FastQCFastQC Report
Thu 20 Jul 2017
HT5LJBCXY_l02n02_inf_cell68.35200000095819.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHT5LJBCXY_l02n02_inf_cell68.35200000095819.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1675033
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTCCGAT34550.027.0852972
CGATCTA5250.026.9941445
TCCGATC34700.026.9033853
CCGATCT35400.026.4984874
CTTCCGA36150.026.2598631
CGATCTT7500.026.0943375
CGATCTG15350.025.4993535
CGCCTAT551.3680867E-424.54672434
GAGACGT11100.021.8877968
GACGTGT10050.021.48915910
ACGTGTG10500.020.78244811
AGACGTG11450.019.450519
CGCATCG2350.019.14592713
GGCGTTA2000.019.13627442
GGAGACG13450.018.8992467
GGGAGAC13850.018.840686
CGATCTC11500.018.7785365
TCCCGTA600.006517622618.74705317
CGTGTGC12450.018.43083812
TAACGCC2550.017.643234
Produced by FastQC (version 0.11.2)