FastQCFastQC Report
Thu 20 Jul 2017
HT5LJBCXY_l02n02_inf_cell63.3520000009576a.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHT5LJBCXY_l02n02_inf_cell63.3520000009576a.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1770314
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCCTATACCTTCTGCATAATGAATTAACTAGAAATAACTTTGCAAGGAGA19690.1112232067305574No Hit
CCCTTATACTGGAGACCCTCCTTACAGCCATGGGACAGGAACAGGATACAC19590.1106583351879949No Hit
ATACTGGATATCCCGACTGGTCTTCTGTATGAACTGCTGGGGAAGAATTTT18240.10303256936340106No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTCCGA37400.029.4158251
CGATCTG19050.029.1706285
CCGATCT37200.029.0901494
TCCGATC37800.028.7474383
TTCCGAT39850.027.3250452
CGTCTAA507.152505E-527.00445740
CGATCTA4400.026.077225
CCGAATA450.001227454324.99777212
CGATCTT7150.022.969985
CGATCTC11150.022.800665
AATTGCG602.4635982E-422.5062643
ATTCGAC2650.021.2245241
TCGACAC2700.020.8314763
TAGCCGA550.00393939820.4527239
AAACGCA2250.020.00047734
CGCATCA2350.019.14993337
CGGACGT600.006509895418.75097836
CGGATTG1507.2577677E-918.00297242
GATCTGC8250.017.7256936
AACGCAT2550.017.6474835
Produced by FastQC (version 0.11.2)