FastQCFastQC Report
Thu 20 Jul 2017
HT5LJBCXY_l02n02_inf_cell64.35200000095784.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHT5LJBCXY_l02n02_inf_cell64.35200000095784.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2000245
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCCTATACCTTCTGCATAATGAATTAACTAGAAATAACTTTGCAAGGAGA27290.13643328692235202No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTCCGAT42350.029.8005432
TCCGATC42250.029.711343
CCGATCT42700.029.1874794
CGATCTG22700.028.9382115
CTTCCGA43850.028.729841
CGATCTA5950.028.3569165
CGATCTC13200.023.17825
CGATCTT6000.020.9967215
ACGATCG654.2413487E-420.76806425
ATCGTAC550.003938821620.45339825
TATAGTG2950.019.064825
GATCTCG6300.017.8543556
GATCTGT7850.017.4813466
GATCTGG15550.017.3606326
GATCTGC11700.016.535886
ATCTACG2057.4578566E-1116.4608467
GACGTGT5650.016.32488610
GGCGTTA2550.015.88781642
GTTAGAC1004.1739162E-415.7475413
GCCGTAT1156.7694964E-515.656774539
Produced by FastQC (version 0.11.2)