FastQCFastQC Report
Thu 20 Jul 2017
HT5LJBCXY_l02n02_inf_cell7.35200000095061.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHT5LJBCXY_l02n02_inf_cell7.35200000095061.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1662564
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCCTATACCTTCTGCATAATGAATTAACTAGAAATAACTTTGCAAGGAGA35690.21466842780187712No Hit
ATATAGTACCGCAAGGGAAAGATGAAAAATTATAACCAAGCATAATATAGC23240.13978409252215251No Hit
TCTATAGGGTGATAGATTGGTCCAATTGGGTGTGAGGAGTTCAGTTATATG23100.13894201967563352No Hit
GCATAATATAGCAAGGACTAACCCCTATACCTTCTGCATAATGAATTAACT18360.11043183901491914No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTCCGA37400.030.6166041
CGATCTG18500.030.4003875
TCCGATC37600.030.2742563
TTCCGAT37700.030.1342832
CCGATCT38500.029.5081164
CGATCTA6250.027.3554845
CGATCTC11800.023.4495185
GATCTGG11400.022.298956
GATCTCG5050.022.2735526
CGATCTT6250.020.8765535
ATCTGCG2950.019.8272347
CGCCTAT1454.774847E-918.62153636
TATCGCC1454.776666E-918.62097733
TTGCGGT3700.018.24407235
GATCTGC10150.018.174346
CGGTACC3100.018.1525445
TACGTGA1507.2668627E-918.00081836
CGATCCT1002.0472578E-518.00027728
TACCGAA1002.0504387E-517.99702811
TCTTCCG13050.017.755695
Produced by FastQC (version 0.11.2)