FastQCFastQC Report
Thu 20 Jul 2017
HT5LJBCXY_l02n02_inf_cell66.352000000957d4.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHT5LJBCXY_l02n02_inf_cell66.352000000957d4.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1639165
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTCCGAT30900.028.248332
TCCGATC30800.028.1939623
CGATCTG14450.028.0236175
CGATCTA5250.027.85315
CTTCCGA31850.027.6176571
CCGATCT31750.027.4212194
CGAGCGT754.609319E-520.9969546
CGATCTT5650.020.704965
CGATCTC10400.020.1172735
CGGGTTA1703.6379788E-1219.86399843
TAAGCGT706.99203E-419.2829174
GACGTGT6150.019.02163110
GAGACGT6750.018.3306758
GATCTGG9700.018.3220846
ATCTGCG1851.6370905E-1118.2405977
GTATATA5600.018.0777341
ACGTGTG6250.017.99738911
AGACGTG6550.017.5165429
CGTAGAC901.8670535E-417.49906227
GTATTAA5800.017.066491
Produced by FastQC (version 0.11.2)