FastQCFastQC Report
Thu 20 Jul 2017
HT5LJBCXY_l02n02_inf_cell69.35200000095833.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHT5LJBCXY_l02n02_inf_cell69.35200000095833.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1899109
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCCGATC43350.028.335023
CCGATCT43800.027.889824
TTCCGAT44350.027.64542
CTTCCGA45550.027.3122041
CGATCTG19100.027.0903435
CGATCTA7450.025.9694165
CGATCTT8650.022.8868795
GACGTGT9950.022.60981210
GAGACGT10700.022.4967638
CGATCTC14100.022.4967615
ACGTGTG10050.022.16098811
GGAGACG12300.020.1190537
CGTGTGC11500.019.17165812
AGACGTG11800.019.0650529
TAACCGC600.00651743818.7473016
GGGAGAC14400.017.8099376
TATTACG1153.4558052E-617.6061612
CCGTCGG2350.017.23791540
GATCTGC10800.016.247666
AACGGCA850.002564849815.88341232
Produced by FastQC (version 0.11.2)