FastQCFastQC Report
Thu 20 Jul 2017
HT5LJBCXY_l02n02_inf_cell67.352000000957fe.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHT5LJBCXY_l02n02_inf_cell67.352000000957fe.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1803561
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTCCGAT30800.028.2681962
CTTCCGA31700.027.749511
TCCGATC31300.027.744753
CCGATCT31900.027.2229024
CGATCTG15050.026.6085595
CGATCTA6150.025.2413065
GAGACGT5500.022.4976848
GACGTGT4950.021.36143910
CGATCTT5400.020.8311885
CGATCTC9850.020.5562615
ACGTGTG5500.018.81624611
TTAACGG1651.2314558E-917.72790735
GATCTAG3850.017.5306646
GTATAGA2850.017.3666341
GTACGAT800.001712215516.87373220
GGAGACG8150.016.5627127
AGACGTG6450.016.3936619
GGGAGAC8900.015.4197636
GTATATA7500.015.2984261
CCCCTAT8000.015.1859381
Produced by FastQC (version 0.11.2)