FastQCFastQC Report
Thu 20 Jul 2017
HT5LJBCXY_l02n02_inf_cell65.352000000957ae.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHT5LJBCXY_l02n02_inf_cell65.352000000957ae.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1592387
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGATCTG13200.029.8242175
TCCGATC29700.027.949553
TTCCGAT29800.027.855762
CCGATCT30050.027.5491524
CGATCTA4450.027.2984945
CTTCCGA31600.026.9097481
CGATCTT5750.021.5178955
CGATCTC10050.018.8026125
ACGTGTG4700.018.66687811
GCGATTC750.001109002518.00356938
TCTTCCG13100.017.8594065
GACGTGT4750.017.52318410
TACCGTC2350.017.2309657
GAGACGT5050.016.927678
GTATTAA4800.016.403321
ATCTGCG2500.016.1971077
CGTCGTA1956.511982E-1016.1509610
GATCTGC7900.015.9465186
AGACGTG5350.015.5579679
GGCGTTA1907.952622E-915.402143542
Produced by FastQC (version 0.11.2)