527
ATCTCGG403.4185177E-739.38067626
TCTGGCC750.039.0056227
GGGAGCG356.1935607E-638.5769935
ATACGCG356.1935607E-638.5769938